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. 1980 Dec;32(3):1043–1049. doi: 10.1016/S0006-3495(80)85035-1

[1H]Spin-echo nuclear magnetic resonance in plant tissue. I. The effect of Mn(II) and water content in wheat leaves.

H van As, W P van Vliet, T J Schaafsma
PMCID: PMC1327390  PMID: 6266537

Abstract

The effect of age-dependent Mn(II)-gradients, as observed by electron paramagnetic resonance (EPR), on the [1H]NMR spin-spin relaxation time (T2) was studied in wheat leaves. A non-exponential T2 spin-echo decay was always observed, revealing the presence of at least two different fractions of non- (or slowly) exchanging water in the leaves. No effect of the Mn(II)-concentration on T2 of the separate water fractions (covering approximately 90% of the total water content) has been found. From these observations we conclude that Mn(II) is present in bound form. The dependence of T2 on water content can be explained with a two-state model, demonstrating the occurrence of fast exchange within each of the two slowly exchanging water fractions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cooke R., Wien R. The state of water in muscle tissue as determined by proton nuclear magnetic resonance. Biophys J. 1971 Dec;11(12):1002–1017. doi: 10.1016/S0006-3495(71)86274-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fedotov V. D., Miftakhutdinova F. G., Murtazin Sh F. Issledovanie protonnoi relaksatsii v zhivykh rastitel'nykh tkaniakh metodom spinovogo ékha. Biofizika. 1969 Sep-Oct;14(5):873–882. [PubMed] [Google Scholar]
  3. Fung B. M. Correlation of relaxation time with water content in muscle and brain tissues. Biochim Biophys Acta. 1977 Mar 29;497(1):317–322. doi: 10.1016/0304-4165(77)90165-9. [DOI] [PubMed] [Google Scholar]
  4. Hazlewood C. F., Chang D. C., Nichols B. L., Woessner D. E. Nuclear magnetic resonance transverse relaxation times of water protons in skeletal muscle. Biophys J. 1974 Aug;14(8):583–606. doi: 10.1016/S0006-3495(74)85937-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hughes C. B. An eclectic approach to parent group education. Nurs Clin North Am. 1977 Sep;12(3):469–479. [PubMed] [Google Scholar]
  6. Raaphorst G. P., Law P., Kruuv J. Water content and spin lattice relaxation times of cultured mammalian cells subjected to various salt, sucrose, or DMSO solutions. Physiol Chem Phys. 1978;10(2):177–191. [PubMed] [Google Scholar]
  7. Siderer Y., Malkin S., Poupko R., Luz Z. Electron spin resonance and photoreaction of Mn(II) in lettuce chloroplasts. Arch Biochem Biophys. 1977 Feb;179(1):174–182. doi: 10.1016/0003-9861(77)90101-1. [DOI] [PubMed] [Google Scholar]
  8. Stout D. G., Steponkus P. L. Nuclear magnetic resonance relaxation times and plasmalemma water exchange in ivy bark. Plant Physiol. 1978 Oct;62(4):636–641. doi: 10.1104/pp.62.4.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wydrzynski T. J., Marks S. B., Schmidt P. G., Govindjee, Gutowsky H. S. Nuclear magnetic relaxation by the manganese in aqueous suspensions of chloroplasts. Biochemistry. 1978 May 30;17(11):2155–2162. doi: 10.1021/bi00604a020. [DOI] [PubMed] [Google Scholar]

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