Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1979 Apr;26(1):1–21. doi: 10.1016/S0006-3495(79)85231-5

Electrophoresis and diffusion in the plane of the cell membrane.

M Poo, J W Lam, N Orida, A W Chao
PMCID: PMC1328499  PMID: 262406

Abstract

Electrophoretic and diffusional movements of concanavalin A (Con A) receptors and acetylcholine (ACh) receptors in the plane of the plasma membrane of mononucleate, spherical Xenopus myoblasts were studied by microfluorimetry and iontophoresis. We found that (a) a uniform electric field of 10 V/cm applied along the cell surface produces a partial accumulation of both types of receptors toward the cathodal pole of the cell within 30 min: (b) post-field relaxation of the culture results in the complete recovery of the uniform distribution of the Con A receptors within 10 min; and (c) in contrast to the Con A receptor in general, accumulation of ACh receptors by the electric field results in the formation of stable, localized receptor aggregates. Theoretical analyses were carried out for the distribution of charged membrane receptors at equilibrium between electrophoresis and diffusion, and for the rate of back diffusion after the removal of the field. These analyses indicated that, at 22 degrees C, the average electrophoretic mobility of the electrophoretically mobile population of the Con A receptors is about 1.9 X 10(-3) micron/s per V/cm, while their average diffusion coefficient is 5.1 X 10(-9) cm2/s.

Full text

PDF
1

Images in this article

17FIGURE 11
on p.17

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelrod D., Ravdin P., Koppel D. E., Schlessinger J., Webb W. W., Elson E. L., Podleski T. R. Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4594–4598. doi: 10.1073/pnas.73.12.4594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brockes J. P., Hall Z. W. Acetylcholine receptors in normal and denervated rat diaphragm muscle. II. Comparison of junctional and extrajunctional receptors. Biochemistry. 1975 May 20;14(10):2100–2106. doi: 10.1021/bi00681a009. [DOI] [PubMed] [Google Scholar]
  3. EYLAR E. H., MADOFF M. A., BRODY O. V., ONCLEY J. L. The contribution of sialic acid to the surface charge of the erythrocyte. J Biol Chem. 1962 Jun;237:1992–2000. [PubMed] [Google Scholar]
  4. Edidin M., Fambrough D. Fluidity of the surface of cultured muscle fibers. Rapid lateral diffusion of marked surface antigens. J Cell Biol. 1973 Apr;57(1):27–37. doi: 10.1083/jcb.57.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fowler V., Branton D. Lateral mobility of human erythrocyte integral membrane proteins. Nature. 1977 Jul 7;268(5615):23–26. doi: 10.1038/268023a0. [DOI] [PubMed] [Google Scholar]
  6. Frye L. D., Edidin M. The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J Cell Sci. 1970 Sep;7(2):319–335. doi: 10.1242/jcs.7.2.319. [DOI] [PubMed] [Google Scholar]
  7. Jacobson K., Wu E., Poste G. Measurement of the translational mobility of concanavalin A in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleaching. Biochim Biophys Acta. 1976 Apr 16;433(1):215–222. doi: 10.1016/0005-2736(76)90189-9. [DOI] [PubMed] [Google Scholar]
  8. Jaffe L. F. Electrophoresis along cell membranes. Nature. 1977 Feb 17;265(5595):600–602. doi: 10.1038/265600a0. [DOI] [PubMed] [Google Scholar]
  9. Poo M. M., Poo W. J., Lam J. W. Lateral electrophoresis and diffusion of Concanavalin A receptors in the membrane of embryonic muscle cell. J Cell Biol. 1978 Feb;76(2):483–501. doi: 10.1083/jcb.76.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Poo M., Robinson K. R. Electrophoresis of concanavalin A receptors along embryonic muscle cell membrane. Nature. 1977 Feb 17;265(5595):602–605. doi: 10.1038/265602a0. [DOI] [PubMed] [Google Scholar]
  11. Schlessinger J., Barak L. S., Hammes G. G., Yamada K. M., Pastan I., Webb W. W., Elson E. L. Mobility and distribution of a cell surface glycoprotein and its interaction with other membrane components. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2909–2913. doi: 10.1073/pnas.74.7.2909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schlessinger J., Koppel D. E., Axelrod D., Jacobson K., Webb W. W., Elson E. L. Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2409–2413. doi: 10.1073/pnas.73.7.2409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zagyansky Y., Edidin M. Lateral diffusion of concanavalin A receptors in the plasma membrane of mouse fibroblasts. Biochim Biophys Acta. 1976 Apr 16;433(1):209–214. doi: 10.1016/0005-2736(76)90188-7. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES