Abstract
A mathematical model of the fluorescence decay experiment based on linear systems theory is presented. The model suggests an experimental technique that increases the probability of correctly determining the decay constants of a multicomponent system. The use of moment methods for data analysis improves accuracy by combining information obtained from several discrete experiments. Examples are presented to show that the analysis of a three component system composed of known standards is improved as the number of experimental determinations is increased from one to four. The discrete measurements are made by changing the excitation and emission wavelengths.
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Selected References
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- Gafni A., Modlin R. L., Brand L. Analysis of fluorescence decay curves by means of the Laplace transformation. Biophys J. 1975 Mar;15(3):263–280. doi: 10.1016/S0006-3495(75)85817-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grinvald A. The use of standards in the analysis of fluorescence decay data. Anal Biochem. 1976 Sep;75(1):260–280. doi: 10.1016/0003-2697(76)90077-4. [DOI] [PubMed] [Google Scholar]
- Isenberg I., Dyson R. D., Hanson R. Studies on the analysis of fluorescence decay data by the method of moments. Biophys J. 1973 Oct;13(10):1090–1115. doi: 10.1016/S0006-3495(73)86047-3. [DOI] [PMC free article] [PubMed] [Google Scholar]