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. 1979 Aug;27(2):187–191. doi: 10.1016/S0006-3495(79)85210-8

Motility evaluation of human spermatozoa by photon correlation spectroscopy.

R Finsy, J Peetermans, H Lekkerkerker
PMCID: PMC1328577  PMID: 262432

Abstract

The application of photon correlation spectroscopy for the evaluation of motility parameters of undiluted human sperm is investigated. Measurements on semen samples, selected visually as good (i.e., fraction motile spermatozoa larger than 0.6 and a positive appreciation of the motion), gave estimates of the fraction motile spermatozoa, reproducible within 10%, and of the mean velocity of the motile cells, reproducible within 5%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berne B. J., Nossal R. Inelastic light scattering by large structured particles. Biophys J. 1974 Nov;14(11):865–880. doi: 10.1016/S0006-3495(74)85955-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cooke D. F., Hallett F. R., Barker C. A. Motility evaluation of bull spermatozoa by photon correlation spectroscopy. J Mechanochem Cell Motil. 1976;3(4):219–223. [PubMed] [Google Scholar]
  3. Hallett F. R., Craig T., Marsh J. Swimming speed distributions of bull spermatozoa as determined by quasi-elastic light scattering. Biophys J. 1978 Mar;21(3):203–216. doi: 10.1016/S0006-3495(78)85520-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jouannet P., Volochine B., Deguent P., Serres C., David G. Light scattering determination of various characteristic paramenters of spermatozoa motility in a serie of human sperm. Andrologia. 1977 Jan-Mar;9(1):36–49. doi: 10.1111/j.1439-0272.1977.tb01257.x. [DOI] [PubMed] [Google Scholar]
  5. Nossal R. Spectral analysis of laser light scattered from motile microorganisms. Biophys J. 1971 Apr;11(4):341–354. doi: 10.1016/S0006-3495(71)86219-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stock G. B. Application of splines to the calculation of bacterial swimming speed distributions. Biophys J. 1976 May;16(5):535–540. doi: 10.1016/S0006-3495(76)85708-6. [DOI] [PMC free article] [PubMed] [Google Scholar]

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