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. 1979 Nov;28(2):185–196. doi: 10.1016/S0006-3495(79)85170-X

Effect of benzyl alcohol on lipid bilayers. A comparisons of bilayer systems.

L Ebihara, J E Hall, R C MacDonald, T J McIntosh, S A Simon
PMCID: PMC1328624  PMID: 263698

Abstract

The effect of the small anesthetic molecule, benzyl alcohol, on the structure of various bilayer system has been studied by optical, electrical, and x-ray diffraction techniques. We find that the modifications in bilayer thickness caused by benzyl alcohol differ dramatically for planar (or black lipid) bilayers containing solvent, planar bilayers containing little or no solvent, and vesicular bilayers. Benzyl alcohol increases the thickness of planar bilayers containing n-alkane solvents, yet decreases the thickness of "solvent-free" planar bilayers. The effect of benzyl alcohol on vesicular bilayers below the phase transition temperature also depends on whether solvent is present in the bilayers. Without solvent, gel-state bilayers are reduced in thickness by benzyl alcohol, whereas in the presence of solvent, the thickness is unchanged. Above the phase transition temperature, benzyl alcohol has no measurable effect on vesicular bilayer thickness, whether solvent is present or not. These results indicate that different model membrane systems respond quite differently to a particular anesthetic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashcroft R. G., Coster H. G., Smith J. R. Local anaesthetic benzyl alcohol increases membrane thickness. Nature. 1977 Oct 27;269(5631):819–820. doi: 10.1038/269819a0. [DOI] [PubMed] [Google Scholar]
  2. BANGHAM A. D., HEARD D. H., FLEMANS R., SEAMAN G. V. An apparatus for microelectrophoresis of small particles. Nature. 1958 Sep 6;182(4636):642–644. doi: 10.1038/182642a0. [DOI] [PubMed] [Google Scholar]
  3. Bangham A. D., Standish M. M., Watkins J. C. Diffusion of univalent ions across the lamellae of swollen phospholipids. J Mol Biol. 1965 Aug;13(1):238–252. doi: 10.1016/s0022-2836(65)80093-6. [DOI] [PubMed] [Google Scholar]
  4. Colley C. M., Metcalfe J. C. The localisation of small molecules in lipid bilayers. FEBS Lett. 1972 Aug 15;24(3):241–246. doi: 10.1016/0014-5793(72)80364-8. [DOI] [PubMed] [Google Scholar]
  5. Hill M. W. The effect of anaesthetic-like molecules on the phase transition in smectic mesophases of dipalmitoyllecithin. I. The normal alcohol up to C equals 9 and three inhalation anaesthetics. Biochim Biophys Acta. 1974 Jul 12;356(1):117–124. doi: 10.1016/0005-2736(74)90299-5. [DOI] [PubMed] [Google Scholar]
  6. Hui F. K., Barton P. G. Mesomorphic behaviour of some phospholipids with aliphatic alcohols and other non-ionic substances. Biochim Biophys Acta. 1973 Mar 8;296(3):510–517. doi: 10.1016/0005-2760(73)90111-2. [DOI] [PubMed] [Google Scholar]
  7. Katz Y., Diamond J. M. Thermodynamic constants for nonelectrolyte partition between dimyristoyl lecithin and water. J Membr Biol. 1974;17(2):101–120. doi: 10.1007/BF01870175. [DOI] [PubMed] [Google Scholar]
  8. Legaly G., Weiss A., Stuke E. Effect of double-bonds on bimolecular films in membrane models. Biochim Biophys Acta. 1977 Nov 1;470(3):331–341. doi: 10.1016/0005-2736(77)90125-0. [DOI] [PubMed] [Google Scholar]
  9. Lesslauer W., Cain J. E., Blasie J. K. X-ray diffraction studies of lecithin bimolecular leaflets with incorporated fluorescent probes. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1499–1503. doi: 10.1073/pnas.69.6.1499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mabrey S., Sturtevant J. M. Incorporation of saturated fatty acids into phosphatidylcholine bilayers. Biochim Biophys Acta. 1977 Mar 25;486(3):444–450. doi: 10.1016/0005-2760(77)90094-7. [DOI] [PubMed] [Google Scholar]
  11. Metcalfe J. C., Seeman P., Burgen A. S. The proton relaxation of benzyl alcohol in erythrocyte membranes. Mol Pharmacol. 1968 Jan;4(1):87–95. [PubMed] [Google Scholar]
  12. Montal M., Mueller P. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3561–3566. doi: 10.1073/pnas.69.12.3561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reyes J., Latorre R. Effect of the anesthetics benzyl alcohol and chloroform on bilayers made from monolayers. Biophys J. 1979 Nov;28(2):259–279. doi: 10.1016/S0006-3495(79)85175-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Simon S. A., Lis L. J., MacDonald R. C., Kauffman J. W. The noneffect of a large linear hydrocarbon, squalene, on the phosphatidylcholine packing structure. Biophys J. 1977 Jul;19(1):83–90. doi: 10.1016/S0006-3495(77)85570-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Simon S. A., Stone W. L., Busto-Latorre P. A thermodynamic study of the partition of n-hexane into phosphatidylcholine and phosphatidylcholine-cholesterol bilayers. Biochim Biophys Acta. 1977 Aug 1;468(3):378–388. doi: 10.1016/0005-2736(77)90289-9. [DOI] [PubMed] [Google Scholar]
  16. Tardieu A., Luzzati V., Reman F. C. Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases. J Mol Biol. 1973 Apr 25;75(4):711–733. doi: 10.1016/0022-2836(73)90303-3. [DOI] [PubMed] [Google Scholar]
  17. Turner G. L., Oldfield E. Effect of a local anaesthetic on hydrocarbon chain order in membranes. Nature. 1979 Feb 22;277(5698):669–670. doi: 10.1038/277669a0. [DOI] [PubMed] [Google Scholar]
  18. White S. H. Formation of "solvent-free" black lipid bilayer membranes from glyceryl monooleate dispersed in squalene. Biophys J. 1978 Sep;23(3):337–347. doi: 10.1016/S0006-3495(78)85453-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. White S. H., Petersen D. C., Simon S., Yafuso M. Formation of planar bilayer membranes from lipid monolayers. A critique. Biophys J. 1976 May;16(5):481–489. doi: 10.1016/S0006-3495(76)85703-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. White S. H. Studies of the physical chemistry of planar bilayer membranes using high-precision measurements of specific capacitance. Ann N Y Acad Sci. 1977 Dec 30;303:243–265. [PubMed] [Google Scholar]

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