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. 1979 Nov;28(2):281–291. doi: 10.1016/S0006-3495(79)85176-0

Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics.

D E Koppel
PMCID: PMC1328630  PMID: 262551

Abstract

A theoretical formulation and experimental methodology are presented for a new multipoint analysis of membrane translational dynamics. The redistribution of fluorescent probe after a localized photobleaching pulse is monitored at several locations by a focused laser beam sequentially scanned through the bleached area. The spatial information so obtained provides a unique sensitivity to possible systematic flow and a direct internal calibration of the characteristic transport distance. These capabilities are demonstrated with experimental data on a reconstituted multibilayer system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelrod D., Koppel D. E., Schlessinger J., Elson E., Webb W. W. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys J. 1976 Sep;16(9):1055–1069. doi: 10.1016/S0006-3495(76)85755-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Axelrod D., Ravdin P. M., Podleski T. R. Control of acetylcholine receptor mobility and distribution in cultured muscle membranes. A fluorescence study. Biochim Biophys Acta. 1978 Jul 20;511(1):23–38. doi: 10.1016/0005-2736(78)90062-7. [DOI] [PubMed] [Google Scholar]
  3. Axelrod D., Ravdin P., Koppel D. E., Schlessinger J., Webb W. W., Elson E. L., Podleski T. R. Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4594–4598. doi: 10.1073/pnas.73.12.4594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bretscher M. S. Directed lipid flow in cell membranes. Nature. 1976 Mar 4;260(5546):21–23. doi: 10.1038/260021a0. [DOI] [PubMed] [Google Scholar]
  5. Edelman G. M. Surface modulation in cell recognition and cell growth. Science. 1976 Apr 16;192(4236):218–226. doi: 10.1126/science.769162. [DOI] [PubMed] [Google Scholar]
  6. Edidin M. Rotational and translational diffusion in membranes. Annu Rev Biophys Bioeng. 1974;3(0):179–201. doi: 10.1146/annurev.bb.03.060174.001143. [DOI] [PubMed] [Google Scholar]
  7. Edidin M., Zagyansky Y., Lardner T. J. Measurement of membrane protein lateral diffusion in single cells. Science. 1976 Feb 6;191(4226):466–468. doi: 10.1126/science.1246629. [DOI] [PubMed] [Google Scholar]
  8. Fahey P. F., Webb W. W. Lateral diffusion in phospholipid bilayer membranes and multilamellar liquid crystals. Biochemistry. 1978 Jul 25;17(15):3046–3053. doi: 10.1021/bi00608a016. [DOI] [PubMed] [Google Scholar]
  9. Jacobson K., Derzko Z., Wu E. S., Hou Y., Poste G. Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleaching. J Supramol Struct. 1976;5(4):565(417)–576(428). doi: 10.1002/jss.400050411. [DOI] [PubMed] [Google Scholar]
  10. Jacobson K., Wu E., Poste G. Measurement of the translational mobility of concanavalin A in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleaching. Biochim Biophys Acta. 1976 Apr 16;433(1):215–222. doi: 10.1016/0005-2736(76)90189-9. [DOI] [PubMed] [Google Scholar]
  11. Johnson M., Edidin M. Lateral diffusion in plasma membrane of mouse egg is restricted after fertilisation. Nature. 1978 Mar 30;272(5652):448–450. doi: 10.1038/272448a0. [DOI] [PubMed] [Google Scholar]
  12. Koppel D. E., Axelrod D., Schlessinger J., Elson E. L., Webb W. W. Dynamics of fluorescence marker concentration as a probe of mobility. Biophys J. 1976 Nov;16(11):1315–1329. doi: 10.1016/S0006-3495(76)85776-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nicolson G. L. Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components. Biochim Biophys Acta. 1976 Apr 13;457(1):57–108. doi: 10.1016/0304-4157(76)90014-9. [DOI] [PubMed] [Google Scholar]
  14. Peters R., Peters J., Tews K. H., Bähr W. A microfluorimetric study of translational diffusion in erythrocyte membranes. Biochim Biophys Acta. 1974 Nov 15;367(3):282–294. doi: 10.1016/0005-2736(74)90085-6. [DOI] [PubMed] [Google Scholar]
  15. Schlessinger J., Axelrod D., Koppel D. E., Webb W. W., Elson E. L. Lateral transport of a lipid probe and labeled proteins on a cell membrane. Science. 1977 Jan 21;195(4275):307–309. doi: 10.1126/science.556653. [DOI] [PubMed] [Google Scholar]
  16. Schlessinger J., Elson E. L., Webb W. W., Yahara I., Rutishauser U., Edelman G. M. Receptor diffusion on cell surfaces modulated by locally bound concanavalin A. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1110–1114. doi: 10.1073/pnas.74.3.1110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schlessinger J., Koppel D. E., Axelrod D., Jacobson K., Webb W. W., Elson E. L. Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2409–2413. doi: 10.1073/pnas.73.7.2409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shinitzky M., Inbar M. Microviscosity parameters and protein mobility in biological membranes. Biochim Biophys Acta. 1976 Apr 16;433(1):133–149. doi: 10.1016/0005-2736(76)90183-8. [DOI] [PubMed] [Google Scholar]
  19. Smith B. A., McConnell H. M. Determination of molecular motion in membranes using periodic pattern photobleaching. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759–2763. doi: 10.1073/pnas.75.6.2759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wolf D. E., Schlessinger J., Elson E. L., Webb W. W., Blumenthal R., Henkart P. Diffusion and patching of macromolecules on planar lipid bilayer membranes. Biochemistry. 1977 Jul 26;16(15):3476–3483. doi: 10.1021/bi00634a029. [DOI] [PubMed] [Google Scholar]
  21. Wu E. S., Jacobson K., Szoka F., Portis A., Jr Lateral diffusion of a hydrophobic peptide, N-4-nitrobenz-2-oxa-1,3-diazole gramicidin S, in phospholipid multibilayers. Biochemistry. 1978 Dec 12;17(25):5543–5550. doi: 10.1021/bi00618a033. [DOI] [PubMed] [Google Scholar]

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