Abstract
The time-dependent recovery of an elongated red cell is studied as a function of temperature. Before release, the elongated cell is in static equilibrium where external forces are balanced by surface elastic force resultants. Upon release, the cell recovers its initial shape with a time-dependent exponential behavior characteristic of a viscoelastic solid material undergoing large ("finite") deformation. The recovery process is characterized by a time constant, tc, that decreases from approximately 0.27 s at 6 degrees C to 0.06 s at 37 degrees C. From this measurement of the time constant and an independent measurement of the shear modulus of surface elasticity for red cell membrane, the value for the membrane surface viscosity as a function of temperature can be calculated.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cokelet G. R., Meiselman H. J. Rheological comparison of hemoglobin solutions and erythrocyte suspensions. Science. 1968 Oct 11;162(3850):275–277. doi: 10.1126/science.162.3850.275. [DOI] [PubMed] [Google Scholar]
- Evans E. A., Hochmuth R. M. Membrane viscoelasticity. Biophys J. 1976 Jan;16(1):1–11. doi: 10.1016/S0006-3495(76)85658-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans E. A. New membrane concept applied to the analysis of fluid shear- and micropipette-deformed red blood cells. Biophys J. 1973 Sep;13(9):941–954. doi: 10.1016/S0006-3495(73)86036-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hochmuth R. M., Worthy P. R., Evans E. A. Red cell extensional recovery and the determination of membrane viscosity. Biophys J. 1979 Apr;26(1):101–114. doi: 10.1016/S0006-3495(79)85238-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waugh R., Evans E. A. Thermoelasticity of red blood cell membrane. Biophys J. 1979 Apr;26(1):115–131. doi: 10.1016/S0006-3495(79)85239-X. [DOI] [PMC free article] [PubMed] [Google Scholar]