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. 1980 Feb;29(2):247–256. doi: 10.1016/S0006-3495(80)85129-0

Bathoproducts of rhodopsin, isorhodopsin I, and isorhodopsin II.

B Mao, T G Ebrey, R Crouch
PMCID: PMC1328694  PMID: 7260250

Abstract

Bathorhodopsins were prepared by partially (10--15%) photoconverting bovine rhodopsin (11-cis chromophore) or isorhodopsin I (9-cis chromophore) at 77 degrees K; care was taken to avoid establishing photostationary states. The absorption spectra calculated for the bathorhodopsins derived from the two parent pigments are identical in their lambda max 'S, bandwidths, and extinction coefficients. This result provides further support for the hypothesis that bathorhodopsin is a common intermediate between an 11-cis pigment (rhodopsin) and a 9-cis one (isorhodopsin I) and thus probably has an all-trans chromophore. This in turn is strong evidence for the cis-trans isomerization model of the primary event in vision. The spectrum of the bathoproduct of isorhodopsin II (9,13-dicis chromophore) is different from the other pigments' bathoproducts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crouch R., Purvin V., Nakanishi K., Ebrey T. Isorhodopsin II: artificial photosensitive pigment formed from 9,13-dicis retinal. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1538–1542. doi: 10.1073/pnas.72.4.1538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ebrey T. G. The use of Ammonyx LO in the purification of rhodopsin and rod outer segments. Vision Res. 1971 Sep;11(9):1007–1009. doi: 10.1016/0042-6989(71)90220-3. [DOI] [PubMed] [Google Scholar]
  3. Green B. H., Monger T. G., Alfano R. R., Aton B., Callender R. H. Cis-trans isomerisation in rhodopsin occurs in picoseconds. Nature. 1977 Sep 8;269(5624):179–180. doi: 10.1038/269179a0. [DOI] [PubMed] [Google Scholar]
  4. HUBBARD R. Retinene isomerase. J Gen Physiol. 1956 Jul 20;39(6):935–962. doi: 10.1085/jgp.39.6.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hurley J. B., Ebrey T. G., Honig B., Ottolenghi M. Temperature and wavelength effects on the photochemistry of rhodopsin, isorhodopsin, bacteriorhodopsin and their photoproducts. Nature. 1977 Dec 8;270(5637):540–542. doi: 10.1038/270540a0. [DOI] [PubMed] [Google Scholar]
  6. KROPF A., HUBBARD R. The mechanism of bleaching rhodopsin. Ann N Y Acad Sci. 1959 Nov 12;74(2):266–280. doi: 10.1111/j.1749-6632.1958.tb39550.x. [DOI] [PubMed] [Google Scholar]
  7. Mathies R., Oseroff A. R., Stryer L. Rapid-flow resonance Raman spectroscopy of photolabile molecules: rhodopsin and isorhodopsin. Proc Natl Acad Sci U S A. 1976 Jan;73(1):1–5. doi: 10.1073/pnas.73.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Monger T. G., Alfano R. R., Callender R. H. Photochemistry of rhodopsin and isorhodopsin investigated on a picosecond time scale. Biophys J. 1979 Jul;27(1):105–115. doi: 10.1016/S0006-3495(79)85205-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Oseroff A. R., Callender R. H. Resonance Raman spectroscopy of rhodopsin in retinal disk membranes. Biochemistry. 1974 Sep 24;13(20):4243–4248. doi: 10.1021/bi00717a027. [DOI] [PubMed] [Google Scholar]
  10. Peters K., Applebury M. L., Rentzepis P. M. Primary photochemical event in vision: proton translocation. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3119–3123. doi: 10.1073/pnas.74.8.3119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. WALD G., BROWN P. K. The molar extinction of rhodopsin. J Gen Physiol. 1953 Nov 20;37(2):189–200. doi: 10.1085/jgp.37.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. YOSHIZAWA T., WALD G. Pre-lumirhodopsin and the bleaching of visual pigments. Nature. 1963 Mar 30;197:1279–1286. doi: 10.1038/1971279a0. [DOI] [PubMed] [Google Scholar]

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