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. 1980 Jun;30(3):415–439. doi: 10.1016/S0006-3495(80)85105-8

Interactions of voltage-sensing dyes with membranes. I. Steady-state permeability behaviors induced by cyanine dyes.

S Krasne
PMCID: PMC1328748  PMID: 7260282

Abstract

The effects of a series of thiadicarbocyanine dyes, diSCn(5), in altering the electrical properties of lipid bilayer membranes have been studied as a function of the membrane's intrinsic surface-charge density, the aqueous ionic strength, and the length (n) of the hydrocarbon side chains on the dye. Zero-current conductances, transmembrane potentials, and conductance-voltage relationships induced by these dyes were measured. All dyes studied altered membrane permeability properties; however these alterations were much larger at lower (e.g. 10(-3) M) than at higher (e.g. 10(-1) M) ionic strengths. The data suggest that such perturbations would not be troublesome for most biological preparations in which these dyes have been studied. The mechanisms by which these dyes alter membrane permeabilities vary in going from short-chained to long-chained dyes, the former forming voltage-gated, ion-permeant pores and the latter acting predominantly as anion carriers (forming 2:1 dye-anion complexes). In the case of diSC3(5), the predominant mechanism of altering membrane permeabilities changes in going from neutral to negatively charged membranes and also depends upon aqueous ionic strength and dye concentration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen F. S., Eisenberg M., McLaughlin S. The kinetic mechanism of action of an uncoupler of oxidative phosphorylation. J Membr Biol. 1977 Dec 15;37(3-4):361–396. doi: 10.1007/BF01940940. [DOI] [PubMed] [Google Scholar]
  2. Cohen L. B., Salzberg B. M., Davila H. V., Ross W. N., Landowne D., Waggoner A. S., Wang C. H. Changes in axon fluorescence during activity: molecular probes of membrane potential. J Membr Biol. 1974;19(1):1–36. doi: 10.1007/BF01869968. [DOI] [PubMed] [Google Scholar]
  3. Cohen L. B., Salzberg B. M. Optical measurement of membrane potential. Rev Physiol Biochem Pharmacol. 1978;83:35–88. doi: 10.1007/3-540-08907-1_2. [DOI] [PubMed] [Google Scholar]
  4. Finkelstein A., Holz R. Aqueous pores created in thin lipid membranes by the polyene antibiotics nystatin and amphotericin B. Membranes. 1973;2:377–408. [PubMed] [Google Scholar]
  5. Foster M., McLaughlin S. Complexes between uncouplers of oxidative phosphorylation. J Membr Biol. 1974;17(2):155–180. doi: 10.1007/BF01870177. [DOI] [PubMed] [Google Scholar]
  6. Goldman D. E. POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES. J Gen Physiol. 1943 Sep 20;27(1):37–60. doi: 10.1085/jgp.27.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HODGKIN A. L., KATZ B. The effect of sodium ions on the electrical activity of giant axon of the squid. J Physiol. 1949 Mar 1;108(1):37–77. doi: 10.1113/jphysiol.1949.sp004310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haynes D. H., Simkowitz P. 1-Anilino-8-naphthalenesulfonate: a fluorescent probe of ion and ionophore transport kinetics and trans-membrane asymmetry. J Membr Biol. 1977 May 6;33(1-2):63–108. doi: 10.1007/BF01869512. [DOI] [PubMed] [Google Scholar]
  9. McLaughlin S. G., Szabo G., Eisenman G., Ciani S. M. Surface charge and the conductance of phospholipid membranes. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1268–1275. doi: 10.1073/pnas.67.3.1268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McLaughlin S. G., Szabo G., Eisenman G. Divalent ions and the surface potential of charged phospholipid membranes. J Gen Physiol. 1971 Dec;58(6):667–687. doi: 10.1085/jgp.58.6.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McLaughlin S., Harary H. The hydrophobic adsorption of charged molecules to bilayer membranes: a test of the applicability of the stern equation. Biochemistry. 1976 May 4;15(9):1941–1948. doi: 10.1021/bi00654a023. [DOI] [PubMed] [Google Scholar]
  12. McLaughlin S. The mechanism of action of DNP on phospholipid bilayer membranes. J Membr Biol. 1972;9(4):361–372. [PubMed] [Google Scholar]
  13. Neumcke B. The action of uncouplers on lipid bilayer membranes. Membranes. 1975;3:215–253. [PubMed] [Google Scholar]
  14. Smejtek P., Hsu K., Perman W. H. Electrical conductivity in lipid bilayer membranes induced by pentachlorophenol. Biophys J. 1976 Apr;16(4):319–336. doi: 10.1016/S0006-3495(76)85691-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Szabo G., Eisenman G., Laprade R., Ciani S. M., Krasne S. Experimentally observed effects of carriers on the electrical properties of bilayer membranes--equilibrium domain. With a contribution on the molecular basis of ion selectivity. Membranes. 1973;2:179–328. [PubMed] [Google Scholar]
  16. Waggoner A. S., Wang C. H., Tolles R. L. Mechanism of potential-dependent light absorption changes of lipid bilayer membranes in the presence of cyanine and oxonol dyes. J Membr Biol. 1977 May 6;33(1-2):109–140. doi: 10.1007/BF01869513. [DOI] [PubMed] [Google Scholar]
  17. Waggoner A. Optical probes of membrane potential. J Membr Biol. 1976 Jun 30;27(4):317–334. doi: 10.1007/BF01869143. [DOI] [PubMed] [Google Scholar]
  18. White S. H. A study of lipid bilayer membrane stability using precise measurements of specific capacitance. Biophys J. 1970 Dec;10(12):1127–1148. doi: 10.1016/S0006-3495(70)86360-3. [DOI] [PMC free article] [PubMed] [Google Scholar]

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