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. 1980 Jul;31(1):31–43. doi: 10.1016/S0006-3495(80)85038-7

Nitella fluctuation and instability in the membrane potential near threshold.

H Hayashi, K Hirakawa
PMCID: PMC1328762  PMID: 7272431

Abstract

Excitation of Nitella internodal cell was investigated as an example of the phase transition in an open system far more thermal equilibrium. The power density spectrum of the membrane potential fluctuation had a bulge in a frequency range lower than 1 Hz at the resting state and a peak at approximately 0.03 Hz at a depolarized state near the threshold. A critical oscillation in the membrane potential was observed when threshold was gradually approached from the resting state. Repetitive firing was observed under a step-current of the superthreshold value. The frequency of spectral peaking, critical oscillation, and repetitive firing agree well with each other. The result suggests that the hard-mode instability occurs in the Nitella internodal cell. The membrane impedance had no peak in the same frequency region as the peak of the voltage spectrum. The spectral peak may be ascribed to be electrogenic pump modulated by the metabolic feedback system in photosynthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adelman W. J., Jr, Fitzhugh R. Solutions of the Hodgkin-Huxley equations modified for potassium accumulation in a periaxonal space. Fed Proc. 1975 Apr;34(5):1322–1329. [PubMed] [Google Scholar]
  2. Chen Yi-der Fluctuations and noise in kinetic systems. III. Cycling steady-state models. J Theor Biol. 1975 Nov;55(1):229–243. doi: 10.1016/s0022-5193(75)80117-2. [DOI] [PubMed] [Google Scholar]
  3. Chen Y. D. Differentiation between equilibrium and nonequilibrium kinetic systems by noise analysis. Biophys J. 1978 Mar;21(3):279–285. doi: 10.1016/S0006-3495(78)85525-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen Y. D., Hill T. L. Fluctuations and noise in kinetic systems. Application to K+ channels in the squid axon. Biophys J. 1973 Dec;13(12):1276–1295. doi: 10.1016/S0006-3495(73)86062-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DeFelice L. J., Wanke E., Conti F. Potassium and sodium current noise from squid axon membranes. Fed Proc. 1975 Apr;34(5):1338–1342. [PubMed] [Google Scholar]
  6. Ferrier J. M., Morvan C. Plasmalemma Voltage Noise in Chara corallina. Plant Physiol. 1979 Apr;63(4):709–714. doi: 10.1104/pp.63.4.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fishman H. M., Moore L. E., Poussart D. M. Potassium-ion conduction noise in squid axon membrane. J Membr Biol. 1975 Dec 4;24(3-4):305–328. doi: 10.1007/BF01868629. [DOI] [PubMed] [Google Scholar]
  8. Fishman H. M., Poussart D. M., Moore L. E. Noise measurements in squid axon membrane. J Membr Biol. 1975 Dec 4;24(3-4):281–304. doi: 10.1007/BF01868628. [DOI] [PubMed] [Google Scholar]
  9. Fishman H. M. Relaxation spectra of potassium channel noise from squid axon membranes. Proc Natl Acad Sci U S A. 1973 Mar;70(3):876–879. doi: 10.1073/pnas.70.3.876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GAFFEY C. T., MULLINS L. J. Ion fluxes during the action potential in Chara. J Physiol. 1958 Dec 30;144(3):505–524. doi: 10.1113/jphysiol.1958.sp006116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guttman R., Barnhill R. Oscillation and repetitive firing in squid axons. Comparison of experiments with computations. J Gen Physiol. 1970 Jan;55(1):104–118. doi: 10.1085/jgp.55.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HAGIWARA S., OOMURA Y. The critical depolarization for the spike in the squid giant axon. Jpn J Physiol. 1958 Sep 15;8(3):234–245. doi: 10.2170/jjphysiol.8.234. [DOI] [PubMed] [Google Scholar]
  13. HODGKIN A. L., HUXLEY A. F. A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol. 1952 Aug;117(4):500–544. doi: 10.1113/jphysiol.1952.sp004764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hill T. L., Chen Y. D. On the theory of ion transport across the nerve membrane. IV. Noise from the open-close kinetics of K + channels. Biophys J. 1972 Aug;12(8):948–959. doi: 10.1016/S0006-3495(72)86136-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Inoue I., Kobatake Y., Tasaki I. Excitability, instability and phase transitions in squid axon membrane under internal perfusion with dilute salt solutions. Biochim Biophys Acta. 1973 May 25;307(3):471–477. doi: 10.1016/0005-2736(73)90294-0. [DOI] [PubMed] [Google Scholar]
  16. KISHIMOTO U. CURRENT VOLTAGE RELATIONS IN NITELLA. Jpn J Physiol. 1964 Oct 15;14:515–527. doi: 10.2170/jjphysiol.14.515. [DOI] [PubMed] [Google Scholar]
  17. Kitasato H. The influence of H+ on the membrane potential and ion fluxes of Nitella. J Gen Physiol. 1968 Jul;52(1):60–87. doi: 10.1085/jgp.52.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kobatake Y., Inoue I., Ueda T. Physical chemistry of excitable membranes. Adv Biophys. 1975;7:43–89. [PubMed] [Google Scholar]
  19. MULLINS L. J. Efflux of chloride ions during the action potential of Nitella. Nature. 1962 Dec 8;196:986–987. doi: 10.1038/196986a0. [DOI] [PubMed] [Google Scholar]
  20. Mailman D. S., Mullins L. J. The electrical measurement of chloride fluxes in Nitella. Aust J Biol Sci. 1966 Jun;19(3):385–398. doi: 10.1071/bi9660385. [DOI] [PubMed] [Google Scholar]
  21. Spanswick R. M. Evidence for an electrogenic ion pump in Nitella translucens. I. The effects of pH, K + , Na + , light and temperature on the membrane potential and resistance. Biochim Biophys Acta. 1972 Oct 23;288(1):73–89. doi: 10.1016/0005-2736(72)90224-6. [DOI] [PubMed] [Google Scholar]
  22. Stevens C. F. Inferences about membrane properties from electrical noise measurements. Biophys J. 1972 Aug;12(8):1028–1047. doi: 10.1016/S0006-3495(72)86141-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vredenberg W. J., Tonk W. J. Photosynthetic energy control of an electrogenic ion pump at the plasmalemma of Nitella translucens. Biochim Biophys Acta. 1973 Mar 16;298(2):354–368. doi: 10.1016/0005-2736(73)90364-7. [DOI] [PubMed] [Google Scholar]

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