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. 1980 Aug;31(2):215–228. doi: 10.1016/S0006-3495(80)85052-1

Dielectric analysis of Escherichia coli suspensions in the light of the theory of interfacial polarization.

K Asami, T Hanai, N Koizumi
PMCID: PMC1328779  PMID: 7020783

Abstract

Dielectric measurements of Escherichia coli suspensions were carried out over a frequency range from 10 kHz to 100 MHz, and marked dielectric dispersions having characteristic frequency of approximately 1 MHz were observed. On the basis of the cell model that a spheroid is covered with two confocal shells, a dielectric theory was developed to determine accurately four electrical parameters for E. coli cells such as the conductivity of the cell wall, the dielectric constant of the cell membrane, and the dielectric constant and the conductivity of the protoplasm. The observed data were analyzed by means of the procedure based on the dielectric theory to yield a set of plausible electrical parameters for the cells. By taking account of the size distribution of the cells and a dielectric relaxation of the protoplasm, the observed dispersion curves were successfully reconstituted by the present theory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asami K., Hanai T., Koizumi N. Dielectric properties of yeast cells. J Membr Biol. 1976 Aug 26;28(2-3):169–180. doi: 10.1007/BF01869695. [DOI] [PubMed] [Google Scholar]
  2. Carstensen E. L., Cox H. A., Mercer W. B., Natale L. A. Passive Electrical Properties of Microorganisms: I. Conductivity of Escherichia coli and Micrococcus lysodeikticus. Biophys J. 1965 May;5(3):289–300. doi: 10.1016/s0006-3495(65)86717-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carstensen E. L. Passive electrical properties of microorganisms. II. Resistance of the bacterial membrane. Biophys J. 1967 Sep;7(5):493–503. doi: 10.1016/S0006-3495(67)86600-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Einolf C. W., Jr, Carstensen E. L. Passive electrical properties of microorganisms. IV. Studies of the protoplasts of Micrococcus lysodeikticus. Biophys J. 1969 Apr;9(4):634–643. doi: 10.1016/S0006-3495(69)86408-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRICKE H., SCHWAN H. P., LI K., BRYSON V. A dielectric study of the low-conductance surface membrane in E. coli. Nature. 1956 Jan 21;177(4499):134–135. doi: 10.1038/177134a0. [DOI] [PubMed] [Google Scholar]
  6. Hanai T., Koizumi N., Irimajiri A. A method for determining the dielectric constant and the conductivity of membrane-bounded particles of biological relevance. Biophys Struct Mech. 1975 Dec 19;1(4):285–294. doi: 10.1007/BF00537642. [DOI] [PubMed] [Google Scholar]
  7. Irimajiri A., Doida Y., Hanai T., Inouye A. Passive electrical properties of cultured murine lymphoblast (L5178Y) with reference to its cytoplasmic membrane, nuclear envelope, and intracellular phases. J Membr Biol. 1978 Jan 18;38(3):209–232. doi: 10.1007/BF01871923. [DOI] [PubMed] [Google Scholar]
  8. Irimajiri A., Hanai T., Inouye A. A dielectric theory of "multi-stratified shell" model with its application to a lymphoma cell. J Theor Biol. 1979 May 21;78(2):251–269. doi: 10.1016/0022-5193(79)90268-6. [DOI] [PubMed] [Google Scholar]
  9. Irimajiri A., Hanai T., Inouye A. Dielectric properties of synaptosomes isolated from rat brain cortex. Biophys Struct Mech. 1975 Dec 19;1(4):273–283. doi: 10.1007/BF00537641. [DOI] [PubMed] [Google Scholar]
  10. PAULY H., SCHWAN H. P. Uber die Impedanz einer Suspension von kugelförmigen Teilchen mit einer Schale; Ein Modell fur das dielektrische Verhalten von Zellsuspensionen und von Proteinlösungen. Z Naturforsch B. 1959 Feb;14B(2):125–131. [PubMed] [Google Scholar]
  11. SCHWAN H. P., MOROWITZ H. J. Electrical properties of the membranes of the pleuropneumonia-like organism A 5969. Biophys J. 1962 Sep;2:395–407. doi: 10.1016/s0006-3495(62)86863-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Takashima S., Schwan H. P. Passive electrical properties of squid axon membrane. J Membr Biol. 1974;17(1):51–68. doi: 10.1007/BF01870172. [DOI] [PubMed] [Google Scholar]
  13. van Iterson W. Symposium on the fine structure and replication of bacteria and their parts. II. Bacterial cytoplasm. Bacteriol Rev. 1965 Sep;29(3):299–325. doi: 10.1128/br.29.3.299-325.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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