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. 1980 Sep;31(3):359–370. doi: 10.1016/S0006-3495(80)85064-8

Statistical mechanical analysis of Raman spectroscopic order parameter changes in pressure-induced lipid bilayer phase transitions.

P Yager, W L Peticolas
PMCID: PMC1328795  PMID: 6894876

Abstract

The statistical mechanical cluster theory of Fisher as applied by Kanehisa and Tsong to phospholipid bilayers is modified to describe the effects of hydrostatic pressure on the state of an aqueous dispersion of the phospholipid dipalmitoyl phosphatidylcholine. A high pressure Raman scattering cell has been built to obtain the Raman spectra of aqueous dispersions of phospholipids as a function of the applied hydrostatic pressure from 0 to 100 atmospheres. Predicted thermal and pressure-induced phase transitions are compared with an experimentally obtained Raman order parameter derived from the ratio of two bands in the C-H stretching region of the Raman spectrum of the sample. The parameters of the theory are adjusted to obtain a satisfactory fit of the Raman order parameter versus temperature. The theory is then found to give an excellent prediction of the observed pressure dependence of the Raman order parameter with no changes in the adjustable parameters. The implications of the success of the theoretical fit is discussed. Particularly of interest is the rather high value of the critical temperature, Tc, for lipid bilayers which is predicted by the model.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albon N., Sturtevant J. M. Nature of the gel to liquid crystal transition of synthetic phosphatidylcholines. Proc Natl Acad Sci U S A. 1978 May;75(5):2258–2260. doi: 10.1073/pnas.75.5.2258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gaber B. P., Peticolas W. L. On the quantitative interpretation of biomembrane structure by Raman spectroscopy. Biochim Biophys Acta. 1977 Mar 1;465(2):260–274. doi: 10.1016/0005-2736(77)90078-5. [DOI] [PubMed] [Google Scholar]
  3. Gaber B. P., Yager P., Peticolas W. L. Conformational nonequivalence of chains 1 and 2 of dipalmitoyl phosphatidylcholine as observed by Raman spectroscopy. Biophys J. 1978 Dec;24(3):677–688. doi: 10.1016/S0006-3495(78)85413-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gaber B. P., Yager P., Peticolas W. L. Deuterated phospholipids as nonperturbing components for Raman studies of biomembranes. Biophys J. 1978 May;22(2):191–207. doi: 10.1016/S0006-3495(78)85484-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gaber B. P., Yager P., Peticolas W. L. Interpretation of biomembrane structure by Raman difference spectroscopy. Nature of the endothermic transitions in phosphatidylcholines. Biophys J. 1978 Feb;21(2):161–176. doi: 10.1016/S0006-3495(78)85516-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hui S. W., Cowden M., Papahadjopoulos D., Parsons D. F. Electron diffraction study of hydrated phospholipid single bilayers. Effects of temperature hydration and surface pressure of the "precursor" monolayer. Biochim Biophys Acta. 1975 Mar 25;382(3):265–275. doi: 10.1016/0005-2736(75)90269-2. [DOI] [PubMed] [Google Scholar]
  7. Kamaya H., Ueda I., Moore P. S., Eyring H. Antagonism between high pressure and anesthetics in the thermal phase-transition of dipalmitoyl phosphatidylcholine bilayer. Biochim Biophys Acta. 1979 Jan 5;550(1):131–137. doi: 10.1016/0005-2736(79)90121-4. [DOI] [PubMed] [Google Scholar]
  8. Luna E. J., McConnell H. M. The intermediate monoclinic phase of phosphatidylcholines. Biochim Biophys Acta. 1977 May 2;466(3):381–392. doi: 10.1016/0005-2736(77)90331-5. [DOI] [PubMed] [Google Scholar]
  9. Macdonald A. G. A dilatometric investigation of the effects of general anaesthetics, alcohols and hydrostatic pressure on the phase transition in smectic mesophases of dipalmitoyl phosphatidylcholine. Biochim Biophys Acta. 1978 Feb 2;507(1):26–37. doi: 10.1016/0005-2736(78)90371-1. [DOI] [PubMed] [Google Scholar]
  10. Mendelsohn R., Maisano J. Use of deuterated phospholipids in Raman spectroscopic studies of membrane structure. I. Multilayers of dimyristoyl phosphatidylcholine (and its -d54 derivative) with distearoyl phosphatidylcholine. Biochim Biophys Acta. 1978 Jan 19;506(2):192–201. doi: 10.1016/0005-2736(78)90390-5. [DOI] [PubMed] [Google Scholar]
  11. Nagle J. F., Wilkinson D. A. Lecithin bilayers. Density measurement and molecular interactions. Biophys J. 1978 Aug;23(2):159–175. doi: 10.1016/S0006-3495(78)85441-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Phillips M. C., Chapman D. Monolayer characteristics of saturated 1,2,-diacyl phosphatidylcholines (lecithins) and phosphatidylethanolamines at the air-water interface. Biochim Biophys Acta. 1968 Nov 5;163(3):301–313. doi: 10.1016/0005-2736(68)90115-6. [DOI] [PubMed] [Google Scholar]
  13. Wallach D. F., Verma S. P., Fookson J. Application of laser Raman and infrared spectroscopy to the analysis of membrane structure. Biochim Biophys Acta. 1979 Aug 20;559(2-3):153–208. doi: 10.1016/0304-4157(79)90001-7. [DOI] [PubMed] [Google Scholar]
  14. Wilkinson D. A., Nagle J. F. A differential dilatometer. Anal Biochem. 1978 Jan;84(1):263–271. doi: 10.1016/0003-2697(78)90509-2. [DOI] [PubMed] [Google Scholar]
  15. Yellin N., Levin I. W. Hydrocarbon chain disorder in lipid bilayers. Temperature dependent Raman spectra of 1,2-diacyl phosphatidylcholine-water gels. Biochim Biophys Acta. 1977 Nov 24;489(2):177–190. doi: 10.1016/0005-2760(77)90137-0. [DOI] [PubMed] [Google Scholar]

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