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. 1982 Mar;37(3):633–636.

Lipid-protein interactions in sarcoplasmic reticulum are not perturbed by ionophore A23187. An EPR and fluorescence study.

M J Pringle, C Hidalgo
PMCID: PMC1328848  PMID: 6280784

Abstract

The divalent-cation ionophore A23187 at micromolar concentrations prevents the ATP-dependent accumulation of calcium into sarcoplasmic reticulum vesicles. Under the same conditions and throughout the temperature range of 4 degrees-37 degrees C, A23187 has no effect on either the rotational motion of the Ca2+ -ATPase in the membrane, or on the mobility of the lipid acyl chains. The steady-state fluorescence polarization of a polyene fluorescent probe incorporated into the membrane lipids was similarly unaffected by the ionophore. These results show conclusively that the mechanism of action of the ionophore does not involve significant of lipid-protein interactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Case G. D., Vanderkooi J. M., Scarpa A. Physical properties of biological membranes determined by the fluorescence of the calcium ionophore A23187. Arch Biochem Biophys. 1974 May;162(1):174–185. doi: 10.1016/0003-9861(74)90116-7. [DOI] [PubMed] [Google Scholar]
  2. Caswell A. H., Pressman B. C. Kinetics of transport of divalent cations across sarcoplasmic reticulum vesicles induced by ionophores. Biochem Biophys Res Commun. 1972 Oct 6;49(1):292–298. doi: 10.1016/0006-291x(72)90043-5. [DOI] [PubMed] [Google Scholar]
  3. Fernandez J. L., Rosemblatt M., Hidalgo C. Highly purified sarcoplasmic reticulum vesicles are devoid of Ca2+-independent ('basal') ATPase activity. Biochim Biophys Acta. 1980 Jul;599(2):552–568. doi: 10.1016/0005-2736(80)90199-6. [DOI] [PubMed] [Google Scholar]
  4. Heyn M. P., Cherry R. J., Dencher N. A. Lipid--protein interactions in bacteriorhodopsin--dimyristoylphosphatidylcholine vesicles. Biochemistry. 1981 Feb 17;20(4):840–849. doi: 10.1021/bi00507a029. [DOI] [PubMed] [Google Scholar]
  5. Hidalgo C., Ikemoto N., Gergely J. Role of phospholipids in the calcium-dependent ATPase of the sarcoplasmic reticulum. Enzymatic and ESR studies with phospholipid-replaced membranes. J Biol Chem. 1976 Jul 25;251(14):4224–4232. [PubMed] [Google Scholar]
  6. Hidalgo C. Inhibition of calcium transport in sarcoplasmic reticulum after modification of highly reactive amino groups. Biochem Biophys Res Commun. 1980 Feb 12;92(3):757–765. doi: 10.1016/0006-291x(80)90768-8. [DOI] [PubMed] [Google Scholar]
  7. Hidalgo C., Thomas D. D., Ikemoto N. Effect of the lipid environment on protein motion and enzymatic activity of sarcoplasmic reticulum calcium ATPase. J Biol Chem. 1978 Oct 10;253(19):6879–6887. [PubMed] [Google Scholar]
  8. Hubbell W. L., McConnell H. M. Molecular motion in spin-labeled phospholipids and membranes. J Am Chem Soc. 1971 Jan 27;93(2):314–326. doi: 10.1021/ja00731a005. [DOI] [PubMed] [Google Scholar]
  9. Kinosita K., Jr, Kataoka R., Kimura Y., Gotoh O., Ikegami A. Dynamic structure of biological membranes as probed by 1,6-diphenyl-1,3,5-hexatriene: a nanosecond fluorescence depolarization study. Biochemistry. 1981 Jul 21;20(15):4270–4277. doi: 10.1021/bi00518a006. [DOI] [PubMed] [Google Scholar]
  10. Pfeiffer D. R., Reed P. W., Lardy H. A. Ultraviolet and fluorescent spectral properties of the divalent cation ionophore A23187 and its metal ion complexes. Biochemistry. 1974 Sep 10;13(19):4007–4014. doi: 10.1021/bi00716a029. [DOI] [PubMed] [Google Scholar]
  11. Reed P. W., Lardy H. A. A23187: a divalent cation ionophore. J Biol Chem. 1972 Nov 10;247(21):6970–6977. [PubMed] [Google Scholar]
  12. Shimshick E. J., McConnell H. M. Lateral phase separation in phospholipid membranes. Biochemistry. 1973 Jun 5;12(12):2351–2360. doi: 10.1021/bi00736a026. [DOI] [PubMed] [Google Scholar]
  13. Shinitzky M., Barenholz Y. Dynamics of the hydrocarbon layer in liposomes of lecithin and sphingomyelin containing dicetylphosphate. J Biol Chem. 1974 Apr 25;249(8):2652–2657. [PubMed] [Google Scholar]
  14. Thomas D. D., Bigelow D. J., Squier T. C., Hidalgo C. Rotational dynamics of protein and boundary lipid in sarcoplasmic reticulum membrane. Biophys J. 1982 Jan;37(1):217–225. doi: 10.1016/S0006-3495(82)84671-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thomas D. D., Hidalgo C. Rotational motion of the sarcoplasmic reticulum Ca2+-ATPase. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5488–5492. doi: 10.1073/pnas.75.11.5488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolber P. K., Hudson B. S. Fluorescence lifetime and time-resolved polarization anisotropy studies of acyl chain order and dynamics in lipid bilayers. Biochemistry. 1981 May 12;20(10):2800–2810. doi: 10.1021/bi00513a015. [DOI] [PubMed] [Google Scholar]

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