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. 1982 Jun;38(3):323–326. doi: 10.1016/S0006-3495(82)84566-9

Direct High-resolution Nuclear Magnetic Resonance Studies of Cation Transport in Vivo

Na+ Transport in Yeast Cells

James A Balschi, Vincent P Cirillo, Charles S Springer Jr
PMCID: PMC1328876  PMID: 7049258

Abstract

A new nuclear magnetic resonance (NMR) method for monitoring transmembrane metal cation transport is reported. It is illustrated with a study of Na+ efflux from Na+-rich yeast cells. The technique involves the use of an anionic paramagnetic shift reagent, present only outside the cells, to induce a splitting of the sodium-23 NMR peak, in this case, into components representing intra- and extracellular Na+. The time course of the efflux is in good agreement with the literature and can be well fitted with a double exponential decay expression. Splitting of the lithium-7 NMR signal from a suspension of Li+-rich respiratory-deficient, petite yeasts is also reported.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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