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. 1983 Feb;41(2):223–226. doi: 10.1016/S0006-3495(83)84423-3

Charge Movement in a Fast Twitch Skeletal Muscle from Rat

B J Simon, K G Beam
PMCID: PMC1329170  PMID: 6838965

Abstract

Voltage-dependent charge movement in the rat omohyoid muscle was investigated using the three microelectrode voltage clamp technique. The charge that moved during a depolarization from the holding potential (-90 mV) to the test potential, V, increased with increasing V, saturating around 0 mV. The charge vs. voltage relationship was well fitted by Q = Qmax/{1 + exp[-(V - V)/k]}, with Qmax = 28.5 nC/μF, V = -34.2 mV, and k = 8.7 mV. Repolarization of the fiber from the test potential back to the holding potential caused an equal but opposite amount of charge to move. The kinetics of ON charge movement could be well described by a model developed for frog muscle by Horowicz and Schneider (1981b), which suggests that rat and frog charge movements are similar. This model failed to describe the kinetics of OFF charge movement for steps in potential from 0 mV to test potentials of -10 to -90 mV. OFF-charge movement rose to a peak more slowly and decayed more slowly than predicted by the theory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adrian R. H., Almers W. Charge movement in the membrane of striated muscle. J Physiol. 1976 Jan;254(2):339–360. doi: 10.1113/jphysiol.1976.sp011235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adrian R. H., Chandler W. K., Hodgkin A. L. Voltage clamp experiments in striated muscle fibres. J Physiol. 1970 Jul;208(3):607–644. doi: 10.1113/jphysiol.1970.sp009139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adrian R. H., Peachey L. D. Reconstruction of the action potential of frog sartorius muscle. J Physiol. 1973 Nov;235(1):103–131. doi: 10.1113/jphysiol.1973.sp010380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandler W. K., Rakowski R. F., Schneider M. F. A non-linear voltage dependent charge movement in frog skeletal muscle. J Physiol. 1976 Jan;254(2):245–283. doi: 10.1113/jphysiol.1976.sp011232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dulhunty A. F. Potassium contractures and mechanical activation in mammalian skeletal muscles. J Membr Biol. 1980 Dec 30;57(3):223–233. doi: 10.1007/BF01869590. [DOI] [PubMed] [Google Scholar]
  6. Gilly W. F., Hui C. S. Voltage-dependent charge movement in frog slow muscle fibres. J Physiol. 1980 Apr;301:175–190. doi: 10.1113/jphysiol.1980.sp013197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hollingworth S., Marshall M. W. A comparative study of charge movement in rat and frog skeletal muscle fibres. J Physiol. 1981 Dec;321:583–602. doi: 10.1113/jphysiol.1981.sp014004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Horowicz P., Schneider M. F. Membrane charge moved at contraction thresholds in skeletal muscle fibres. J Physiol. 1981 May;314:595–633. doi: 10.1113/jphysiol.1981.sp013726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Horowicz P., Schneider M. F. Membrane charge movement in contracting and non-contracting skeletal muscle fibres. J Physiol. 1981 May;314:565–593. doi: 10.1113/jphysiol.1981.sp013725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huang C. L. Pharmacological separation of charge movement components in frog skeletal muscle. J Physiol. 1982 Mar;324:375–387. doi: 10.1113/jphysiol.1982.sp014118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hui C. S. Pharmacological dissection of charge movement in frog skeletal muscle fibers. Biophys J. 1982 Jul;39(1):119–122. doi: 10.1016/S0006-3495(82)84498-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kovács L., Ríos E., Schneider M. F. Calcium transients and intramembrane charge movement in skeletal muscle fibres. Nature. 1979 May 31;279(5712):391–396. doi: 10.1038/279391a0. [DOI] [PubMed] [Google Scholar]
  13. Müntener M., Gottschall J., Neuhuber W., Mysicka A., Zenker W. The ansa cervicalis and the infrahyoid muscles of the rat. I. Anatomy; distribution, number and diameter of fiber types; motor units. Anat Embryol (Berl) 1980;159(1):49–57. doi: 10.1007/BF00299254. [DOI] [PubMed] [Google Scholar]
  14. Palade P. T., Barchi R. L. Characteristics of the chloride conductance in muscle fibers of the rat diaphragm. J Gen Physiol. 1977 Mar;69(3):325–342. doi: 10.1085/jgp.69.3.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schneider M. F., Chandler W. K. Effects of membrane potential on the capacitance of skeletal muscle fibers. J Gen Physiol. 1976 Feb;67(2):125–163. doi: 10.1085/jgp.67.2.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shlevin H. H. Effects of external calcium concentration and pH on charge movement in frog skeletal muscle. J Physiol. 1979 Mar;288:129–158. [PMC free article] [PubMed] [Google Scholar]

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