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. 1983 Jun;42(3):285–293. doi: 10.1016/S0006-3495(83)84396-3

1H-NMR study of the three low temperature phases of DPPC-water systems.

L Trahms, W D Klabe, E Boroske
PMCID: PMC1329237  PMID: 6688190

Abstract

The three phases of dipalmitoylphosphatidylcholine-water dispersions, occurring below the main transition are studied by a moment analysis of 1H-nuclear magnetic resonance (NMR) spectra. The subtransition, recently detected by Chen, S. C., J. M. Sturtevant, and B. J. Gaffney, 1980, Pro. Natl. Acad. Sci. USA, 77:5060-5063, is characterized by a sharp drop in the second moment at 12 degrees C as a result of increasing the temperature. Interesting features of this phase transition are a hysteresis of 11 K and extremely slow kinetics. It is interpreted as the onset of a flip-flop of the hydrocarbon chains about their long axis. At the pretransition, this type of motion is assumed to change into a fast rotation. The proposed models for the three phases are confirmed by computer calculations of theoretical values for the second and fourth moments of the corresponding NMR signals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloom M., Burnell E. E., MacKay A. L., Nichol C. P., Valic M. I., Weeks G. Fatty acyl chain order in lecithin model membranes determined from proton magnetic resonance. Biochemistry. 1978 Dec 26;17(26):5750–5762. doi: 10.1021/bi00619a024. [DOI] [PubMed] [Google Scholar]
  2. Boroske E., Trahms L. A 1H and 13C NMR study of motional changes of dipalmitoyl lecithin associated with the pretransition. Biophys J. 1983 Jun;42(3):275–283. doi: 10.1016/S0006-3495(83)84395-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brady G. W., Fein D. B. An analysis of the X-ray interchain peak profile in dipalmitoylglycerophosphocholine. Biochim Biophys Acta. 1977 Jan 21;464(2):249–259. doi: 10.1016/0005-2736(77)90001-3. [DOI] [PubMed] [Google Scholar]
  4. Büldt G., Gally H. U., Seelig A., Seelig J., Zaccai G. Neutron diffraction studies on selectively deuterated phospholipid bilayers. Nature. 1978 Jan 12;271(5641):182–184. doi: 10.1038/271182a0. [DOI] [PubMed] [Google Scholar]
  5. Büldt G., Wohlgemuth R. The headgroup conformation of phospholipids in membranes. J Membr Biol. 1981 Feb 15;58(2):81–100. doi: 10.1007/BF01870972. [DOI] [PubMed] [Google Scholar]
  6. Cameron D. G., Casal H. L., Gudgin E. F., Mantsch H. H. The gel phase of dipalmitoyl phosphatidylcholine. An infrared characterization of the acyl chain packing. Biochim Biophys Acta. 1980 Mar 13;596(3):463–467. doi: 10.1016/0005-2736(80)90135-2. [DOI] [PubMed] [Google Scholar]
  7. Cameron D. G., Casal H. L., Mantsch H. H., Boulanger Y., Smith I. C. The thermotropic behavior of dipalmitoyl phosphatidylcholine bilayers. A Fourier transform infrared study of specifically labeled lipids. Biophys J. 1981 Jul;35(1):1–16. doi: 10.1016/S0006-3495(81)84769-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cameron D. G., Casal H. L., Mantsch H. H. Characterization of the pretransition in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine by Fourier transform infrared spectroscopy. Biochemistry. 1980 Aug 5;19(16):3665–3672. doi: 10.1021/bi00557a005. [DOI] [PubMed] [Google Scholar]
  9. Chen S. C., Sturtevant J. M., Gaffney B. J. Scanning calorimetric evidence for a third phase transition in phosphatidylcholine bilayers. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5060–5063. doi: 10.1073/pnas.77.9.5060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Copeland B. R., McConnel H. M. The rippled structure in bilayer membranes of phosphatidylcholine and binary mixtures of phosphatidylcholine and cholesterol. Biochim Biophys Acta. 1980 Jun 20;599(1):95–109. doi: 10.1016/0005-2736(80)90059-0. [DOI] [PubMed] [Google Scholar]
  11. Cullis P. R. Lateral diffusion rates of phosphatidylcholine in vesicle membranes: effects of cholesterol and hydrocarbon phase transitions. FEBS Lett. 1976 Nov;70(1):223–228. doi: 10.1016/0014-5793(76)80762-4. [DOI] [PubMed] [Google Scholar]
  12. Davis J. H. Deuterium magnetic resonance study of the gel and liquid crystalline phases of dipalmitoyl phosphatidylcholine. Biophys J. 1979 Sep;27(3):339–358. doi: 10.1016/S0006-3495(79)85222-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Füldner H. H. Characterization of a third phase transition in multilamellar dipalmitoyllecithin liposomes. Biochemistry. 1981 Sep 29;20(20):5707–5710. doi: 10.1021/bi00523a011. [DOI] [PubMed] [Google Scholar]
  14. Gaber B. P., Peticolas W. L. On the quantitative interpretation of biomembrane structure by Raman spectroscopy. Biochim Biophys Acta. 1977 Mar 1;465(2):260–274. doi: 10.1016/0005-2736(77)90078-5. [DOI] [PubMed] [Google Scholar]
  15. Gebhardt C., Gruler H., Sackmann E. On domain structure and local curvature in lipid bilayers and biological membranes. Z Naturforsch C. 1977 Jul-Aug;32(7-8):581–596. doi: 10.1515/znc-1977-7-817. [DOI] [PubMed] [Google Scholar]
  16. Janiak M. J., Small D. M., Shipley G. G. Nature of the Thermal pretransition of synthetic phospholipids: dimyristolyl- and dipalmitoyllecithin. Biochemistry. 1976 Oct 19;15(21):4575–4580. doi: 10.1021/bi00666a005. [DOI] [PubMed] [Google Scholar]
  17. Janiak M. J., Small D. M., Shipley G. G. Temperature and compositional dependence of the structure of hydrated dimyristoyl lecithin. J Biol Chem. 1979 Jul 10;254(13):6068–6078. [PubMed] [Google Scholar]
  18. Kohler S. J., Klein M. P. Orientation and dynamics of phospholipid head groups in bilayers and membranes determined from 31P nuclear magnetic resonance chemical shielding tensors. Biochemistry. 1977 Feb 8;16(3):519–526. doi: 10.1021/bi00622a028. [DOI] [PubMed] [Google Scholar]
  19. Luna E. J., McConnell H. M. The intermediate monoclinic phase of phosphatidylcholines. Biochim Biophys Acta. 1977 May 2;466(3):381–392. doi: 10.1016/0005-2736(77)90331-5. [DOI] [PubMed] [Google Scholar]
  20. MacKay A. L. A proton NMR moment study of the gel and liquid-crystalline phases of dipalmitoyl phosphatidylcholine. Biophys J. 1981 Aug;35(2):301–313. doi: 10.1016/S0006-3495(81)84791-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Peters A., Kimmich R. The heterogenious solubility of oxygen in aqeuous lecithin dispersions and its relation to chain mobility. A NMR relaxation and wide-line study. Biophys Struct Mech. 1977 Dec 27;4(1):67–85. [PubMed] [Google Scholar]
  22. Pink D. A., Green T. J., Chapman D. Raman scattering in bilayers of saturated phosphatidylcholines. Experiment and theory. Biochemistry. 1980 Jan 22;19(2):349–356. doi: 10.1021/bi00543a016. [DOI] [PubMed] [Google Scholar]
  23. Rand R. P., Chapman D., Larsson K. Tilted hydrocarbon chains of dipalmitoyl lecithin become perpendicular to the bilayer before melting. Biophys J. 1975 Nov;15(11):1117–1124. doi: 10.1016/S0006-3495(75)85888-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Seelig A., Seelig J. The dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonance. Biochemistry. 1974 Nov 5;13(23):4839–4845. doi: 10.1021/bi00720a024. [DOI] [PubMed] [Google Scholar]
  25. Seelig J. 31P nuclear magnetic resonance and the head group structure of phospholipids in membranes. Biochim Biophys Acta. 1978 Jul 31;515(2):105–140. doi: 10.1016/0304-4157(78)90001-1. [DOI] [PubMed] [Google Scholar]
  26. Seelig J., Gally G. U., Wohlgemuth R. Orientation and flexibility of the choline head group in phosphatidylcholine bilayers. Biochim Biophys Acta. 1977 Jun 2;467(2):109–119. doi: 10.1016/0005-2736(77)90188-2. [DOI] [PubMed] [Google Scholar]
  27. Seelig J., Niederberger W. Two pictures of a lipid bilayer. A comparison between deuterium label and spin-label experiments. Biochemistry. 1974 Apr 9;13(8):1585–1588. doi: 10.1021/bi00705a005. [DOI] [PubMed] [Google Scholar]
  28. Smith B. A., McConnell H. M. Determination of molecular motion in membranes using periodic pattern photobleaching. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759–2763. doi: 10.1073/pnas.75.6.2759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tardieu A., Luzzati V., Reman F. C. Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases. J Mol Biol. 1973 Apr 25;75(4):711–733. doi: 10.1016/0022-2836(73)90303-3. [DOI] [PubMed] [Google Scholar]
  30. Trahms L., Boroske E. Pulse NMR study of phase transitions in dipalmitoyl phosphatidylcholine multilayer systems. Biochim Biophys Acta. 1979 Mar 23;552(1):189–193. doi: 10.1016/0005-2736(79)90258-x. [DOI] [PubMed] [Google Scholar]
  31. Yellin N., Levin I. W. Hydrocarbon chain disorder in lipid bilayers. Temperature dependent Raman spectra of 1,2-diacyl phosphatidylcholine-water gels. Biochim Biophys Acta. 1977 Nov 24;489(2):177–190. doi: 10.1016/0005-2760(77)90137-0. [DOI] [PubMed] [Google Scholar]

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