Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1986 Apr;49(4):809–815. doi: 10.1016/S0006-3495(86)83709-2

Scanning fluorescence correlation spectroscopy. I. Theory and simulation of aggregation measurements.

N O Petersen
PMCID: PMC1329532  PMID: 3719067

Abstract

Scanning Fluorescence Correlation Spectroscopy (S-FCS) is introduced as an adaptation of Fluorescence Correlation Spectroscopy (FCS) to measure aggregation in systems, such as biological cell membranes, where diffusion or flow is slow. The theoretical framework for interpretation of S-FCS measurements are discussed in this paper with emphasis on the limitations arising from the sample size and shape. Computer simulations of the experiment demonstrate the potential of the technique and illustrate how some of the limitations may be overcome.

Full text

PDF
809

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cuatrecasas P. Developing concepts in receptor research. Drug Intell Clin Pharm. 1983 May;17(5):357–366. doi: 10.1177/106002808301700507. [DOI] [PubMed] [Google Scholar]
  2. Elson E. L., Reidler J. A. Analysis of cell surface interactions by measurements of lateral mobility. J Supramol Struct. 1979;12(4):481–489. doi: 10.1002/jss.400120408. [DOI] [PubMed] [Google Scholar]
  3. Elson E. L., Webb W. W. Concentration correlation spectroscopy: a new biophysical probe based on occupation number fluctuations. Annu Rev Biophys Bioeng. 1975;4(00):311–334. doi: 10.1146/annurev.bb.04.060175.001523. [DOI] [PubMed] [Google Scholar]
  4. Koppel D. E., Axelrod D., Schlessinger J., Elson E. L., Webb W. W. Dynamics of fluorescence marker concentration as a probe of mobility. Biophys J. 1976 Nov;16(11):1315–1329. doi: 10.1016/S0006-3495(76)85776-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Magde D., Elson E. L., Webb W. W. Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers. 1974 Jan;13(1):29–61. doi: 10.1002/bip.1974.360130103. [DOI] [PubMed] [Google Scholar]
  6. Peters R. Translational diffusion in the plasma membrane of single cells as studied by fluorescence microphotolysis. Cell Biol Int Rep. 1981 Aug;5(8):733–760. doi: 10.1016/0309-1651(81)90231-9. [DOI] [PubMed] [Google Scholar]
  7. Petersen N. O. Diffusion and aggregation in biological membranes. Can J Biochem Cell Biol. 1984 Nov;62(11):1158–1166. doi: 10.1139/o84-149. [DOI] [PubMed] [Google Scholar]
  8. Petersen N. O., Johnson D. C., Schlesinger M. J. Scanning fluorescence correlation spectroscopy. II. Application to virus glycoprotein aggregation. Biophys J. 1986 Apr;49(4):817–820. doi: 10.1016/S0006-3495(86)83710-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Petersen N. O., McConnaughey W. B. Effects of multiple membranes on measurements of cell surface dynamics by fluorescence photobleaching. J Supramol Struct Cell Biochem. 1981;17(3):213–221. doi: 10.1002/jsscb.380170303. [DOI] [PubMed] [Google Scholar]
  10. Roos D. S., Robinson J. M., Davidson R. L. Cell fusion and intramembrane particle distribution in polyethylene glycol-resistant cells. J Cell Biol. 1983 Sep;97(3):909–917. doi: 10.1083/jcb.97.3.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schreiber A. B., Hoebeke J., Vray B., Strosberg A. D. Evidence for reversible microclustering of lentil lectin membrane receptors on HeLa cells. FEBS Lett. 1980 Mar 10;111(2):303–306. doi: 10.1016/0014-5793(80)80815-5. [DOI] [PubMed] [Google Scholar]
  12. Sheetz M. P., Schindler M., Koppel D. E. Lateral mobility of integral membrane proteins is increased in spherocytic erythrocytes. Nature. 1980 Jun 12;285(5765):510–511. doi: 10.1038/285510a0. [DOI] [PubMed] [Google Scholar]
  13. Shotton D., Thompson K., Wofsy L., Branton D. Appearance and distribution of surface proteins of the human erythrocyte membrane. An electron microscope and immunochemical labeling study. J Cell Biol. 1978 Feb;76(2):512–531. doi: 10.1083/jcb.76.2.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Webb W. W., Barak L. S., Tank D. W., Wu E. S. Molecular mobility on the cell surface. Biochem Soc Symp. 1981;(46):191–205. [PubMed] [Google Scholar]
  15. Weissman M., Schindler H., Feher G. Determination of molecular weights by fluctuation spectroscopy: application to DNA. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2776–2780. doi: 10.1073/pnas.73.8.2776. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES