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. 1987 Feb;51(2):339–343. doi: 10.1016/S0006-3495(87)83340-4

Theory of sample translation in fluorescence correlation spectroscopy.

A G Palmer 3rd, N L Thompson
PMCID: PMC1329895  PMID: 3828464

Abstract

New applications of the technique of fluorescence correlation spectroscopy (FCS) require lateral translation of the sample through a focused laser beam (Peterson, N.O., D.C. Johnson, and M.J. Schlesinger, 1986, Biophys. J., 49:817-820). Here, the effect of sample translation on the shape of the FCS autocorrelation function is examined in general. It is found that if the lateral diffusion coefficients of the fluorescent species obey certain conditions, then the FCS autocorrelation function is a simple product of one function that depends only on transport coefficients and another function that depends only on the rate constants of chemical reactions that occur in the sample. This simple form should allow manageable data analyses in new FCS experiments that involve sample translation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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