Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1987 Jun;51(6):849–863. doi: 10.1016/S0006-3495(87)83413-6

A general procedure for determining the rate of calcium release from the sarcoplasmic reticulum in skeletal muscle fibers.

W Melzer, E Rios, M F Schneider
PMCID: PMC1330019  PMID: 3496921

Abstract

A general procedure for using myoplasmic calcium transients measured with a metallochromic indicator dye to calculate the time course of calcium release from the sarcoplasmic reticulum in voltage-clamped skeletal muscle fibers is described and analyzed. Explicit properties are first assigned to all relatively rapidly equilibrating calcium binding sites in the myoplasm so that the calcium content (CaF) in this pool of "fast" calcium can be calculated from the calcium transient. The overall properties of the transport systems and relatively slowly equilibrating binding sites that remove calcium from CaF are then characterized experimentally from the decay of CaF following fiber repolarization. The rate of calcium release can then be calculated as dCaF/dt plus the rate of removal of calcium from CaF. Two alternatives are assumed for the component of CaF that is due to fast binding sites intrinsic to the fiber: a linear instantaneous buffer or a set of binding sites having properties similar to thin filament troponin. Both assumptions yielded similar calcium release wave forms. Three alternative methods for characterizing the removal system are presented. The choice among these or other methods for characterizing removal can be based entirely on convenience since any method that reproduces the decay of CaF following fiber repolarization will give the same release wave form. The calculated release wave form will be accurate provided that the properties assumed for CaF are correct, that release turns off within a relatively short time after fiber repolarization, that the properties of the slow removal system are the same during and after fiber depolarization, and that possible spatial nonuniformities of free or bound calcium do not introduce major errors.

Full text

PDF
849

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashley C. C., Moisescu D. G. Model for the action of calcium in muscle. Nat New Biol. 1972 Jun 14;237(76):208–211. doi: 10.1038/newbio237208a0. [DOI] [PubMed] [Google Scholar]
  2. Baylor S. M., Chandler W. K., Marshall M. W. Sarcoplasmic reticulum calcium release in frog skeletal muscle fibres estimated from Arsenazo III calcium transients. J Physiol. 1983 Nov;344:625–666. doi: 10.1113/jphysiol.1983.sp014959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baylor S. M., Hollingworth S., Hui C. S., Quinta-Ferreira M. E. Properties of the metallochromic dyes Arsenazo III, Antipyrylazo III and Azo1 in frog skeletal muscle fibres at rest. J Physiol. 1986 Aug;377:89–141. doi: 10.1113/jphysiol.1986.sp016178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blinks J. R., Wier W. G., Hess P., Prendergast F. G. Measurement of Ca2+ concentrations in living cells. Prog Biophys Mol Biol. 1982;40(1-2):1–114. doi: 10.1016/0079-6107(82)90011-6. [DOI] [PubMed] [Google Scholar]
  5. Cannell M. B., Allen D. G. Model of calcium movements during activation in the sarcomere of frog skeletal muscle. Biophys J. 1984 May;45(5):913–925. doi: 10.1016/S0006-3495(84)84238-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cannell M. B. Effect of tetanus duration on the free calcium during the relaxation of frog skeletal muscle fibres. J Physiol. 1986 Jul;376:203–218. doi: 10.1113/jphysiol.1986.sp016149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cota G., Nicola Siri L., Stefani E. Calcium-channel gating in frog skeletal muscle membrane: effect of temperature. J Physiol. 1983 May;338:395–412. doi: 10.1113/jphysiol.1983.sp014679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gillis J. M., Thomason D., Lefèvre J., Kretsinger R. H. Parvalbumins and muscle relaxation: a computer simulation study. J Muscle Res Cell Motil. 1982 Dec;3(4):377–398. doi: 10.1007/BF00712090. [DOI] [PubMed] [Google Scholar]
  9. Kovacs L., Rios E., Schneider M. F. Measurement and modification of free calcium transients in frog skeletal muscle fibres by a metallochromic indicator dye. J Physiol. 1983 Oct;343:161–196. doi: 10.1113/jphysiol.1983.sp014887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Melzer W., Rios E., Schneider M. F. Time course of calcium release and removal in skeletal muscle fibers. Biophys J. 1984 Mar;45(3):637–641. doi: 10.1016/S0006-3495(84)84203-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Melzer W., Ríos E., Schneider M. F. The removal of myoplasmic free calcium following calcium release in frog skeletal muscle. J Physiol. 1986 Mar;372:261–292. doi: 10.1113/jphysiol.1986.sp016008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Melzer W., Schneider M. F., Simon B. J., Szucs G. Intramembrane charge movement and calcium release in frog skeletal muscle. J Physiol. 1986 Apr;373:481–511. doi: 10.1113/jphysiol.1986.sp016059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miledi R., Parker I., Zhu P. H. Calcium transients evoked by action potentials in frog twitch muscle fibres. J Physiol. 1982 Dec;333:655–679. doi: 10.1113/jphysiol.1982.sp014474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miledi R., Parker I., Zhu P. H. Calcium transients studied under voltage-clamp control in frog twitch muscle fibres. J Physiol. 1983 Jul;340:649–680. doi: 10.1113/jphysiol.1983.sp014785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rakowski R. F., Best P. M., James-Kracke M. R. Voltage dependence of membrane charge movement and calcium release in frog skeletal muscle fibres. J Muscle Res Cell Motil. 1985 Aug;6(4):403–433. doi: 10.1007/BF00712580. [DOI] [PubMed] [Google Scholar]
  16. Ridgway E. B., Gordon A. M., Martyn D. A. Hysteresis in the force-calcium relation in muscle. Science. 1983 Mar 4;219(4588):1075–1077. doi: 10.1126/science.6823567. [DOI] [PubMed] [Google Scholar]
  17. Ridgway E. B., Gordon A. M. Muscle calcium transient. Effect of post-stimulus length changes in single fibers. J Gen Physiol. 1984 Jan;83(1):75–103. doi: 10.1085/jgp.83.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Robertson S. P., Johnson J. D., Potter J. D. The time-course of Ca2+ exchange with calmodulin, troponin, parvalbumin, and myosin in response to transient increases in Ca2+. Biophys J. 1981 Jun;34(3):559–569. doi: 10.1016/S0006-3495(81)84868-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ríos E., Schneider M. F. Stoichiometry of the reactions of calcium with the metallochromic indicator dyes antipyrylazo III and arsenazo III. Biophys J. 1981 Dec;36(3):607–621. doi: 10.1016/S0006-3495(81)84755-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanchez J. A., Stefani E. Inward calcium current in twitch muscle fibres of the frog. J Physiol. 1978 Oct;283:197–209. doi: 10.1113/jphysiol.1978.sp012496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schneider M. F., Rios E., Melzer W. Use of a metallochromic indicator to study intracellular calcium movements in skeletal muscle. Cell Calcium. 1985 Apr;6(1-2):109–118. doi: 10.1016/0143-4160(85)90038-7. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES