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. 1988 Feb;53(2):145–152. doi: 10.1016/S0006-3495(88)83076-5

23Na-nuclear magnetic resonance investigation of gramicidin-induced ion transport through membranes under equilibrium conditions.

D C Buster 1, J F Hinton 1, F S Millett 1, D C Shungu 1
PMCID: PMC1330135  PMID: 2449917

Abstract

A technique for investigating the gramicidin-facilitated transport of Na+ ions across lipid bilayers of large unilamellar vesicles under the condition of ionic equilibrium has been developed using a combination of heat incubation of the gramicidin with the vesicles and 23Na-nuclear magnetic resonance (NMR) spectroscopy. Isolation of the two 23Na-NMR signals from the intra- and extravesicular Na+ with the shift reagent, dysprosium (III) tripolyphosphate, allows the equilibrium flux of Na+ through the gramicidin channels to be detected and treated as a two-site exchange process. This study indicates that the transport of Na+ through gramicidin channels is second order with respect to the gramicidin concentration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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