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. 1988 Feb;53(2):287–292. doi: 10.1016/S0006-3495(88)83091-1

Patch electrode glass composition affects ion channel currents.

R E Furman 1, J C Tanaka 1
PMCID: PMC1330150  PMID: 2449920

Abstract

The influence of patch electrode glass composition on macroscopic IV relations in inside-out patches of the cGMP-activated ion channel from rod photoreceptors was examined for a soda lime glass, a Kovar sealing glass, a borosilicate glass, and several soft lead glasses. In several glasses the shape or magnitude of the currents changed as the concentration of EGTA or EDTA was increased from 200 microM to 10 mM. The changes in IV response suggest that, at low concentrations of chelator, divalent cations are released from the electrode glass and interact with the cGMP-activated channel. Possible mechanisms are discussed to explain the observations, and several comments are made concerning the choice of a glass for patching.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baylor D. A., Nunn B. J. Electrical properties of the light-sensitive conductance of rods of the salamander Ambystoma tigrinum. J Physiol. 1986 Feb;371:115–145. doi: 10.1113/jphysiol.1986.sp015964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EISENMAN G. Cation selective glass electrodes and their mode of operation. Biophys J. 1962 Mar;2(2 Pt 2):259–323. doi: 10.1016/s0006-3495(62)86959-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  4. Haynes L. W., Kay A. R., Yau K. W. Single cyclic GMP-activated channel activity in excised patches of rod outer segment membrane. Nature. 1986 May 1;321(6065):66–70. doi: 10.1038/321066a0. [DOI] [PubMed] [Google Scholar]
  5. Hodgkin A. L., McNaughton P. A., Nunn B. J. The ionic selectivity and calcium dependence of the light-sensitive pathway in toad rods. J Physiol. 1985 Jan;358:447–468. doi: 10.1113/jphysiol.1985.sp015561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Neher E., Sakmann B. Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature. 1976 Apr 29;260(5554):799–802. doi: 10.1038/260799a0. [DOI] [PubMed] [Google Scholar]
  7. Tanaka J. C., Furman R. E., Cobbs W. H., Mueller P. Incorporation of a retinal rod cGMP-dependent conductance into planar bilayers. Proc Natl Acad Sci U S A. 1987 Feb;84(3):724–728. doi: 10.1073/pnas.84.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yau K. W., Nakatani K. Cation selectivity of light-sensitive conductance in retinal rods. Nature. 1984 May 24;309(5966):352–354. doi: 10.1038/309352a0. [DOI] [PubMed] [Google Scholar]
  9. Zimmerman A. L., Baylor D. A. Cyclic GMP-sensitive conductance of retinal rods consists of aqueous pores. Nature. 1986 May 1;321(6065):70–72. doi: 10.1038/321070a0. [DOI] [PubMed] [Google Scholar]

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