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. 1988 Sep;54(3):489–494. doi: 10.1016/S0006-3495(88)82981-3

Anion conductances of the giant axon of squid Sepioteuthis.

I Inoue 1
PMCID: PMC1330347  PMID: 3207836

Abstract

Anion conductances of giant axons of squid, Sepioteuthis, were measured. The axons were internally perfused with a 100-mM tetraethylammonium-phosphate solution and immersed in a 100-mM Ca-salt solution (or Mg-salt solution) containing 0.3 microns tetrodotoxin. The external anion composition was changed. The membrane currents had a large amount of outward rectification due to anion influx across Cl- channels of the membrane (Inoue, 1985). The amount of outward rectification depended on the species of anion used and was strongly influenced by temperature and internal pH. In contrast to the anion conductances themselves, the conductance relative to Cl- (gA/gCl) was found to be quite stable against changes in the membrane potential, temperature, and pH. It is therefore suggested that each gA/gCl is an intrinsic quantity of the Cl- channel of the squid axon membrane. The sequence and values of gA/gCl obtained in this study were NO3- (1.80) greater than I- (1.40) greater than Br- (1.07) greater than Cl- (1.00) greater than MeSO3- (0.46) greater than H2PO2- (0.33) greater than CH3COO- (0.29) greater than SO4(2-) (0.06).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADRIAN R. H. Internal chloride concentration and chloride efflux of frog muscle. J Physiol. 1961 May;156:623–632. doi: 10.1113/jphysiol.1961.sp006698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BAKER P. F., HODGKIN A. L., SHAW T. I. The effects of changes in internal ionic concentrations on the electrical properties of perfused giant axons. J Physiol. 1962 Nov;164:355–374. doi: 10.1113/jphysiol.1962.sp007026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bormann J., Hamill O. P., Sakmann B. Mechanism of anion permeation through channels gated by glycine and gamma-aminobutyric acid in mouse cultured spinal neurones. J Physiol. 1987 Apr;385:243–286. doi: 10.1113/jphysiol.1987.sp016493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cabantchik Z. I., Rothstein A. The nature of the membrane sites controlling anion permeability of human red blood cells as determined by studies with disulfonic stilbene derivatives. J Membr Biol. 1972 Dec 29;10(3):311–330. doi: 10.1007/BF01867863. [DOI] [PubMed] [Google Scholar]
  5. Conti F., Inoue I., Kukita F., Stühmer W. Pressure dependence of sodium gating currents in the squid giant axon. Eur Biophys J. 1984;11(2):137–147. doi: 10.1007/BF00276629. [DOI] [PubMed] [Google Scholar]
  6. DiPolo R. Chloride fluxes in isolated dialyzed barnacle muscle fibers. J Gen Physiol. 1972 Oct;60(4):471–497. doi: 10.1085/jgp.60.4.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  8. HARRIS E. J. Anion interaction in frog muscle. J Physiol. 1958 Apr 30;141(2):351–365. doi: 10.1113/jphysiol.1958.sp005979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HARRIS E. J. THE CHLORIDE PERMEABILITY OF FROG SARTORIUS. J Physiol. 1965 Jan;176:123–135. doi: 10.1113/jphysiol.1965.sp007539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HODGKIN A. L., HOROWICZ P. The influence of potassium and chloride ions on the membrane potential of single muscle fibres. J Physiol. 1959 Oct;148:127–160. doi: 10.1113/jphysiol.1959.sp006278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HODGKIN A. L., HUXLEY A. F., KATZ B. Measurement of current-voltage relations in the membrane of the giant axon of Loligo. J Physiol. 1952 Apr;116(4):424–448. doi: 10.1113/jphysiol.1952.sp004716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HUTTER O. F., PADSHA S. M. Effect of nitrate and other anions on the membrane resistance of frog skeletal muscle. J Physiol. 1959 Apr 23;146(1):117–132. doi: 10.1113/jphysiol.1959.sp006182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hagiwara S., Takahashi K. Mechanism of anion permeation through the muscle fibre membrane of an elasmobranch fish, Taeniura lymma. J Physiol. 1974 Apr;238(1):109–127. doi: 10.1113/jphysiol.1974.sp010513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hille B., Schwarz W. Potassium channels as multi-ion single-file pores. J Gen Physiol. 1978 Oct;72(4):409–442. doi: 10.1085/jgp.72.4.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ho M. K., Guidotti G. A membrane protein from human erythrocytes involved in anion exchange. J Biol Chem. 1975 Jan 25;250(2):675–683. [PubMed] [Google Scholar]
  16. Hutter O. F., Warner A. E. Action of some foreign cations and anions on the chloride permeability of frog muscle. J Physiol. 1967 Apr;189(3):445–460. doi: 10.1113/jphysiol.1967.sp008178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hutter O. F., Warner A. E. The pH sensitivity of the chloride conductance of frog skeletal muscle. J Physiol. 1967 Apr;189(3):403–425. doi: 10.1113/jphysiol.1967.sp008176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Inoue I. Modification of K conductance of the squid axon membrane by SITS. J Gen Physiol. 1986 Oct;88(4):507–520. doi: 10.1085/jgp.88.4.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Inoue I., Pant H. C., Tasaki I., Gainer H. Release of proteins from the inner surface of squid axon membrane labeled with tritiated N-ethylmaleimide. J Gen Physiol. 1976 Oct;68(4):385–395. doi: 10.1085/jgp.68.4.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Inoue I. Voltage-dependent chloride conductance of the squid axon membrane and its blockade by some disulfonic stilbene derivatives. J Gen Physiol. 1985 Apr;85(4):519–537. doi: 10.1085/jgp.85.4.519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kasai M., Kometani T. Inhibition of anion permeability of sarcoplasmic reticulum vesicles by 4-acetoamido-4'-isothiocyanostilbene-2,2'-disulfonate. Biochim Biophys Acta. 1979 Oct 19;557(1):243–247. doi: 10.1016/0005-2736(79)90106-8. [DOI] [PubMed] [Google Scholar]
  22. Knauf P. A., Rothstein A. Chemical modification of membranes. I. Effects of sulfhydryl and amino reactive reagents on anion and cation permeability of the human red blood cell. J Gen Physiol. 1971 Aug;58(2):190–210. doi: 10.1085/jgp.58.2.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moore L. E. Anion permeability of frog skeletal muscle. J Gen Physiol. 1969 Jul;54(1):33–52. doi: 10.1085/jgp.54.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Palade P. T., Barchi R. L. Characteristics of the chloride conductance in muscle fibers of the rat diaphragm. J Gen Physiol. 1977 Mar;69(3):325–342. doi: 10.1085/jgp.69.3.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rothstein A., Cabantchik Z. I., Knauf P. Mechanism of anion transport in red blood cells: role of membrane proteins. Fed Proc. 1976 Jan;35(1):3–10. [PubMed] [Google Scholar]
  26. Takeuchi A., Takeuchi N. Anion interaction at the inhibitory post-synaptic membrane of the crayfish neuromuscular junction. J Physiol. 1971 Jan;212(2):337–351. doi: 10.1113/jphysiol.1971.sp009328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tasaki I., Singer I., Takenaka T. Effects of internal and external ionic environment on excitability of squid giant axon. A macromolecular approach. J Gen Physiol. 1965 Jul;48(6):1095–1123. doi: 10.1085/jgp.48.6.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. White M. M., Miller C. Probes of the conduction process of a voltage-gated Cl- channel from Torpedo electroplax. J Gen Physiol. 1981 Jul;78(1):1–18. doi: 10.1085/jgp.78.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]

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