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. 1988 Sep;54(3):545–549. doi: 10.1016/S0006-3495(88)82987-4

Picosecond transient absorption study of photodissociated carboxy hemoglobin and myoglobin.

S M Janes 1, G A Dalickas 1, W A Eaton 1, R M Hochstrasser 1
PMCID: PMC1330353  PMID: 3207839

Abstract

The optical transient absorption spectra at 30 ps and 6.5 ns after photolysis are compared for both carboxy hemoglobin (HbCO) and carboxy myoglobin (MbCO). Both 355- and 532-nm excitation pulses were used. In all cases the shapes of the optical difference spectra thus generated are stationary over the complete time-scale studied. The photolysis spectra for MbCO are not significantly different from the equilibrium difference spectra generated on the same picosecond spectrometer when measured to an accuracy of +/- 0.5 nm. In addition, spectral parameters for delegated HbCO generated on the same spectrometer but detected by two different techniques, either by a Vidicon detector or point by point with photomultiplier tubes, are reported; the results are different from some of the previously reported picosecond experiments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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