Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1989 Mar;55(3):519–526. doi: 10.1016/S0006-3495(89)82845-0

System analysis of Phycomyces light-growth response: madC, madG, and madH mutants.

A Palit 1, P R Pratap 1, E D Lipson 1
PMCID: PMC1330505  PMID: 2930832

Abstract

The light-growth response of Phycomyces has been studied further with the sum-of-sinusoids method in the framework of the Wiener theory of nonlinear system identification. The response was treated as a black box with the logarithm of light intensity as the input and elongation rate as the output. The nonlinear input-output relation of the light-growth response can be represented mathematically by a set of weighting functions called kernels, which appear in the Wiener intergral series. The linear (first-order) kernels of wild type, and of single and double mutants affected in genes madA to madG were determined previously with Gaussian white noise test stimuli, and were used to investigate the interactions among the products of these genes (R.C. Poe, P. Pratap, and E.D. Lipson. 1986. Biol. Cybern. 55:105.). We have used the more precise sum-of-sinusoids method to extend the interaction studies, including both the first- and second-order kernels. Specifically, we have investigated interactions of the madH ("hypertropic") gene product with the madC ("night blind") and madG ("stiff") gene products. Experiments were performed on the Phycomyces tracking machine. The log-mean intensity of the stimulus was 6 x 10(-2) W m-2 and the wavelength was 477 nm. The first- and second-order kernels were analyzed in terms of nonlinear kinetic models. The madH gene product was found to interact with those of madC and madG. This result extends previous findings that themadH gene product is associated with the input and the ouput of the sensory transduction complex for the lightgrowth response.

Full text

PDF
519

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Foster K. W., Lipson E. D. The light growth response of Phycomyces. J Gen Physiol. 1973 Nov;62(5):590–617. doi: 10.1085/jgp.62.5.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Galland P., Lipson E. D. Modified action spectra of photogeotropic equilibrium in Phycomyces blakesleeanus mutants with defects in genes madA, madB, madC, and madH. Photochem Photobiol. 1985 Mar;41(3):331–335. doi: 10.1111/j.1751-1097.1985.tb03493.x. [DOI] [PubMed] [Google Scholar]
  3. Lipson E. D. White noise analysis of Phycomyces light growth response system. I. Normal intensity range. Biophys J. 1975 Oct;15(10):989–1011. doi: 10.1016/S0006-3495(75)85879-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lipson E. D. White noise analysis of Phycomyces light growth response system. II. Extended intensity ranges. Biophys J. 1975 Oct;15(10):1013–1031. doi: 10.1016/S0006-3495(75)85880-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lipson E. D. White noise analysis of Phycomyces light growth response system. III. Photomutants. Biophys J. 1975 Oct;15(10):1033–1045. doi: 10.1016/S0006-3495(75)85881-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. López-Díaz I., Lipson E. D. Genetic analysis of hypertropic mutants of Phycomyces. Mol Gen Genet. 1983;190(2):318–325. doi: 10.1007/BF00330658. [DOI] [PubMed] [Google Scholar]
  7. Palit A., Pratap P., Lipson E. D. System analysis of Phycomyces light-growth response. Photoreceptor and hypertropic mutants. Biophys J. 1986 Oct;50(4):661–668. doi: 10.1016/S0006-3495(86)83506-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Poe R. C., Lipson E. D. System analysis of Phycomyces light-growth response with Gaussian white-noise test stimuli. Biol Cybern. 1986;55(2-3):91–98. doi: 10.1007/BF00341924. [DOI] [PubMed] [Google Scholar]
  9. Poe R. C., Pratap P., Lipson E. D. System analysis of Phycomyces light-growth response: double mutants. Biol Cybern. 1986;55(2-3):105–113. doi: 10.1007/BF00341926. [DOI] [PubMed] [Google Scholar]
  10. Poe R. C., Pratap P., Lipson E. D. System analysis of Phycomyces light-growth response: single mutants. Biol Cybern. 1986;55(2-3):99–104. doi: 10.1007/BF00341925. [DOI] [PubMed] [Google Scholar]
  11. Pratap P., Palit A., Lipson E. D. System analysis of Phycomyces light-growth response with sum-of-sinusoids test stimuli. Biophys J. 1986 Oct;50(4):645–651. doi: 10.1016/S0006-3495(86)83504-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pratap P., Palit A., Lipson E. D. System analysis of Phycomyces light-growth response. Wavelength and temperature dependence. Biophys J. 1986 Oct;50(4):653–660. doi: 10.1016/S0006-3495(86)83505-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Victor J., Shapley R. A method of nonlinear analysis in the frequency domain. Biophys J. 1980 Mar;29(3):459–483. doi: 10.1016/S0006-3495(80)85146-0. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES