Abstract
Deconvolution of ultrasonic data into single relaxations is rarely feasible when only the absorption or the velocity of the waves is measured. Here we use both series of data to construct a Cole-Cole diagram for a solution. When applied to alpha-chymotrypsin, this method shows two relaxations that are well separated on the time scale, a result that will help simplify analyses of the ultrasonic data for this enzyme.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bieth J., Métais P., Warter J. Etude des protéases pancréatiques. II. --Dosage de la chymotrypsine par la succinyl-phénylalanine-p-nitroanilide et ses applications. Ann Biol Clin (Paris) 1968 Jan-Feb;26(1):143–158. [PubMed] [Google Scholar]
- Cerf R. Absolute measurement of enhanced fluctuations in assemblies of biomolecules by ultrasonic techniques. Biophys J. 1985 Jun;47(6):751–756. doi: 10.1016/S0006-3495(85)83977-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cerf R., Dormoy Y. Ultrasonic absorption evidence for enhanced volume fluctuations in the tobacco mosaic virus protein helical aggregate. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8147–8151. doi: 10.1073/pnas.83.21.8147. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cerf R., Michels B., Schulz J. A., Witz J., Pfeiffer P., Hirth L. Ultrasonic absorption evidence of structural fluctuations in viral capsids. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1780–1782. doi: 10.1073/pnas.76.4.1780. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michels B., Dormoy Y., Cerf R., Schulz J. A., Witz J. Ultrasonic absorption in tobacco mosaic virus and its protein aggregates. J Mol Biol. 1985 Jan 5;181(1):103–110. doi: 10.1016/0022-2836(85)90328-6. [DOI] [PubMed] [Google Scholar]
- Robach Y., Michels B., Cerf R., Braunwald J., Tripier-Darcy F. Ultrasonic absorption evidence for structural fluctuations in frog virus 3 and its subparticles. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3981–3985. doi: 10.1073/pnas.80.13.3981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rogez D., Cerf R., Andrianjara R., Salehi S. T., Fouladgar H. Ultrasonic studies of proton-transfer reactions at the catalytic site of alpha-chymotrypsin. FEBS Lett. 1987 Jul 13;219(1):22–26. doi: 10.1016/0014-5793(87)81183-3. [DOI] [PubMed] [Google Scholar]