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. 1974 Sep;241(3):761–770. doi: 10.1113/jphysiol.1974.sp010683

The absorption of 125I-labelled immunoglobulin G by different regions of the gut in young rats

B Morris, R Morris
PMCID: PMC1331062  PMID: 4436816

Abstract

1. 125I-labelled homologous IgG was injected into different regions of the small intestine of rats aged 12, 16, 18, 20 and 22 days. At 12 days the proximal and middle regions of the intestine readily absorbed globulin and transmitted it to the circulation. The distal region of the intestine transmitted little to the circulation at all ages tested.

2. The intestine loses its ability to transmit globulin to the circulation in a distal-proximal direction. At 16 and 18 days the ability of the middle region had declined significantly, and this decline continued so that little globulin was transmitted from this region at 20 and 22 days.

3. The proximal intestine retained the ability to transmit globulin to the circulation in significant amounts up to 20 days.

4. There is a close negative correlation between body weight and total radioactivity of the sera of rats which had received doses of labelled globulin into the proximal and middle regions of the intestine. There was no such correlation after injection into the distal intestine — suggesting a restricted throughput of radioactive material by the absorptive cells of this region.

5. These results are discussed in the context of the termination of antibody absorption, and in relation to the results obtained using polyvinyl pyrrolidone.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CLARK S. L., Jr The ingestion of proteins and colloidal materials by columnar absorptive cells of the small intestine in suckling rats and mice. J Biophys Biochem Cytol. 1959 Jan 25;5(1):41–50. doi: 10.1083/jcb.5.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clarke R. M., Hardy R. N. An analysis of the mechanism of cessation of uptake of macromolecular substances by the intestine of the young rat ('closure'). J Physiol. 1969 Sep;204(1):127–134. doi: 10.1113/jphysiol.1969.sp008903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clarke R. M., Hardy R. N. The use of [125-I] polyvinyl pyrrolidone K. 60 in the quantitative assessment of the uptake of macromolecular substances by the intestine of the young rat. J Physiol. 1969 Sep;204(1):113–125. doi: 10.1113/jphysiol.1969.sp008902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daniels V. G., Hardy R. N., Malinowska K. W., Nathanielsz P. W. The influence of exogenous steroids on macromolecule uptake by the small intestine of the new-born rat. J Physiol. 1973 Mar;229(3):681–695. doi: 10.1113/jphysiol.1973.sp010160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Daniels V. G., Hardy R. N., Malinowska K. W. The effect of adrenalectomy or pharmacological inhibition of adrenocortical function on macromolecule uptake by the new-born rat intestine. J Physiol. 1973 Mar;229(3):697–707. doi: 10.1113/jphysiol.1973.sp010161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graney D. O. The uptake of ferritin by ileal absorptive cells in suckling rats. An electron microscope study. Am J Anat. 1968 Sep;123(2):227–254. doi: 10.1002/aja.1001230202. [DOI] [PubMed] [Google Scholar]
  7. HALLIDAY R. The absorption of antibodies from immune sera by the gut of the young rat. Proc R Soc Lond B Biol Sci. 1955 Mar 15;143(912):408–413. doi: 10.1098/rspb.1955.0020. [DOI] [PubMed] [Google Scholar]
  8. Jones R. E. Intestinal absorption and degradation of rat and bovine gamma-globulins in the suckling rat. Biochim Biophys Acta. 1972 Feb 11;255(2):530–538. doi: 10.1016/0005-2736(72)90156-3. [DOI] [PubMed] [Google Scholar]
  9. Morris B., Morris R. The effects of cortisone acetate on stomach evacuation and the absorption of 125I-labelled globulins in young rats. J Physiol. 1974 Jul;240(1):79–89. doi: 10.1113/jphysiol.1974.sp010600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morris I. G. The transmission of anti-Brucella abortus agglutinins across the gut in young rats. Proc R Soc Lond B Biol Sci. 1965 Nov 23;163(992):402–416. doi: 10.1098/rspb.1965.0075. [DOI] [PubMed] [Google Scholar]
  11. Rodewald R. Intestinal transport of antibodies in the newborn rat. J Cell Biol. 1973 Jul;58(1):189–211. doi: 10.1083/jcb.58.1.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosa U., Pennisi F., Bianchi R., Federighi G., Donato L. Chemical and biological effects of iodination on human albumin. Biochim Biophys Acta. 1967 Apr 11;133(3):486–498. doi: 10.1016/0005-2795(67)90553-3. [DOI] [PubMed] [Google Scholar]
  13. Webb A. J. A 30-minute preparative method for isolation of IgG from human serum. Vox Sang. 1972;23(4):279–290. doi: 10.1111/j.1423-0410.1972.tb03462.x. [DOI] [PubMed] [Google Scholar]

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