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. 1972 Nov;226(3):761–768. doi: 10.1113/jphysiol.1972.sp010008

The effects of calcium and magnesium on statistical release parameters at the crayfish neuromuscular junction

A Wernig
PMCID: PMC1331175  PMID: 4404687

Abstract

1. Transmitter release at excitatory neuromuscular junctions of crayfish muscle was studied at low temperature by recording synaptic potentials with extracellular micro-electrodes.

2. Increasing the Ca concentration in the bathing solution produced an increase in the average number of quanta released per nerve stimulus (m). Increasing the Mg concentration resulted in a decrease in m.

3. Statistical analysis of fluctuations in the quantal release from trial to trial, assuming binomial statistics, indicated that both the changes in m were due to changes in the average quantal release probability (p).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker P. F., Hodgkin A. L., Ridgway E. B. Depolarization and calcium entry in squid giant axons. J Physiol. 1971 Nov;218(3):709–755. doi: 10.1113/jphysiol.1971.sp009641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Betz W. J. Depression of transmitter release at the neuromuscular junction of the frog. J Physiol. 1970 Mar;206(3):629–644. doi: 10.1113/jphysiol.1970.sp009034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bittner G. D. Differentiation of nerve terminals in the crayfish opener muscle and its functional significance. J Gen Physiol. 1968 Jun;51(6):731–758. doi: 10.1085/jgp.51.6.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bracho H., Orkand R. K. Effect of calcium on excitatory neuromuscular transmission in the crayfish. J Physiol. 1970 Jan;206(1):61–71. doi: 10.1113/jphysiol.1970.sp008997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christensen B. N., Martin A. R. Estimates of probability of transmitter release at the mammalian neuromuscular junction. J Physiol. 1970 Nov;210(4):933–945. doi: 10.1113/jphysiol.1970.sp009250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DEL CASTILLO J., KATZ B. Quantal components of the end-plate potential. J Physiol. 1954 Jun 28;124(3):560–573. doi: 10.1113/jphysiol.1954.sp005129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dodge F. A., Jr, Rahamimoff R. Co-operative action a calcium ions in transmitter release at the neuromuscular junction. J Physiol. 1967 Nov;193(2):419–432. doi: 10.1113/jphysiol.1967.sp008367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Elmqvist D., Quastel D. M. A quantitative study of end-plate potentials in isolated human muscle. J Physiol. 1965 Jun;178(3):505–529. doi: 10.1113/jphysiol.1965.sp007639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson E. W., Wernig A. The binomial nature of transmitter release at the crayfish neuromuscular junction. J Physiol. 1971 Nov;218(3):757–767. doi: 10.1113/jphysiol.1971.sp009642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Katz B., Miledi R. A study of synaptic transmission in the absence of nerve impulses. J Physiol. 1967 Sep;192(2):407–436. doi: 10.1113/jphysiol.1967.sp008307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Katz B., Miledi R. Further study of the role of calcium in synaptic transmission. J Physiol. 1970 May;207(3):789–801. doi: 10.1113/jphysiol.1970.sp009095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Katz B., Miledi R. The effect of temperature on the synaptic delay at the neuromuscular junction. J Physiol. 1965 Dec;181(3):656–670. doi: 10.1113/jphysiol.1965.sp007790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Katz B., Miledi R. The release of acetylcholine from nerve endings by graded electric pulses. Proc R Soc Lond B Biol Sci. 1967 Jan 31;167(1006):23–38. doi: 10.1098/rspb.1967.0011. [DOI] [PubMed] [Google Scholar]
  14. Katz B., Miledi R. The role of calcium in neuromuscular facilitation. J Physiol. 1968 Mar;195(2):481–492. doi: 10.1113/jphysiol.1968.sp008469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Katz B., Miledi R. The timing of calcium action during neuromuscular transmission. J Physiol. 1967 Apr;189(3):535–544. doi: 10.1113/jphysiol.1967.sp008183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LILEY A. W., NORTH K. A. An electrical investigation of effects of repetitive stimulation on mammalian neuromuscular junction. J Neurophysiol. 1953 Sep;16(5):509–527. doi: 10.1152/jn.1953.16.5.509. [DOI] [PubMed] [Google Scholar]
  17. LUNDBERG A., QUILISCH H. On the effect of calcium on presynaptic potentiation and depression at the neuro-muscular junction. Acta Physiol Scand Suppl. 1953;111:121–129. [PubMed] [Google Scholar]
  18. LUNDBERG A., QUILISCH H. Presynaptic potentiation and depression of neuromuscular transmission in frog and rat. Acta Physiol Scand Suppl. 1953;111:111–120. [PubMed] [Google Scholar]
  19. Rahamimoff R. A dual effect of calcium ions on neuromuscular facilitation. J Physiol. 1968 Mar;195(2):471–480. doi: 10.1113/jphysiol.1968.sp008468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. TAKEUCHI A. The long-lasting depression in neuromuscular transmission of frog. Jpn J Physiol. 1958 Jun 15;8(2):102–113. doi: 10.2170/jjphysiol.8.102. [DOI] [PubMed] [Google Scholar]
  21. THIES R. E. NEUROMUSCULAR DEPRESSION AND THE APPARENT DEPLETION OF TRANSMITTER IN MAMMALIAN MUSCLE. J Neurophysiol. 1965 May;28:428–442. doi: 10.1152/jn.1965.28.3.427. [DOI] [PubMed] [Google Scholar]
  22. Wernig A. Changes in statistical parameters during facilitation at the crayfish neuromuscular junction. J Physiol. 1972 Nov;226(3):751–759. doi: 10.1113/jphysiol.1972.sp010007. [DOI] [PMC free article] [PubMed] [Google Scholar]

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