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. 1973 Feb;228(3):733–748. doi: 10.1113/jphysiol.1973.sp010109

Effect of sodium and sodium-substitutes on the active ion transport and on the membrane potential of smooth muscle cells

R Casteels, G Droogmans, H Hendrickx
PMCID: PMC1331249  PMID: 4702154

Abstract

1. The changes of the ion content and of the membrane potential of taenia coli cells have been studied during prolonged exposure to Na-deficient solutions containing either Li or choline.

2. A K-free solution containing either 71 mM-Na-71 mM-Li or 71 mM-Na-71 mM choline causes a slower loss of cellular K than a 142 mM-Na solution. In both these Na-deficient solutions the membrane hyperpolarizes to about -100 mV for periods up to 6 hr. This hyperpolarization is partially abolished by 2 × 10-5 M ouabain.

3. Replacing all extracellular Na by Li and maintaining 5.9 mM-K causes a fast loss of all Na and a progressive replacement of K by Li. These changes of the intracellular ion content are accompanied by a depolarization of the cells, suggesting that intracellular Li cannot substitute for Na in activating the ion pump.

4. Exposing K-depleted cells to a K-free 71 mM-Na-71 mM-Li solution results in a ouabain sensitive transport of Na and Li against their electro-chemical gradient.

5. The K-uptake by K-depleted cells from a solution containing 0.59 mM-K is increased by reducing [Na]o to half of its normal value. This finding indicates that external Na inhibits the active Na-K exchange.

6. In Na-enriched tissues half of the Na efflux is due to a ouabain insensitive Na-exchange diffusion. If Li is used as a Na substitute, the Na-Li exchange compensates for the diminution of the Na-exchange diffusion unless ouabain is added.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BULBRING E., KURIYAMA H. Effects of changes in the external sodium and calcium concentrations on spontaneous electrical activity in smooth muscle of guinea-pig taenia coli. J Physiol. 1963 Apr;166:29–58. doi: 10.1113/jphysiol.1963.sp007089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker P. F., Blaustein M. P., Keynes R. D., Manil J., Shaw T. I., Steinhardt R. A. The ouabain-sensitive fluxes of sodium and potassium in squid giant axons. J Physiol. 1969 Feb;200(2):459–496. doi: 10.1113/jphysiol.1969.sp008703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker P. F., Connelly C. M. Some properties of the external activation site of the sodium pump in crab nerve. J Physiol. 1966 Jul;185(2):270–297. doi: 10.1113/jphysiol.1966.sp007987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beaugé L. A., Sjodin R. A. The dual effect of lithium ions on sodium efflux in skeletal muscle. J Gen Physiol. 1968 Sep;52(3):408–423. doi: 10.1085/jgp.52.3.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casteels R. Calculation of the membrane potential in smooth muscle cells of the guinea-pig's taenia coli by the Goldman equation. J Physiol. 1969 Nov;205(1):193–208. doi: 10.1113/jphysiol.1969.sp008960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Casteels R., Droogmans G., Hendrickx H. Electrogenic sodium pump in smooth muscle cells of the guinea-pig's taenia coli. J Physiol. 1971 Sep;217(2):297–313. doi: 10.1113/jphysiol.1971.sp009572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casteels R., Droogmans G., Hendrickx H. Membrane potential of smooth muscle cells in K-free solution. J Physiol. 1971 Sep;217(2):281–295. doi: 10.1113/jphysiol.1971.sp009571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Garrahan P. J., Glynn I. M. Facftors affecting the relative magnitudes of the sodium:potassium and sodium:sodium exchanges catalysed by the sodium pump. J Physiol. 1967 Sep;192(1):189–216. doi: 10.1113/jphysiol.1967.sp008296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gorman A. L., Marmor M. F. Contributions of the sodium pump and ionic gradients to the membrane potential of a molluscan neurone. J Physiol. 1970 Nov;210(4):897–917. doi: 10.1113/jphysiol.1970.sp009248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Horowicz P., Taylor J. W., Waggoner D. M. Fractionation of sodium effux in frog sartorius muscles by strophanthidin and removal of external sodium. J Gen Physiol. 1970 Mar;55(3):401–425. doi: 10.1085/jgp.55.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KEYNES R. D., SWAN R. C. The effect of external sodium concentration on the sodium fluxes in frog skeletal muscle. J Physiol. 1959 Oct;147:591–625. doi: 10.1113/jphysiol.1959.sp006264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maizels M. Effect of sodium content on sodium efflux from human red cells suspended in sodium-free media containing potassium, rubidium, caesium or lithium chloride. J Physiol. 1968 Apr;195(3):657–679. doi: 10.1113/jphysiol.1968.sp008481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McConaghey P. D., Maizels M. Cation exchanges of lactose-treated human red cells. J Physiol. 1962 Aug;162(3):485–509. doi: 10.1113/jphysiol.1962.sp006946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. POST R. L., MERRITT C. R., KINSOLVING C. R., ALBRIGHT C. D. Membrane adenosine triphosphatase as a participant in the active transport of sodium and potassium in the human erythrocyte. J Biol Chem. 1960 Jun;235:1796–1802. [PubMed] [Google Scholar]
  15. Priestland R. N., Whittam R. The influence of external sodium ions on the sodium pump in erythrocytes. Biochem J. 1968 Sep;109(3):369–374. doi: 10.1042/bj1090369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rang H. P., Ritchie J. M. On the electrogenic sodium pump in mammalian non-myelinated nerve fibres and its activation by various external cations. J Physiol. 1968 May;196(1):183–221. doi: 10.1113/jphysiol.1968.sp008502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sjodin R. A. The kinetics of sodium extrusion in striated muscle as functions of the external sodium and potassium ion concentrations. J Gen Physiol. 1971 Feb;57(2):164–187. doi: 10.1085/jgp.57.2.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. USSING H. H. Transport of ions across cellular membranes. Physiol Rev. 1949 Apr;29(2):127–155. doi: 10.1152/physrev.1949.29.2.127. [DOI] [PubMed] [Google Scholar]
  19. Wespi H. H. Active transport and passive fluxes of K, Na, and Li in mammalian non-myelinated nerve fibres. Pflugers Arch. 1969;306(3):262–280. doi: 10.1007/BF00592437. [DOI] [PubMed] [Google Scholar]
  20. Whittam R., Ager M. E. Vectorial aspects of adenosine-triphosphatase activity in erythrocyte membranes. Biochem J. 1964 Nov;93(2):337–348. doi: 10.1042/bj0930337. [DOI] [PMC free article] [PubMed] [Google Scholar]

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