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. 1972 Dec;227(1):87–94.2. doi: 10.1113/jphysiol.1972.sp010021

Demonstration of choline acetyltransferase activity in the carotid body of the cat

Kathleen J Ballard, J V Jones
PMCID: PMC1331264  PMID: 4646587

Abstract

1. The distribution of choline acetyltransferase in the carotid body of the cat has been investigated with the electron microscope to determine sites of enzymic activity. This is of relevance to the possible role of acetylcholine as a transmitter in the carotid body.

2. Tissues were fixed for short periods and incubated by the method of Kasa, Mann & Hebb, for the fine structural localization of choline acetyltransferase.

3. The enzyme was found in the cytoplasm of the type I cells and seemed to be associated with vesicles. No enzyme was found in the large nerve endings synapsing with the type I cell.

4. Whole carotid bodies were assayed for their choline acetyltransferase activity and significant amounts were found.

5. It is concluded that acetylcholine may be a transmitter in the carotid body and that it is synthesized in type I cells. A possible mode of initiation of chemoreceptor afferent impulses is suggested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballard K. J., Jones J. V. The fine structural localization of cholinesterases in the carotid body of the cat. J Physiol. 1971 Dec;219(3):747–753. doi: 10.1113/jphysiol.1971.sp009687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Becker A. E., Drukker J., Meijer A. E. Histochemical characteristics of chemoreceptor organs (glomera). Histochemie. 1967;11(3):195–204. doi: 10.1007/BF00306368. [DOI] [PubMed] [Google Scholar]
  3. Biscoe T. J., Silver A. The distribution of cholinesterases in the cat carotid body. J Physiol. 1966 Mar;183(2):501–512. doi: 10.1113/jphysiol.1966.sp007880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Biscoe T. J., Stehbens W. E. Ultrastructure of the carotid body. J Cell Biol. 1966 Sep;30(3):563–578. doi: 10.1083/jcb.30.3.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burt A. M. A histochemical procedure for the localization of choline acetyltransferase activity. J Histochem Cytochem. 1970 Jun;18(6):408–415. doi: 10.1177/18.6.408. [DOI] [PubMed] [Google Scholar]
  6. Duncan D., Yates R. Ultrastructure of the carotid body of the cat as revealed by various fixatives and the use of reserpine. Anat Rec. 1967 Apr;157(4):667–681. doi: 10.1002/ar.1091570412. [DOI] [PubMed] [Google Scholar]
  7. Eyzaguirre C., Koyano H., Taylor J. R. Presence of acetylcholine and transmitter release from carotid body chemoreceptors. J Physiol. 1965 Jun;178(3):463–476. doi: 10.1113/jphysiol.1965.sp007637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eyzaguirre C., Zapata P. Pharmacology of pH effects on carotid body chemoreceptors in vitro. J Physiol. 1968 Apr;195(3):557–588. doi: 10.1113/jphysiol.1968.sp008474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eyzaguirre C., Zapata P. The release of acetylcholine from carotid body tissues. Further study on the effects of acetylcholine and cholinergic blocking agents on the chemosensory discharge. J Physiol. 1968 Apr;195(3):589–607. doi: 10.1113/jphysiol.1968.sp008475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones J. V., Ballard K. J. Cholinesterases in the carotid body of the cat as seen with the electron microscope. Nat New Biol. 1971 Sep 29;233(39):146–148. doi: 10.1038/newbio233146a0. [DOI] [PubMed] [Google Scholar]
  11. KOELLE G. B. The elimination of enzymatic diffusion artifacts in the histochemical localization of cholinesterases and a survey of their cellular distributions. J Pharmacol Exp Ther. 1951 Oct;103(2):153–171. [PubMed] [Google Scholar]
  12. KOELLE G. B. The histochemical differentiation of types of cholinesterases and their localizations in tissues of the cat. J Pharmacol Exp Ther. 1950 Oct;100(2):158–179. [PubMed] [Google Scholar]
  13. Kása P., Mann S. P., Hebb C. Localization of choline acetyltransferase. Ultrastructural localization in spinal neurones. Nature. 1970 May 30;226(5248):814–816. doi: 10.1038/226814a0. [DOI] [PubMed] [Google Scholar]
  14. Kása P., Mann S. P., Hebb C. Localization of choline acetyltransferase. Nature. 1970 May 30;226(5248):812–814. doi: 10.1038/226812a0. [DOI] [PubMed] [Google Scholar]
  15. NEIL E. Chemoreceptor areas and chemoreceptor circulatory reflexes. Acta Physiol Scand. 1951 Feb 21;22(1):54–65. doi: 10.1111/j.1748-1716.1951.tb00750.x. [DOI] [PubMed] [Google Scholar]
  16. PALAY S. L., McGEE-RUSSELL S. M., GORDON S., Jr, GRILLO M. A. Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide. J Cell Biol. 1962 Feb;12:385–410. doi: 10.1083/jcb.12.2.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Serafini-Fracassini A., Frasson P. Histochemical observations on the carotid body of the dog. Acta Anat (Basel) 1966;63(2):240–248. doi: 10.1159/000142791. [DOI] [PubMed] [Google Scholar]

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