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. 1972 Feb;221(1):15–27. doi: 10.1113/jphysiol.1972.sp009735

Intracellular pH, H ion flux and H ion permeability coefficient in bullfrog toe muscle

K T Izutsu
PMCID: PMC1331316  PMID: 4536964

Abstract

1. The dimethyloxazolidinedione (DMO) technique was used to estimate intracellular pH (pHi) in bullfrog toe muscles incubated in vitro. The control value of pHi was 7·16 + ± 0·01 (S.D.).

2. pHi was affected by changes in PCO2 and external bicarbonate ion concentration ([HCO3-]0). At a given PCO2, decreasing the external [HCO3-] was more effective in lowering pHi than increasing the external [HCO3-] was in increasing pHi.

3. On the assumption that the changes in pHi were due to hydrogen ion [H+) movements across the membrane, a H+ flux of 10-13 mole/cm2. sec was calculated. The corresponding H+ permeability coefficient was 10-3 cm/sec.

4. The variability of the tissue CO2 buffer value was examined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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