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. 1972 Jun;223(2):461–482. doi: 10.1113/jphysiol.1972.sp009858

The effect of low temperature on the excitation-contraction coupling phenomena of frog single muscle fibres

Carlo Caputo
PMCID: PMC1331458  PMID: 4537710

Abstract

1. Potassium contractures are affected by low temperature: the maximum contracture tension is diminished by about 15% at 3° C, while the response time course is greatly prolonged.

2. The contractile threshold for potassium contractures is lowered by about 10 mV at 3° C.

3. The fibre's membrane is depolarized by approximately the same amount when exposed to solutions with increased potassium concentrations at 20 or 3° C.

4. The repriming process, that is, the process by which the fibres recover their contractile ability following a potassium contracture, proceeds about six times slower at 3° C. This effect is not due to failure of the fibres to repolarize in the cold when transferred from a high potassium to a low potassium medium.

5. At low temperature repolarization occurs, even though it is somewhat slower. Following the solution change, from 190 mM potassium to a low potassium solution, the initial rate of repolarization is 8·5 mV/sec at 20° C, and 3·4 mV/sec at 3° C. This effect is not sufficient to account for the delay in the repriming process.

6. After a potassium contracture, recovery of the fibre's twitching ability at 3° C is also delayed. At a time when twitches have not yet been recovered, membrane potentials of -90 mV and almost normal action potentials can be recorded.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adrian R. H., Chandler W. K., Hodgkin A. L. The kinetics of mechanical activation in frog muscle. J Physiol. 1969 Sep;204(1):207–230. doi: 10.1113/jphysiol.1969.sp008909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BIANCHI C. P., SHANES A. M. Calcium influx in skeletal muscle at rest, during activity, and during potassium contracture. J Gen Physiol. 1959 Mar 20;42(4):803–815. doi: 10.1085/jgp.42.4.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CSAPO A., WILKIE D. R. The dynamics of the effect of potassium on frog's muscle. J Physiol. 1956 Dec 28;134(3):497–514. doi: 10.1113/jphysiol.1956.sp005660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CURTIS B. A. THE RECOVERY OF CONTRACTILE ABILITY FOLLOWING A CONTRACTURE IN SKELETAL MUSCLE. J Gen Physiol. 1964 May;47:953–964. doi: 10.1085/jgp.47.5.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caputo C. Caffeine- and potassium-induced contractures of frog striated muscle fibers in hypertonic solutions. J Gen Physiol. 1966 Sep;50(1):129–139. doi: 10.1085/jgp.50.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Caputo C., Gimenez M. Effects of external calcium deprivation on single muscle fibers. J Gen Physiol. 1967 Oct;50(9):2177–2195. doi: 10.1085/jgp.50.9.2177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Caputo C. The time course of potassium contractures of single muscle fibres. J Physiol. 1972 Jun;223(2):483–505. doi: 10.1113/jphysiol.1972.sp009859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EDWARDS C., CARLSON F. D. POTASSIUM CONTRACTURES AND CREATINE PHOSPHATE BREAKDOWN IN FROG MUSCLE. Biochim Biophys Acta. 1964 Jul 29;88:213–215. doi: 10.1016/0926-6577(64)90170-6. [DOI] [PubMed] [Google Scholar]
  9. Ebashi S., Endo M. Calcium ion and muscle contraction. Prog Biophys Mol Biol. 1968;18:123–183. doi: 10.1016/0079-6107(68)90023-0. [DOI] [PubMed] [Google Scholar]
  10. Frankenhaeuser B., Lännergren J. The effect of calcium on the mechanical response of single twitch muscle fibres of Xenopus laevis. Acta Physiol Scand. 1967 Mar;69(3):242–254. doi: 10.1111/j.1748-1716.1967.tb03518.x. [DOI] [PubMed] [Google Scholar]
  11. HODGKIN A. L., HOROWICZ P. Potassium contractures in single muscle fibres. J Physiol. 1960 Sep;153:386–403. doi: 10.1113/jphysiol.1960.sp006541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HODGKIN A. L., HOROWICZ P. The effect of sudden changes in ionic concentrations on the membrane potential of single muscle fibres. J Physiol. 1960 Sep;153:370–385. doi: 10.1113/jphysiol.1960.sp006540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HODGKIN A. L., HOROWICZ P. The influence of potassium and chloride ions on the membrane potential of single muscle fibres. J Physiol. 1959 Oct;148:127–160. doi: 10.1113/jphysiol.1959.sp006278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jöbsis F. F., Duffield J. C. Oxidative and glycolytic recovery metabolism in muscle. J Gen Physiol. 1967 Mar;50(4):1009–1047. doi: 10.1085/jgp.50.4.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lüttgau H. C., Oetliker H. The action of caffeine on the activation of the contractile mechanism in straited muscle fibres. J Physiol. 1968 Jan;194(1):51–74. doi: 10.1113/jphysiol.1968.sp008394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Milligan J. V., Edwards C. Some factors affecting the time course of the recovery of contracture ability following a potassium contracture in frog striated muscle. J Gen Physiol. 1965 Jul;48(6):975–983. [PMC free article] [PubMed] [Google Scholar]
  17. Nakajima S., Hodgkin A. L. Effect of diameter on the electrical constants of frog skeletal muscle fibres. Nature. 1970 Sep 5;227(5262):1053–1055. doi: 10.1038/2271053a0. [DOI] [PubMed] [Google Scholar]
  18. Winegrad S. The intracellular site of calcium activaton of contraction in frog skeletal muscle. J Gen Physiol. 1970 Jan;55(1):77–88. doi: 10.1085/jgp.55.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]

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