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. 1972 Feb;220(3):529–545. doi: 10.1113/jphysiol.1972.sp009721

Circadian rhythms in human salivary flow rate and composition

C Dawes
PMCID: PMC1331668  PMID: 5016036

Abstract

1. Unstimulated whole saliva and parotid saliva stimulated at a constant flow rate of 1·0 ml./min were collected from eight subjects at about 07.00, 11.00, 14.00, 17.00 and 22.00 hr and oral temperature was recorded several times daily for time spans of between 4 and 26 days. A least-squares cosine wave was fitted to the data to test for the presence and characteristics of circadian rhythms.

2. Estimates of mean level, amplitude, acrophase and period were obtained for different components and the results were subjected to cosinor analysis.

3. Unstimulated whole saliva showed significant circadian rhythms in flow rate and in the concentrations of sodium and chloride but not in protein, potassium, calcium, phosphate or urea.

4. Stimulated parotid saliva showed significant circadian rhythms in the concentrations of protein, sodium, potassium, calcium and chloride but not in phosphate or urea

5. Oral temperature showed a circadian rhythm which, like the salivary rhythms, was of a 24.0 hr periodicity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bissada N. F., Haus E. Circadian rhythms of certain parotid gland functions. Egypt Dent J. 1968 Jul;14(3):125–145. [PubMed] [Google Scholar]
  2. CHAUNCEY H. H., FELLER R. P., SHANNON I. L. Effect of acid solutions on human gustatory chemoreceptors as determined by parotid gland secretion rate. Proc Soc Exp Biol Med. 1963 Apr;112:917–923. doi: 10.3181/00379727-112-28210. [DOI] [PubMed] [Google Scholar]
  3. COULOMBE J. J., FAVREAU L. A new simple semimicro method for colorimetric determination of urea. Clin Chem. 1963 Feb;9:102–108. [PubMed] [Google Scholar]
  4. DE TRAVERSE P. M., COQUELET M. L. Variations nycthémérales du rapport sodium/potassium dans la salive et l'urine. C R Seances Soc Biol Fil. 1952 Jul;146(13-14):1099–1102. [PubMed] [Google Scholar]
  5. Dawes C. The effect of flow rate and length of stimulation on the protein concentration in human parotid saliva. Arch Oral Biol. 1967 Jul;12(7):783–788. doi: 10.1016/0003-9969(67)90101-x. [DOI] [PubMed] [Google Scholar]
  6. Dawes C. The effects of flow rate and duration of stimulation on the condentrations of protein and the main electrolytes in human parotid saliva. Arch Oral Biol. 1969 Mar;14(3):277–294. doi: 10.1016/0003-9969(69)90231-3. [DOI] [PubMed] [Google Scholar]
  7. Dawes C. The secretion of magnesium and calcium in human parotid saliva. Caries Res. 1967;1(4):333–342. doi: 10.1159/000259533. [DOI] [PubMed] [Google Scholar]
  8. FERGUSON M. H., KRAHN K. P., HILDES J. A. Parotid secretion of protein in man. Can J Biochem Physiol. 1958 Oct;36(10):1001–1008. [PubMed] [Google Scholar]
  9. GRAD B. Diurnal, age, and sex changes in the sodium and potassium concentration of human saliva. J Gerontol. 1954 Jul;9(3):276–286. doi: 10.1093/geronj/9.3.276. [DOI] [PubMed] [Google Scholar]
  10. GRAD B. The influence of ACTH on the sodium and potassium concentration of human mixed saliva. J Clin Endocrinol Metab. 1952 Jun;12(6):708–718. doi: 10.1210/jcem-12-6-708. [DOI] [PubMed] [Google Scholar]
  11. HOLMES J. H. CHANGES IN SALIVARY FLOW PRODUCED BY CHANGES IN FLUID AND ELECTROLYTE BALANCE. Int Ser Monogr Oral Biol. 1964;3:177–195. doi: 10.1016/b978-1-4832-2871-6.50019-9. [DOI] [PubMed] [Google Scholar]
  12. Halberg F., Reinhardt J., Bartter F. C., Delea C., Gordon R., Reinberg A., Ghata J., Halhuber M., Hofmann H., Günther R. Agreement in endpoints from circadian rhythmometry on healthy humans beings living on different continents. Experientia. 1969 Jan 15;25(1):107–112. doi: 10.1007/BF01903927. [DOI] [PubMed] [Google Scholar]
  13. Henkin R. I., Gill J. R., Bartter F. C. STUDIES ON TASTE THRESHOLDS IN NORMAL MAN AND IN PATIENTS WITH ADRENAL CORTICAL INSUFFICIENCY: THE ROLE OF ADRENAL CORTICAL STEROIDS AND OF SERUM SODIUM CONCENTRATION. J Clin Invest. 1963 May;42(5):727–735. doi: 10.1172/JCI104765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Junqueira L. C., Fava-De-Moraes F., Toledo M. S. Action of vasopressin on salivary secretion. Acta Physiol Lat Am. 1967;17(1):36–41. [PubMed] [Google Scholar]
  15. KRAL V. A., GRAD B., HUNZINGER W. Diurnal variation patterns of circulating eosinophil counts and salivary Na/K in psychiatric patients. J Nerv Ment Dis. 1959 Jul;129:69–75. doi: 10.1097/00005053-195907000-00008. [DOI] [PubMed] [Google Scholar]
  16. Katz F. H., Shannon I. L. Parotid fluid cortisol and cortisone. J Clin Invest. 1969 May;48(5):848–855. doi: 10.1172/JCI106042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kleinberg I. Formation and accumulation of acid on the tooth surface. J Dent Res. 1970 Nov-Dec;49(6):1300–1317. doi: 10.1177/00220345700490062301. [DOI] [PubMed] [Google Scholar]
  18. Mangos J. A., McSherry N. R. Micropuncture study of sodium and potassium excretion in rat parotid saliva: role of aldosterone. Proc Soc Exp Biol Med. 1969 Nov;132(2):797–801. doi: 10.3181/00379727-132-34311. [DOI] [PubMed] [Google Scholar]
  19. Mills J. N. Human circadian rhythms. Physiol Rev. 1966 Jan;46(1):128–171. doi: 10.1152/physrev.1966.46.1.128. [DOI] [PubMed] [Google Scholar]
  20. PALMAI G., BLACKWELL B. THE DIURNAL PATTERN OF SALIVARY FLOW IN NORMAL AND DEPRESSED PATIENTS. Br J Psychiatry. 1965 Apr;111:334–338. doi: 10.1192/bjp.111.473.334. [DOI] [PubMed] [Google Scholar]
  21. PAWAN G. L. Studies on the salivary sodium potassium ratio in man. Biochem J. 1955 Mar 19;60(ANNUAL):xii–xii. [PubMed] [Google Scholar]
  22. PECK R. E. The SHP test--an aid in the detection and measurement of depression. AMA Arch Gen Psychiatry. 1959 Jul;1:35–40. doi: 10.1001/archpsyc.1959.03590010051006. [DOI] [PubMed] [Google Scholar]
  23. SCHNEYER L. H., PIGMAN W., HANAHAN L., GILMORE R. W. Rate of flow of human parotid, sublingual, and submaxillary secretions during sleep. J Dent Res. 1956 Feb;35(1):109–114. doi: 10.1177/00220345560350010301. [DOI] [PubMed] [Google Scholar]
  24. Shannon I. L., Chauncey H. H. A parotid fluid collection device with improved stability characteristics. J Oral Ther Pharmacol. 1967 Sep;4(2):93–97. [PubMed] [Google Scholar]
  25. Shannon I. L., Suddick R. P., Chauncey H. H. Effect of atropine-induced flow rate depression on the composition of unstimulated human parotid fluid. Arch Oral Biol. 1969 Jul;14(7):761–770. doi: 10.1016/0003-9969(69)90167-8. [DOI] [PubMed] [Google Scholar]
  26. WESSON L. G., Jr ELECTROLYTE EXCRETION IN RELATION TO DIURNAL CYCLES OF RENAL FUNCTION. Medicine (Baltimore) 1964 Sep;43:547–592. doi: 10.1097/00005792-196409000-00002. [DOI] [PubMed] [Google Scholar]
  27. Windeler A. S., Jr, Shannon I. L. The effect of flow rate on parotid fluid calcium and protein levels. Arch Oral Biol. 1966 Oct;11(10):1043–1045. doi: 10.1016/0003-9969(66)90205-6. [DOI] [PubMed] [Google Scholar]
  28. Wolfe L. K., Gordon R. D., Island D. P., Liddle G. W. An analysis of factors determining the circadian pattern of aldosterone excretion. J Clin Endocrinol Metab. 1966 Nov;26(11):1261–1266. doi: 10.1210/jcem-26-11-1261. [DOI] [PubMed] [Google Scholar]

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