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. 1971 Feb;213(1):85–93. doi: 10.1113/jphysiol.1971.sp009369

The frequency dependent character of the membrane capacity in cardiac Purkyně fibres

E Carmeliet, J Willems
PMCID: PMC1331724  PMID: 5575345

Abstract

1. The upstroke of the action potential was used as a signal of variable frequency in order to measure the apparent membrane capacity of cardiac Purkyně fibres.

2. The upstroke in Na-Tyrode can be regarded as a high frequency signal. Action potentials in Na-free, Sr-Tyrode are characterized by a very small rate of depolarization (5 V/sec) and conduction velocity (15 cm/sec) and were used as a low frequency signal.

3. On changing from Na- to Sr-Tyrode the apparent membrane capacity increased by a factor of 3·77 and approached the value estimated by the square-current technique.

4. Cable properties (space and time constants, internal core and membrane resistance) were found to be constant when changing from Na- to Sr-Tyrode.

5. The significance of the frequency dependent character of the membrane capacity is discussed in relation to propagation and form of the action potential.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beeler G. W., Jr, Reuter H. Voltage clamp experiments on ventricular myocarial fibres. J Physiol. 1970 Mar;207(1):165–190. doi: 10.1113/jphysiol.1970.sp009055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carmeliet E., Van Bogaert P. P. Strontium action potentials in cardiac Purkyne fibers. Arch Int Physiol Biochim. 1969 Feb;77(1):134–135. [PubMed] [Google Scholar]
  3. Dudel J., Peper K., Rüdel R., Trautwein W. Excitatory membrane current in heart muscle (Purkinje fibers). Pflugers Arch Gesamte Physiol Menschen Tiere. 1966;292(3):255–273. doi: 10.1007/BF00362740. [DOI] [PubMed] [Google Scholar]
  4. FALK G., FATT P. LINEAR ELECTRICAL PROPERTIES OF STRIATED MUSCLE FIBRES OBSERVED WITH INTRACELLULAR ELECTRODES. Proc R Soc Lond B Biol Sci. 1964 Apr 14;160:69–123. doi: 10.1098/rspb.1964.0030. [DOI] [PubMed] [Google Scholar]
  5. Fozzard H. A. Membrane capacity of the cardiac Purkinje fibre. J Physiol. 1966 Jan;182(2):255–267. doi: 10.1113/jphysiol.1966.sp007823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Freygang W. H., Jr, Rapoport S. I., Peachey L. D. Some relations between changes in the linear electrical properties of striated muscle fibers and changes in ultrastructure. J Gen Physiol. 1967 Nov;50(10):2437–2458. doi: 10.1085/jgp.50.10.2437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Freygang W. H., Trautwein W. The structural implications of the linear electrical properties of cardiac Purkinje strands. J Gen Physiol. 1970 Apr;55(4):524–547. doi: 10.1085/jgp.55.4.524. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gage P. W., Eisenberg R. S. Capacitance of the surface and transverse tubular membrane of frog sartorius muscle fibers. J Gen Physiol. 1969 Mar;53(3):265–278. doi: 10.1085/jgp.53.3.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McAllister R. E. Computed action potentials for Purkinje fiber membranes with resistance and capacitance in series. Biophys J. 1968 Aug;8(8):951–964. doi: 10.1016/S0006-3495(68)86530-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reuter H. The dependence of slow inward current in Purkinje fibres on the extracellular calcium-concentration. J Physiol. 1967 Sep;192(2):479–492. doi: 10.1113/jphysiol.1967.sp008310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sakamoto Y. Membrane characteristics of the canine papillary muscle fiber. J Gen Physiol. 1969 Dec;54(6):765–781. doi: 10.1085/jgp.54.6.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sommer J. R., Johnson E. A. Cardiac muscle. A comparative study of Purkinje fibers and ventricular fibers. J Cell Biol. 1968 Mar;36(3):497–526. doi: 10.1083/jcb.36.3.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TASAKI I., HAGIWARA S. Capacity of muscle fiber membrane. Am J Physiol. 1957 Mar;188(3):423–429. doi: 10.1152/ajplegacy.1957.188.3.423. [DOI] [PubMed] [Google Scholar]
  14. WEIDMANN S. The electrical constants of Purkinje fibres. J Physiol. 1952 Nov;118(3):348–360. doi: 10.1113/jphysiol.1952.sp004799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Willems J., Carmeliet E. E. Membrane capacity of cardiac Purkyne fibre in sodium- and strontium-Tyrode. Arch Int Physiol Biochim. 1969 Dec;77(5):943–944. [PubMed] [Google Scholar]

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