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. 1971 Sep;217(2):259–279. doi: 10.1113/jphysiol.1971.sp009570

Vagal non-adrenergic inhibition of guinea-pig stomach

L Beani, Clementina Bianchi, A Crema
PMCID: PMC1331776  PMID: 4398576

Abstract

1. The effect of vagal and sympathetic stimulation on the mechanical and electrical activity (intracellular recording) of the body of the guinea-pig stomach was investigated in vitro.

2. Following atropine, 1 × 10-6-1 × 10-7 g/ml., vagal responses were reversed from excitatory to inhibitory.

3. Sympathetic blockade, produced by α- and β-receptor antagonists and adrenergic neurone-blocking agents, reduced or abolished sympathetic, but not vagal inhibition.

4. Hexamethonium (5 × 10-5 g/ml.) reduced vagal relaxation to 11-30% according to the stimulation rate. The residual response was maintained in the presence of 5-hydroxytryptamine tachyphylaxis.

5. Many muscle cells were observed to be under the influence of both vagus and sympathetic nerves: the effect of sympathetic stimulation was always inhibitory in nature, but high stimulation rates were required. The effect of vagal stimulation was both excitatory and inhibitory even in the absence of atropine: low stimulation rates gave rise either to E.J.P.s (excitatory junctional potentials), often followed by spikes, or to I.J.P.s (inhibitory junctional potentials).

6. In some spontaneously firing cells the interruption of electrical activity produced by vagal stimulation at 2/sec and sympathetic stimulation at 20/sec was recorded for a long enough time to check the effect of guanethedine (5 × 10-6 g/ml.): the drug selectively blocked sympathetic inhibition.

7. The significance of the inhibitory non-adrenergic transmitter, released by the intramural neurones activated by preganglionic vagal fibres, is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrahamsson H., Jansson G. Elicitation of reflex vagal relaxation of the stomach from pharynx and esophagus in the cat. Acta Physiol Scand. 1969 Sep-Oct;77(1):172–178. doi: 10.1111/j.1748-1716.1969.tb04561.x. [DOI] [PubMed] [Google Scholar]
  2. BOURA A. L., GREEN A. F. The actions of bretylium: adrenergic neurone blocking and other effects. Br J Pharmacol Chemother. 1959 Dec;14:536–548. doi: 10.1111/j.1476-5381.1959.tb00961.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BURNSTOCK G., CAMPBELL G., BENNETT M., HOLMAN M. E. INHIBITION OF THE SMOOTH MUSCLE ON THE TAENIA COLI. Nature. 1963 Nov 9;200:581–582. doi: 10.1038/200581a0. [DOI] [PubMed] [Google Scholar]
  4. BURNSTOCK G., CAMPBELL G., BENNETT M., HOLMAN M. E. INNERVATION OF THE GUINEA-PIG TAENIA COLI: ARE THERE INTRINSIC INHIBITORY NERVES WHICH ARE DISTINCT FROM SYMPATHETIC NERVES? Int J Neuropharmacol. 1964 May;3:163–166. doi: 10.1016/0028-3908(64)90003-6. [DOI] [PubMed] [Google Scholar]
  5. Bennett M. R., Burnstock G., Holman M. Transmission from intramural inhibitory nerves to the smooth muscle of the guinea-pig taenia coli. J Physiol. 1966 Feb;182(3):541–558. doi: 10.1113/jphysiol.1966.sp007836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bennett M. R. Rebound excitation of the smooth muscle cells of the guinea-pig taenia coli after stimulation of intramural inhibitory nerves. J Physiol. 1966 Jul;185(1):124–131. doi: 10.1113/jphysiol.1966.sp007975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bianchi C., Beani L., Crema C. Effects of metoclopramide on isolated guinea-pig colon. 2. Interference with ganglionic stimulant drugs. Eur J Pharmacol. 1970;12(3):332–341. doi: 10.1016/0014-2999(70)90085-3. [DOI] [PubMed] [Google Scholar]
  8. Bianchi C., Beani L., Frigo G. M., Crema A. Further evidence for the presence of non-adrenergic inhibitory structures in the guinea-pig colon. Eur J Pharmacol. 1968 Aug;4(1):51–61. doi: 10.1016/0014-2999(68)90009-5. [DOI] [PubMed] [Google Scholar]
  9. Burnstock G., Campbell G., Rand M. J. The inhibitory innervation of the taenia of the guinea-pig caecum. J Physiol. 1966 Feb;182(3):504–526. doi: 10.1113/jphysiol.1966.sp007834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Bülbring E., Gershon M. D. 5-hydroxytryptamine participation in the vagal inhibitory innervation of the stomach. J Physiol. 1967 Oct;192(3):823–846. doi: 10.1113/jphysiol.1967.sp008334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Bülbring E., Tomita T. Properties of the inhibitory potential of smooth muscle as observed in the response to field stimulation of the guinea-pig taenia coli. J Physiol. 1967 Apr;189(2):299–315. doi: 10.1113/jphysiol.1967.sp008169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Campbell G. Nerve-mediated excitation of the taenia of the guinea-pig caecum. J Physiol. 1966 Jul;185(1):148–159. doi: 10.1113/jphysiol.1966.sp007977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Campbell G. The inhibitory nerve fibres in the vagal supply to the guinea-pig stomach. J Physiol. 1966 Aug;185(3):600–612. doi: 10.1113/jphysiol.1966.sp008004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. EVANS D. H., MURRAY J. G. Histological and functional studies on the fibre composition of the vagus nerve of the rabbit. J Anat. 1954 Jul;88(3):320–337. [PMC free article] [PubMed] [Google Scholar]
  15. Furness J. B. An electrophysiological study of the innervation of the smooth muscle of the colon. J Physiol. 1969 Dec;205(3):549–562. doi: 10.1113/jphysiol.1969.sp008982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Furness J. B. An examination of nerve-mediated, hyoscine-resistant excitation of the guinea-pig colon. J Physiol. 1970 May;207(3):803–821. doi: 10.1113/jphysiol.1970.sp009096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. GARRY R. C., GILLESPIE J. S. The responses of the musculature of the colon of the rabbit to stimulation, in vitro, of the parasympathetic and of the sympathetic outflows. J Physiol. 1955 Jun 28;128(3):557–576. doi: 10.1113/jphysiol.1955.sp005325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. HOLMAN M. E., HUGHES J. R. INHIBITION OF INTESTINAL SMOOTH MUSCLE. Aust J Exp Biol Med Sci. 1965 Jun;43:277–290. doi: 10.1038/icb.1965.27. [DOI] [PubMed] [Google Scholar]
  19. HUKOVIC S. The action of sympathetic blocking agents on isolated and innervated atria and vessels. Br J Pharmacol Chemother. 1960 Mar;15:117–121. doi: 10.1111/j.1476-5381.1960.tb01218.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Jansson G. Vago-vagal reflex relaxation of the stomach in the cat. Acta Physiol Scand. 1969 Jan-Feb;75(1):245–252. doi: 10.1111/j.1748-1716.1969.tb04376.x. [DOI] [PubMed] [Google Scholar]
  21. Kuriyama H., Osa T., Tasaki H. Electrophysiological studies of the antrum muscle fibers of the guinea pig stomach. J Gen Physiol. 1970 Jan;55(1):48–62. doi: 10.1085/jgp.55.1.48. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Martinson J. Vagal relaxation of the stomach. Experimental re-investigation of the concept of the transmission mechanism. Acta Physiol Scand. 1965 Aug;64(4):453–462. doi: 10.1111/j.1748-1716.1965.tb04204.x. [DOI] [PubMed] [Google Scholar]
  23. McSwiney B. A., Robson J. M. The response of smooth muscle to stimulation of the vagus nerve. J Physiol. 1929 Oct 23;68(2):124–131. doi: 10.1113/jphysiol.1929.sp002599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. PATON W. D., VANE J. R. Analysis of he responses of the isolated stomach to electrical stimulation and to drugs. J Physiol. 1963 Jan;165:10–46. doi: 10.1113/jphysiol.1963.sp007040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WOODBURY J. W., BRADY A. J. Intracellular recording from moving tissues with a flexibly mounted ultramicroelectrode. Science. 1956 Jan 20;123(3186):100–101. doi: 10.1126/science.123.3186.100-a. [DOI] [PubMed] [Google Scholar]

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