Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1971 May;214(3):583–597. doi: 10.1113/jphysiol.1971.sp009450

The transfer of free α-amino nitrogen across the placental membrane in the guinea-pig

Margaret L Reynolds, Maureen Young
PMCID: PMC1331856  PMID: 5104038

Abstract

1. Transfer of endogenous α-amino nitrogen from the maternal to foetal side of the placental membrane was studied in the foetal guinea-pig placenta perfused, in situ via the umbilical vessels, with an artificial fluid. Changes in maternal placental blood flow were followed by measuring the transfer of antipyrine into the perfusate.

2. Active transport by the membrane was demonstrated under these conditions by observing the increased ratio of L/D leucine appearing in the perfusate in comparison with the maternal plasma ratio.

3. Reduction in maternal placental flow following haemorrhage was associated with a fall in transfer of amino nitrogen to about half of the control values.

4. The concentration of α-amino nitrogen in the perfusate was inversely proportional to the foetal placental flow which had a smaller influence on amino nitrogen transfer per min than did the maternal blood flow.

5. Hypoxia and some inhibitors of oxidative metabolism were without influence on the transfer of α-amino nitrogen in this preparation.

Full text

PDF
583

Images in this article

587Fig. 1
on p.587

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRADLEY R. D. DIAGNOSTIC RIGHT-HEART CATHETERISATION WITH MINIATURE CATHETERS IN SEVERELY ILL PATIENTS. Lancet. 1964 Oct 31;2(7366):941–942. doi: 10.1016/s0140-6736(64)90869-4. [DOI] [PubMed] [Google Scholar]
  2. BROWNE J. C. M., VEALL N. The maternal placental blood flow in normotensive and hypertensive women. J Obstet Gynaecol Br Emp. 1953 Apr;60(2):141–147. doi: 10.1111/j.1471-0528.1953.tb07668.x. [DOI] [PubMed] [Google Scholar]
  3. Bell G. H. The behaviour of the pregnant uterus of the guinea pig. J Physiol. 1941 Nov 28;100(3):263–274. doi: 10.1113/jphysiol.1941.sp003941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell A. G., Dawes G. S., Fishman A. P., Hyman A. I., James G. B. The oxygen consumption of the placenta and foetal membranes in the sheep. J Physiol. 1966 Jan;182(2):439–464. doi: 10.1113/jphysiol.1966.sp007831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DANCIS J., BRENNER M. A., MONEY W. L. Some factors affecting the permeability of guinea pig placenta. Am J Obstet Gynecol. 1962 Sep 1;84:570–576. doi: 10.1016/0002-9378(62)90145-x. [DOI] [PubMed] [Google Scholar]
  6. DANCIS J., OLSEN G., FOLKART G. Transfer of histidine and xylose across the placenta and into the red blood cell and amniotic fluids. Am J Physiol. 1958 Jul;194(1):44–46. doi: 10.1152/ajplegacy.1958.194.1.44. [DOI] [PubMed] [Google Scholar]
  7. DAWES G. S. The umbilical circulation. Am J Obstet Gynecol. 1962 Dec 1;84:1634–1648. doi: 10.1016/0002-9378(62)90007-8. [DOI] [PubMed] [Google Scholar]
  8. Dancis J., Money W. L., Springer D., Levitz M. Transport of amino acids by placenta. Am J Obstet Gynecol. 1968 Jul 15;101(6):820–829. doi: 10.1016/0002-9378(68)90038-0. [DOI] [PubMed] [Google Scholar]
  9. Ely P. A. The placental transfer of hexoses and polyols in the guinea-pig, as shown by umbilical perfusion of the placenta. J Physiol. 1966 May;184(2):255–271. doi: 10.1113/jphysiol.1966.sp007914. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gaddum J. H., Peart W. S., Vogt M. The estimation of adrenaline and allied substances in blood. J Physiol. 1949 Jun 15;108(4):467–481. doi: 10.1113/jphysiol.1949.sp004350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hey E. N. A small heated respiratory valve. J Physiol. 1966 Oct;186(2):49P–51P. [PubMed] [Google Scholar]
  12. Hopins L., Reynolds M. L., Young M. The influence of blood flow and hypoxia on free alpha-amino nitrogen transfer across the placental membrane. J Physiol. 1970 Mar;207(1):13P–15P. [PubMed] [Google Scholar]
  13. JACOBS F. A., LANG A. H. DYNAMICS OF AMINO ACID TRANSPORT IN THE INTACT INTESTINE. Proc Soc Exp Biol Med. 1965 Mar;118:772–776. doi: 10.3181/00379727-118-29966. [DOI] [PubMed] [Google Scholar]
  14. KARVONEN M. J., LAAMANEN A., RAIHA N. Lack of effect of oxygen tension upon the permeability of the guinea pig placenta to d(+) xylose and sorbose. Acta Physiol Scand. 1956 May 18;36(3):245–249. doi: 10.1111/j.1748-1716.1956.tb01321.x. [DOI] [PubMed] [Google Scholar]
  15. Lucas W. E., Kirschbaum T. H., Assali N. S. Effects of autonomic blockade with spinal anesthesia on uterine and fetal hemodynamics and oxygen consumption in the sheep. Biol Neonat. 1966;10(3):166–169. doi: 10.1159/000240026. [DOI] [PubMed] [Google Scholar]
  16. Lumley J. M., Wood C. Influence of hypoxia on glucose transport across the human placenta. Nature. 1967 Oct 28;216(5113):403–404. doi: 10.1038/216403a0. [DOI] [PubMed] [Google Scholar]
  17. MONEY W. L., DANCIS J. Technique for the in situ study of placental transport in the pregnant guinea pig. Am J Obstet Gynecol. 1960 Aug;80:209–214. doi: 10.1016/0002-9378(60)90114-9. [DOI] [PubMed] [Google Scholar]
  18. OXENDER D. L., CHRISTENSEN H. N. Transcellular concentration as a consequence of intracellular accumulation. J Biol Chem. 1959 Sep;234:2321–2324. [PubMed] [Google Scholar]
  19. PAGE E. W., GLENDENING M. B., MARGOLIS A., HARPER H. A. Transfer of D- and L-histidine across the human placenta. Am J Obstet Gynecol. 1957 Mar;73(3):589-94; discussion, 594-7. doi: 10.1016/s0002-9378(16)37431-2. [DOI] [PubMed] [Google Scholar]
  20. Parsons D. S., Prichard J. S. A preparation of perfused small intestine for the study of absorption in amphibia. J Physiol. 1968 Sep;198(2):405–434. doi: 10.1113/jphysiol.1968.sp008614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SETNIKAR I. Trasporto di sostanze azotate dalla madre al feto. Boll Soc Ital Biol Sper. 1957 Oct-Nov;33(10-11):1636–1640. [PubMed] [Google Scholar]
  22. SHEPHERD J. T., WHELAN R. F. The blood flow in the umbilical cord of the foetal guinea-pig. J Physiol. 1951 Oct 29;115(2):150–157. doi: 10.1113/jphysiol.1951.sp004659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. WHITTAM R. Sodium and potassium movements in kidney cortex slices from new-born animals. J Physiol. 1960 Sep;153:358–369. doi: 10.1113/jphysiol.1960.sp006539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WILSON T. H., LIN E. C. Active transport by intestines of fetal and newborn rabbits. Am J Physiol. 1960 Dec;199:1030–1032. doi: 10.1152/ajplegacy.1960.199.6.1030. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES