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. 1971 May;215(1):95–102. doi: 10.1113/jphysiol.1971.sp009459

The maintenance of resting potentials in glycerol-treated muscle fibres

R S Eisenberg, J N Howell, P C Vaughan
PMCID: PMC1331868  PMID: 5579685

Abstract

1. A modification of a previously published method for the disruption of the T-tubules of frog skeletal muscle is described. The modification permits the disruption of the T-tubules without the decline in resting potentials which was reported previously.

2. The method for the disruption of the T-tubules involves the washout of glycerol following loading in a 400 mM glycerol Ringer solution. The modification consists of elevating the concentration of divalent cations in the Ringer used for glycerol washout.

3. The optimum concentrations are 5 mM-Ca2+ and 5 mM-Mg2+ added as their chloride salts. Neither 10 mM-Ca2+ nor 10 mM-Mg2+ are as effective as the combination of each at 5 mM. Other concentrations gave less satisfactory results.

4. The use of the modified technique provides a preparation which maintains 85-90 mV resting potentials for up to 6 or 8 hr but which will not contract in response to membrane depolarization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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