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. 1971 Aug;216(3):611–624. doi: 10.1113/jphysiol.1971.sp009543

Stimulation of amylase secretion from the perfused cat pancreas by potassium and other alkali metal ions

B E Argent, R M Case, T Scratcherd
PMCID: PMC1331925  PMID: 5565641

Abstract

1. In the isolated cat pancreas, stimulated maximally with secretin, increasing the perfusate potassium concentration (at the expense of sodium ions) caused a copious secretion of amylase from the gland, reduced the volume rate of secretion and caused vasoconstriction.

2. Rubidium and caesium had similar effects to potassium: lithium, though depressing secretory rate, had no effect on enzyme secretion or vasoconstrictor action.

3. Amylase secretion was detected at potassium concentrations of 30 mM and was maximal at 80-90 mM, output declining as the concentration was raised to 120 mM.

4. Amylase secretion was maximal during the first few minutes of exposure to excess potassium, but remained above basal levels throughout the test period. Secretory rate was depressed by a constant amount during the test period.

5. Atropine sulphate blocked the effect on enzyme secretion without affecting the reduction in secretory rate.

6. During perfusion with excess potassium a vasodepressor material with the properties of acetylcholine was detected in the effluent from the gland.

7. The reduction in secretory rate, when perfusate sodium was replaced by potassium, was equal to that obtained when sodium was replaced by sucrose.

8. It is concluded that potassium stimulates amylase secretion indirectly by releasing acetylcholine from nerve terminals in the gland, and that the reduction in secretory rate is due not to excess potassium but to sodium deficiency.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argent B. E., Case R. M., Poole L. M., Scratcherd T. Stimulation of enzyme secretion from the perfused cat pancreas by potassium. J Physiol. 1970 Jun;208(2):79P–80P. [PubMed] [Google Scholar]
  2. BDOLAH A., BEN-ZVI R., SCHRAMM M. THE MECHANISM OF ENZYME SECRETION BY THE CELL. II. SECRETION OF AMYLASE AND OTHER PROTEINS BY SLICES OF RAT PAROTID GLAND. Arch Biochem Biophys. 1964 Jan;104:58–66. doi: 10.1016/s0003-9861(64)80034-5. [DOI] [PubMed] [Google Scholar]
  3. Beznák A. B. On the mechanism of the autacoid function of parasympathetic nerves. J Physiol. 1934 Sep 19;82(2):129–153. doi: 10.1113/jphysiol.1934.sp003167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown G. L., Feldberg W. The action of potassium on the superior cervical ganglion of the cat. J Physiol. 1936 Mar 9;86(3):290–305. doi: 10.1113/jphysiol.1936.sp003364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown J. C., Harper A. A., Scratcherd T. Potentiation of secretin stimulation of the pancreas. J Physiol. 1967 Jun;190(3):519–530. doi: 10.1113/jphysiol.1967.sp008225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COUPLAND R. E. The innervation of pan creas of the rat, cat and rabbit as revealed by the cholinesterase technique. J Anat. 1958 Jan;92(1):143–149. [PMC free article] [PubMed] [Google Scholar]
  7. CRICK J., HARPER A. A., RAPER H. S. On the preparation of secretin and pancreozymin. J Physiol. 1949 Dec;110(3-4):367–376. doi: 10.1113/jphysiol.1949.sp004445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Case R. M., Harper A. A., Scratcherd T. Water and electrolyte secretion by the perfused pancreas of the cat. J Physiol. 1968 May;196(1):133–149. doi: 10.1113/jphysiol.1968.sp008499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Collier B. The preferential release of newly synthesized transmitter by a sympathetic ganglion. J Physiol. 1969 Nov;205(2):341–352. doi: 10.1113/jphysiol.1969.sp008969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DOUGLAS W. W., POISNER A. M. STIMULUS-SECRETION COUPLING IN A NEUROSECRETORY ORGAN: THE ROLE OF CALCIUM IN THE RELEASE OF VASOPRESSIN FROM THE NEUROHYPOPHYSIS. J Physiol. 1964 Jul;172:1–18. doi: 10.1113/jphysiol.1964.sp007399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. DOUGLAS W. W., RUBIN R. P. The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J Physiol. 1961 Nov;159:40–57. doi: 10.1113/jphysiol.1961.sp006791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Douglas W. W. Stimulus-secretion coupling: the concept and clues from chromaffin and other cells. Br J Pharmacol. 1968 Nov;34(3):451–474. doi: 10.1111/j.1476-5381.1968.tb08474.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Feldberg W., Guimarāis J. A. The liberation of acetylcholine by potassium. J Physiol. 1936 Mar 9;86(3):306–314. doi: 10.1113/jphysiol.1936.sp003365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Findlay J. A., Gill J. R., Lever J. D., Randle P. J., Spriggs T. L. Increased insulin output following stimulation of the vagal supply to the perfused rabbit pancreas. J Anat. 1969 May;104(Pt 3):580–580. [PubMed] [Google Scholar]
  15. Grodsky G. M., Bennett L. L. Cation requirements for insulin secretion in the isolated perfused pancreas. Diabetes. 1966 Dec;15(12):910–913. doi: 10.2337/diab.15.12.910. [DOI] [PubMed] [Google Scholar]
  16. Hales C. N., Milner R. D. The role of sodium and potassium in insulin secretion from rabbit pancreas. J Physiol. 1968 Feb;194(3):725–743. doi: 10.1113/jphysiol.1968.sp008433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jutisz M., Paloma de la Llosa M. Requirement of Ca++ and Mg++ ions for the in vitro release of follicle-stimulating hormone from rat pituitary glands and in its subsequent biosynthesis. Endocrinology. 1970 Apr;86(4):761–768. doi: 10.1210/endo-86-4-761. [DOI] [PubMed] [Google Scholar]
  18. Lambert A. E., Jeanrenaud B., Junod A., Renold A. E. Organ culture of fetal rat pancreas. II. Insulin release induced by amino and organic acids, by hormonal peptides, by cationic alterations of the medium and by other agents. Biochim Biophys Acta. 1969 Sep 2;184(3):540–553. doi: 10.1016/0304-4165(69)90268-2. [DOI] [PubMed] [Google Scholar]
  19. MacLeod R. M., Fontham E. H. Influence of ionic environment on the in vitro synthesis and release of pituitary hormones. Endocrinology. 1970 Apr;86(4):863–869. doi: 10.1210/endo-86-4-863. [DOI] [PubMed] [Google Scholar]
  20. Malaisse W., Malaisse-Lagae F., Wright P. H., Ashmore J. Effects of adrenergic and cholinergic agents upon insulin secretion in vitro. Endocrinology. 1967 May;80(5):975–978. doi: 10.1210/endo-80-5-975. [DOI] [PubMed] [Google Scholar]
  21. Mann P. J., Tennenbaum M., Quastel J. H. Acetylcholine metabolism in the central nervous system: The effects of potassium and other cations on acetylcholine liberation. Biochem J. 1939 May;33(5):822–835. doi: 10.1042/bj0330822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rasmussen H., Tenenhouse A. Cyclic adenosine monophosphate, CA++, and membranes. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1364–1370. doi: 10.1073/pnas.59.4.1364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Samli M. H., Geschwind I. I. Some effects of energy-transfer inhibitors and of Ca++-free or K+-enhanced media on the release of luteinizing hormone (LH) from the rat pituitary gland in vitro. Endocrinology. 1968 Feb;82(2):225–231. doi: 10.1210/endo-82-2-225. [DOI] [PubMed] [Google Scholar]
  24. Schramm M. Amylase secretion in rat parotid slices by apparent activation of endogenous catecholamine. Biochim Biophys Acta. 1968 Oct 15;165(3):546–549. doi: 10.1016/0304-4165(68)90238-9. [DOI] [PubMed] [Google Scholar]
  25. VOGT M. The secretion of the denervated adrenal medulla of the cat. Br J Pharmacol Chemother. 1952 Jun;7(2):325–330. doi: 10.1111/j.1476-5381.1952.tb01329.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Vale W., Guillemin R. Potassium-induced stimulation of thyrotropin release in vitro. Requirement for presence of calcium and inhibition by thyroxine. Experientia. 1967 Oct 15;23(10):855–857. doi: 10.1007/BF02146888. [DOI] [PubMed] [Google Scholar]

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