Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1971 Jul;216(1):201–211. doi: 10.1113/jphysiol.1971.sp009518

The accumulation of [14C]5-hydroxyindol-3-ylacetic acid by the rabbit choroid plexus in vitro

I A Pullar
PMCID: PMC1331969  PMID: 5558354

Abstract

1. The lateral and fourth ventricular choroid plexuses of the rabbit have been shown to be capable of the in vitro accumulation of 5-hydroxyindol-3-ylacetic acid (5-HIAA).

2. The accumulation of 5-HIAA occurs against a concentration gradient and is dependent on metabolic energy.

3. It is possible to inhibit the accumulation of 5-HIAA by the presence of the dopamine metabolite, 3-methoxy-4-hydroxyphenylacetic acid (HVA) or of probenecid in the incubation medium.

4. It would appear that the characteristics of the accumulation of 5-HIAA by the isolated choroid plexuses are consistent with the involvement of this tissue in the in vivo active transfer of 5-HIAA from the c.s.f. to the blood.

Full text

PDF
201

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDEN N. E., ROOS B. E., WERDINIUS B. ON THE OCCURRENCE OF HOMOVANILLIC ACID AND 3-METHOXY-4-HYDROXYMANDELIC ACID IN HUMAN CEREBROSPINAL FLUID. Experientia. 1963 Jul 15;19:359–360. doi: 10.1007/BF02152317. [DOI] [PubMed] [Google Scholar]
  2. Ashcroft G. W., Dow R. C., Moir A. T. The active transport of 5-hydroxyindol-3-ylacetic acid and 3-methoxy-4-hydroxyphenylacetic acid from a recirculatory perfusion system of the cerebral ventricles of the unanaesthetized dog. J Physiol. 1968 Dec;199(2):397–425. doi: 10.1113/jphysiol.1968.sp008660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CSAKY T. Z., RIGOR B. M., Sr A CONCENTRATIVE MECHANISM FOR SUGARS IN THE CHOROID PLEXUS. Life Sci. 1964 Sep;3:931–936. doi: 10.1016/0024-3205(64)90101-8. [DOI] [PubMed] [Google Scholar]
  4. DAVSON H., KLEEMAN C. R., LEVIN E. Quantitative studies of the passage of different substances out of the cerebrospinal fluid. J Physiol. 1962 Apr;161:126–142. doi: 10.1113/jphysiol.1962.sp006877. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DESPOPOULOS A., WEISSBACH H. Renal metabolism of 5-hydroxyindolacetic acid. Am J Physiol. 1957 Jun;189(3):548–550. doi: 10.1152/ajplegacy.1957.189.3.548. [DOI] [PubMed] [Google Scholar]
  6. Hug C. C., Jr Transport of narcotic analgesics by choroid plexus and kidney tissue in vitro. Biochem Pharmacol. 1967 Feb;16(2):345–359. doi: 10.1016/0006-2952(67)90035-4. [DOI] [PubMed] [Google Scholar]
  7. Lorenzo A. V., Cutler R. W. Amino acid transport by choroid plexus in vitro. J Neurochem. 1969 Apr;16(4):577–585. doi: 10.1111/j.1471-4159.1969.tb06857.x. [DOI] [PubMed] [Google Scholar]
  8. PAPPENHEIMER J. R., HEISEY S. R., JORDAN E. F. Active transport of Diodrast and phenolsulfonphthalein from cerebrospinal fluid to blood. Am J Physiol. 1961 Jan;200:1–10. doi: 10.1152/ajplegacy.1961.200.1.1. [DOI] [PubMed] [Google Scholar]
  9. POLLAY M., DAVSON H. The passage of certain substances out of the cerebrosphinal fluid. Brain. 1963 Mar;86:137–150. doi: 10.1093/brain/86.1.137. [DOI] [PubMed] [Google Scholar]
  10. Robinson R. J., Cutler R. W., Lorenzo A. V., Barlow C. F. Transport of sulphate, thiosulphate and iodide by choroid plexus in vitro. J Neurochem. 1968 Oct;15(10):1169–1179. doi: 10.1111/j.1471-4159.1968.tb06834.x. [DOI] [PubMed] [Google Scholar]
  11. TOCHINO Y., SCHANKER L. S. ACTIVE TRANSPORT OF QUATERNARY AMMONIUM COMPOUNDS BY THE CHOROID PLEXUS IN VITRO. Am J Physiol. 1965 Apr;208:666–673. doi: 10.1152/ajplegacy.1965.208.4.666. [DOI] [PubMed] [Google Scholar]
  12. Takemori A. E., Stenwick M. W. Studies on the uptake of morphine by the choroid plexus in vitro. J Pharmacol Exp Ther. 1966 Dec;154(3):586–594. [PubMed] [Google Scholar]
  13. Tochino Y., Schanker L. S. Transport of serotonin and norepinephrine by the rabbit choroid plexus in vitro. Biochem Pharmacol. 1965 Nov;14(11):1557–1566. doi: 10.1016/0006-2952(65)90010-9. [DOI] [PubMed] [Google Scholar]
  14. WELCH K. Active transport of iodide by choroid plexus of the rabbit in vitro. Am J Physiol. 1962 Apr;202:757–760. doi: 10.1152/ajplegacy.1962.202.4.757. [DOI] [PubMed] [Google Scholar]
  15. WELCH K. Concentration of thiocyanate by the choroid plexus of the rabbit in vitro. Proc Soc Exp Biol Med. 1962 Apr;109:953–954. doi: 10.3181/00379727-109-27389. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES