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. 1995 Jan;482(P):27S–30S. doi: 10.1113/jphysiol.1995.sp020561

Vesicular targeting and the control of ion secretion in epithelial cells: implications for cystic fibrosis.

S A Cunningham 1, R A Frizzell 1, A P Morris 1
PMCID: PMC1334231  PMID: 7730972

Abstract

Non-polarized HT-29 colonic epithelial cells fail to respond to cyclic AMP-generating agonists with increases in plasma membrane anion conduction. Radio-isotopic efflux and patch-clamp experiments revealed that both undifferentiated and differentiated HT-29 colonocytes possess volume- and Ca(2+)-activated Cl- channels. However, only within the apical plasma membranes of the latter were cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channels found. CFTR was expressed equally well in both non-polarized and polarized colonocytes. Lack of CFTR-dependent anion conduction was shown to be the result of CFTR retention within a peripheral intracellular compartment. We demonstrate that upon polarization, CFTR moves to the apical plasma membrane via a Brefeldin A (BFA)-sensitive intracellular trafficking pathway.

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Selected References

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