Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1975 Apr;15(4):299–306. doi: 10.1016/S0006-3495(75)85820-6

Pulsed nuclear magnetic resonance study of 17-O, 2-D, and 1-H of water in frog striated muscle.

M M Civan, M Shporer
PMCID: PMC1334691  PMID: 1079145

Abstract

Whole gastrocnemius muscles were incubated in Ringer's solution enriched with H2-17O; the paired contralateral gastrocnemius muscles were incubated in a similar solution enriched with deuterons, as well. Subsequently, the longitudinal relaxation times (T1) were measured 17-O, 2-D, and 1-H, both at 8.1 MHz and at 4.3 MHz. The results indicate that: (a) the absolute values of T1 characterizing the three nuclides are different in muscle and pure water. (b) the longitudinal relaxation rates of all three have an identical frequency dependence over the range studied, (c) the ratio (T1)2D/(T1)17ois the same in muscle water and pure water, while the ratio (T1)1H/(T1)17o is 2.1 times greater in pure water than it is in muscle water, and (d) 30-49 percent substitution of 2-D for 1-H has very little effect on the spin-lattice relaxation of tissue water protons. These data suggest that muscle water is in rapid exchange between a small fraction of immobilized molecules and a large fraction of free water. The results render unlikely the possibility that hypothetical ordering of muscle water significantly contributes to its longitudinal relaxation.

Full text

PDF
299

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRATTON C. B., HOPKINS A. L., WEINBERG J. W. NUCLEAR MAGNETIC RESONANCE STUDIES OF LIVING MUSCLE. Science. 1965 Feb 12;147(3659):738–739. doi: 10.1126/science.147.3659.738. [DOI] [PubMed] [Google Scholar]
  2. Belton P. S., Jackson R. R., Packer K. J. Pulsed NMR studies of water in striated muscle. I. Transverse nuclear spin relaxation times and freezing effects. Biochim Biophys Acta. 1972 Nov 24;286(1):16–25. doi: 10.1016/0304-4165(72)90084-0. [DOI] [PubMed] [Google Scholar]
  3. Civan M. M., Podolsky R. J. Contraction kinetics of striated muscle fibres following quick changes in load. J Physiol. 1966 Jun;184(3):511–534. doi: 10.1113/jphysiol.1966.sp007929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Civan M. M., Shporer M. 17 O nuclear magnetic resonance spectrum of H 2 17 O in frog striated muscle. Biophys J. 1972 Apr;12(4):404–413. doi: 10.1016/S0006-3495(72)86092-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Civan M. M., Shporer M. Pulsed NMR studies of 17O from H2 17O in frog striated muscle. Biochim Biophys Acta. 1974 Apr 22;343(2):399–408. doi: 10.1016/0304-4165(74)90104-4. [DOI] [PubMed] [Google Scholar]
  6. Fung B. M., McGaughy T. W. The state of water in muscle as studied by pulsed NMR. Biochim Biophys Acta. 1974 May 24;343(3):663–673. doi: 10.1016/0304-4165(74)90287-6. [DOI] [PubMed] [Google Scholar]
  7. Hazlewood C. F., Chang D. C., Nichols B. L., Woessner D. E. Nuclear magnetic resonance transverse relaxation times of water protons in skeletal muscle. Biophys J. 1974 Aug;14(8):583–606. doi: 10.1016/S0006-3495(74)85937-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koenig S. H., Schillinger W. E. Nuclear magnetic relaxation dispersion in protein solutions. I. Apotransferrin. J Biol Chem. 1969 Jun 25;244(12):3283–3289. [PubMed] [Google Scholar]
  9. Koenig S. H., Schillinger W. E. Nuclear magnetic relaxation dispersion in protein solutions. II. Transferrin. J Biol Chem. 1969 Dec 10;244(23):6520–6526. [PubMed] [Google Scholar]
  10. Woessner D. E., Snowden B. S., Jr A pulsed NMR study of dynamics and ordering of water molecules in interfacial systems. Ann N Y Acad Sci. 1973 Mar 30;204:113–124. doi: 10.1111/j.1749-6632.1973.tb30774.x. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES