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. 1975 May;15(5):417–434. doi: 10.1016/S0006-3495(75)85827-9

Transmembrane Lipid Migration in Planar Asymmetric Bilayer Membranes

Dennis Sherwood, M Montal
PMCID: PMC1334714  PMID: 19211014

Abstract

Planar asymmetric bilayer membranes, formed by apposing a monolayer of the neutral lipid glyceroldioleate (GDO) with one of the negatively charged lipid oleyl acid phosphate (OAP), were used to measure the rate of transmembrane OAP migration. The assay for this lipid flip-flop was the interaction of Ca2+ ions with negatively charged lipids which causes membranes to break: when Ca2+ is added to the compartment limited initially by the neutral lipid, flip-flop of the charged lipid eventually results in membrane breakdown. At 22 ± 2°C, in the absence of an externally applied electric field, an upper limit to the half time of OAP flip-flop was measured as 18.7 h, with a tentative lower limit of 14.4 h.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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