Abstract
The yield of fluorescence in Chlorella from a 7 ns pulse of light is found to decrease gradually as a function of the number of hits in the photosynthetic units. The fivefold decrease in yield is spread over some three orders of magnitude of pulse energy and strongly suggests another random process in addition to that of photon absorption. Evidence supports the view that this random process is not in the time but in the spatial domain. The model used to fit the data is that of a unit with multiple traps for the singlet excitation. An excitation is captured by an open trap or destroyed by a filled trap with equal probability. These studies give evidence for the connectivity of the photosynthetic energy transfer apparatus on the short time scale. The short fluorescence lifetimes following picosecond pulse excitation of photosynthetic systems reported by several laboratories may be explained by the effect of multiple excitations.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Borisov A. Y., Il'ina M. D. The fluorescence lifetime and energy migration mechanism in photosystem I of plants. Biochim Biophys Acta. 1973 May 30;305(2):364–371. doi: 10.1016/0005-2728(73)90182-5. [DOI] [PubMed] [Google Scholar]
- Campillo A. J., Shapiro S. L., Kollman V. H., Winn K. R., Hyer R. C. Picosecond exciton annihilation in photosynthetic systems. Biophys J. 1976 Jan;16(1):93–97. doi: 10.1016/S0006-3495(76)85666-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diner B., Mauzerall D. Feedback controlling oxygen production in a cross-reaction between two photosystems in photosynthesis. Biochim Biophys Acta. 1973 May 30;305(2):329–352. doi: 10.1016/0005-2728(73)90180-1. [DOI] [PubMed] [Google Scholar]
- Herron H. A., Mauzerall D. The development of photosynthesis in a greening mutant of chlorella and an analysis of the light saturation curve. Plant Physiol. 1972 Jul;50(1):141–148. doi: 10.1104/pp.50.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joliot P., Joliot A., Kok B. Analysis of the interactions between the two photosystems in isolated chloroplasts. Biochim Biophys Acta. 1968 Apr 2;153(3):635–652. doi: 10.1016/0005-2728(68)90191-6. [DOI] [PubMed] [Google Scholar]
- Kollman V. H., Shapiro S. L., Campillo A. J. Photosynthetic studies with a 10-psec resolution streak camera. Biochem Biophys Res Commun. 1975 Apr 21;63(4):917–923. doi: 10.1016/0006-291x(75)90656-7. [DOI] [PubMed] [Google Scholar]
- Mauzerall D. Light-induced fluorescence changes in Chlorella, and the primary photoreactions for the production of oxygen. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1358–1362. doi: 10.1073/pnas.69.6.1358. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seibert M., Alfano R. R. Probing photosynthesis on a picosecond time scale. Evidence for photosystem I and photosystem II fluorescence in chloroplasts. Biophys J. 1974 Apr;14(4):269–283. doi: 10.1016/S0006-3495(74)85915-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang R. T., Myers J. Energy transfer between photosynthetic units analyzed by flash oxygen yield vs. flash intensity. Photochem Photobiol. 1973 May;17(5):321–332. doi: 10.1111/j.1751-1097.1973.tb06360.x. [DOI] [PubMed] [Google Scholar]
- Yu W., Ho P. P., Alfano R. R., Seibert M. Fluorescent kinetics of chlorophyll in photosystems I and II enriched fractions of spinach. Biochim Biophys Acta. 1975 Apr 14;387(1):159–164. doi: 10.1016/0005-2728(75)90060-2. [DOI] [PubMed] [Google Scholar]