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. 1976 Apr;16(4):357–365. doi: 10.1016/S0006-3495(76)85693-7

Ion etching of tobacco mosaic virus and T4 bacteriophage.

I Bendet, N Rizk
PMCID: PMC1334847  PMID: 766845

Abstract

A technique for the etching of biological materials by accelerated nitrogen ions is described. Upon exposure to such a beam, tobacco mosaic virus becomes thinner in diameter and the head of T4 becomes smaller. Experiments on radioactively labeled T4 suggest that the particle's protein is more susceptible to removal by the incident ions, and that the residual material is predominantly nucleic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beer M., Frank J., Hanszen K. J., Kellenberger E., Williams R. C. The possibilities and prospects of obtaining high-resolution information (below 30 A) on biological material using the electron microscope. Some comments and reports inspired by an EMBO workshop held at Gais, Switzerland, October 1973. Q Rev Biophys. 1974 May;7(2):211–238. doi: 10.1017/s0033583500001414. [DOI] [PubMed] [Google Scholar]
  2. Dubochet J. Carbon loss during irradiation of T4 bacteriophages and E. coli bacteria in electron microscopes. J Ultrastruct Res. 1975 Aug;52(2):276–288. doi: 10.1016/s0022-5320(75)80118-3. [DOI] [PubMed] [Google Scholar]
  3. Georgopoulos C. P., Hendrix R. W., Casjens S. R., Kaiser A. D. Host participation in bacteriophage lambda head assembly. J Mol Biol. 1973 May 5;76(1):45–60. doi: 10.1016/0022-2836(73)90080-6. [DOI] [PubMed] [Google Scholar]
  4. Glaeser R. M. Limitations to significant information in biological electron microscopy as a result of radiation damage. J Ultrastruct Res. 1971 Aug;36(3):466–482. doi: 10.1016/s0022-5320(71)80118-1. [DOI] [PubMed] [Google Scholar]
  5. Glaeser R. M., Thomas G. Application of electron diffraction to biological electron microscopy. Biophys J. 1969 Sep;9(9):1073–1099. doi: 10.1016/S0006-3495(69)86437-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KOBAYASHI K., SAKAOKU K. IRRADIATION CHANGES IN ORGANIC POLYMERS AT VARIOUS ACCELERATING VOLTAGES. Lab Invest. 1965 Jun;14:1097–1114. [PubMed] [Google Scholar]
  7. REIMER L. IRRADIATION CHANGES IN ORGANIC AND INORGANIC OBJECTS. Lab Invest. 1965 Jun;14:1082–1096. [PubMed] [Google Scholar]
  8. STEERE R. L. Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol. 1957 Jan 25;3(1):45–60. doi: 10.1083/jcb.3.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Thach R. E., Thach S. S. Damage to biological samples caused by the electron beam during electron microscopy. Biophys J. 1971 Feb;11(2):204–210. doi: 10.1016/S0006-3495(71)86208-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Williams R. C., Fisher H. W. Electron microscopy of tobacco mosaic virus under conditions of minimal beam exposure. J Mol Biol. 1970 Aug 28;52(1):121–123. doi: 10.1016/0022-2836(70)90181-6. [DOI] [PubMed] [Google Scholar]

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