Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1976 May;16(5):503–516. doi: 10.1016/S0006-3495(76)85705-0

Phase determination of x-ray reflections for membrane-type systems with constant fluid density.

J B Stamatoff, S Krimm
PMCID: PMC1334871  PMID: 1276379

Abstract

A new technique for phase determination of X-ray reflections from symmetric structures is presented. This method, involving comparison of intensity data from structures with variable fluid layer thickness and constant fluid electron density, permits computation of phase angles, scaling factors, and origin reflection values independently. Possible sources of error inherent in other methods of phase determination are thereby eliminated. Results of the application of this method to model structures and to myelin data are reported. Advantages of the technique, which tests all possible phase angle combinations in a rapid fashion, are discussed.

Full text

PDF
503

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaurock A. E. Structure of the nerve myelin membrane: proof of the low-resolution profile. J Mol Biol. 1971 Feb 28;56(1):35–52. doi: 10.1016/0022-2836(71)90082-9. [DOI] [PubMed] [Google Scholar]
  2. Corless J. M. Lamellar structure of bleached and unbleached rod photoreceptor membranes. Nature. 1972 May 26;237(5352):229–231. doi: 10.1038/237229a0. [DOI] [PubMed] [Google Scholar]
  3. Luzzati V., Tardieu A., Taupin D. A pattern-recognition approach to the phase problem: application to the X-ray diffraction study of biological membranes and model systems. J Mol Biol. 1972 Feb 28;64(1):269–286. doi: 10.1016/0022-2836(72)90335-x. [DOI] [PubMed] [Google Scholar]
  4. McIntosh T. J., Worthington C. R. Direct determination of the lamellar structure of peripheral nerve myelin at low resolution (17 A). Biophys J. 1974 May;14(5):363–386. doi: 10.1016/S0006-3495(74)85922-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Moody M. F. Letter: Structure determination of membranes in swollen lamellar systems. Biophys J. 1974 Sep;14(9):697–702. doi: 10.1016/S0006-3495(74)85945-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pape E. H. X-ray small angle scattering. A new deconvolution method for evaluating electron density distributions from small angle scattering diagrams. Biophys J. 1974 Apr;14(4):284–294. doi: 10.1016/S0006-3495(74)85916-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rand R. P., Luzzati V. X-ray diffraction study in water of lipids extracted from human erythrocytes: the position of cholesterol in the lipid lamellae. Biophys J. 1968 Jan;8(1):125–137. doi: 10.1016/S0006-3495(68)86479-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stamatoff J. B., Krimm S., Harvie N. R. X-ray diffraction studies of human erythrocyte membrane structure. Proc Natl Acad Sci U S A. 1975 Feb;72(2):531–534. doi: 10.1073/pnas.72.2.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Worthington C. R., King G. I. Electron density profiles of nerve myelin. Nature. 1971 Nov 19;234(5325):143–145. doi: 10.1038/234143a0. [DOI] [PubMed] [Google Scholar]
  10. Worthington C. R., King G. I., McIntosh T. J. Direct structure determination of multilayered membrane-type systems which contain fluid layers. Biophys J. 1973 May;13(5):480–494. doi: 10.1016/S0006-3495(73)86001-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Worthington C. R., McIntosh T. J. Direct determination of the lamellar structure of peripheral nerve myelin at moderate resolution (7A). Biophys J. 1974 Oct;14(10):703–729. doi: 10.1016/S0006-3495(74)85946-1. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES