Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1976 Aug;16(8):883–889. doi: 10.1016/S0006-3495(76)85738-4

Rate of DNA chain elongation in ultraviolet light-irradiated mammalian cells as estimated by a bromodeoxyuridine photolysis method.

L F Povirk, R B Painter
PMCID: PMC1334913  PMID: 938728

Abstract

Using a new method, we show that, when the DNA chain elongation rate is measured for short time periods, ultraviolet light does not decrease this rate. The method is based on the photolysis of bromouracil-containing DNA by 313 nm light and alkaline sucrose gradients.

Full text

PDF
883

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buhl S. N., Setlow R. B., Regan J. D. Steps in DNA chain elongation and joining after ultra-violet irradiation of human cells. Int J Radiat Biol Relat Stud Phys Chem Med. 1972 Nov;22(5):417–424. doi: 10.1080/09553007214551301. [DOI] [PubMed] [Google Scholar]
  2. Buhl S. N., Stillman R. M., Setlow R. B., Regan J. D. DNA chain elongation and joining in normal human and xeroderma pigmentosum cells after ultraviolet irradiation. Biophys J. 1972 Sep;12(9):1183–1191. doi: 10.1016/S0006-3495(72)86154-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cleaver J. E., Thomas G. H. Single strand interruptions in DNA and the effects of caffeine in Chinese hamster cells irradiated with ultraviolet light. Biochem Biophys Res Commun. 1969 Jul 23;36(2):203–208. doi: 10.1016/0006-291x(69)90315-5. [DOI] [PubMed] [Google Scholar]
  4. Edenberg H. J., Huberman J. A. Eukaryotic chromosome replication. Annu Rev Genet. 1975;9:245–284. doi: 10.1146/annurev.ge.09.120175.001333. [DOI] [PubMed] [Google Scholar]
  5. Fujiwara Y., Kondo T. Caffeine-sensitive repair of ultraviolet light-damaged DNA of mouse L cells. Biochem Biophys Res Commun. 1972 May 12;47(3):557–564. doi: 10.1016/0006-291x(72)90915-1. [DOI] [PubMed] [Google Scholar]
  6. Gautschi J. R., Kern R. M. DNA replication in mammalian cells in the presence of cycloheximide. Exp Cell Res. 1973 Jul;80(1):15–26. doi: 10.1016/0014-4827(73)90270-x. [DOI] [PubMed] [Google Scholar]
  7. Lehmann A. R., Ormerod M. G. The replication of DNA in murine lymphoma cells (L5178Y). I. Rate of replication. Biochim Biophys Acta. 1970 Mar 19;204(1):128–143. doi: 10.1016/0005-2787(70)90496-x. [DOI] [PubMed] [Google Scholar]
  8. Lehmann A. R. Postreplication repair of DNA in ultraviolet-irradiated mammalian cells. J Mol Biol. 1972 May 28;66(3):319–337. doi: 10.1016/0022-2836(72)90418-4. [DOI] [PubMed] [Google Scholar]
  9. Painter R. B., Schaefer A. W. Rate of synthesis along replicons of different kinds of mammalian cells. J Mol Biol. 1969 Nov 14;45(3):467–479. doi: 10.1016/0022-2836(69)90306-4. [DOI] [PubMed] [Google Scholar]
  10. Povirk L. F., Painter R. B. The effect of 313 nanometer light on initiation of replicons in mammalian cell DNA containing bromodeoxyuridine. Biochim Biophys Acta. 1976 May 19;432(3):267–272. doi: 10.1016/0005-2787(76)90135-0. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES