Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1976 Sep;16(9):1109–1113. doi: 10.1016/S0006-3495(76)85759-1

Branched bimolecular lipid membranes.

H Schindler, G Feher
PMCID: PMC1334949  PMID: 963207

Abstract

Branched bimolecular lipid membranes separating three and four aqueous phases have been formed. The procedure is based on the technique of Montal and Mueller generalized to three and four lipid surface films spanning an appropriate aperture. The technique to produce Teflon structures for the mechanical support of branched bilayers is presented. The existence of the branched bilayer was established by measuring the specific capacity, specific resistance, and the gramicidin-induced single channel conductance of each branch. These structures should facilitate the study of transport properties of ionophores and other molecules and may also serve as model systems for the study of cell fusion.

Full text

PDF
1109

Images in this article

1112FIGURE 3
on p.1112

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bamberg E., Läuger P. Temperature-dependent properties of gramicidin A channels. Biochim Biophys Acta. 1974 Oct 29;367(2):127–133. doi: 10.1016/0005-2736(74)90037-6. [DOI] [PubMed] [Google Scholar]
  2. Benz R., Fröhlich O., Läuger P., Montal M. Electrical capacity of black lipid films and of lipid bilayers made from monolayers. Biochim Biophys Acta. 1975 Jul 3;394(3):323–334. doi: 10.1016/0005-2736(75)90287-4. [DOI] [PubMed] [Google Scholar]
  3. FARQUHAR M. G., PALADE G. E. Junctional complexes in various epithelia. J Cell Biol. 1963 May;17:375–412. doi: 10.1083/jcb.17.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Haydon D. A., Hladky S. B. Ion transport across thin lipid membranes: a critical discussion of mechanisms in selected systems. Q Rev Biophys. 1972 May;5(2):187–282. doi: 10.1017/s0033583500000883. [DOI] [PubMed] [Google Scholar]
  5. Hladky S. B., Haydon D. A. Ion transfer across lipid membranes in the presence of gramicidin A. I. Studies of the unit conductance channel. Biochim Biophys Acta. 1972 Aug 9;274(2):294–312. doi: 10.1016/0005-2736(72)90178-2. [DOI] [PubMed] [Google Scholar]
  6. Montal M. Formation of bimolecular membranes from lipid monolayers. Methods Enzymol. 1974;32:545–554. doi: 10.1016/0076-6879(74)32053-8. [DOI] [PubMed] [Google Scholar]
  7. Montal M., Mueller P. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3561–3566. doi: 10.1073/pnas.69.12.3561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mueller P., Rudin D. O. Development of K+-Na+ discrimination in experimental bimolecular lipid membranes by macrocyclic antibiotics. Biochem Biophys Res Commun. 1967 Feb 21;26(4):398–404. doi: 10.1016/0006-291x(67)90559-1. [DOI] [PubMed] [Google Scholar]
  9. Satir B., Schooley C., Satir P. Membrane fusion in a model system. Mucocyst secretion in Tetrahymena. J Cell Biol. 1973 Jan;56(1):153–176. doi: 10.1083/jcb.56.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. VAN DEN BERG H. J. A NEW TECHNIQUE FOR OBTAINING THIN LIPID FILMS SEPARATING TWO AQUEOUS MEDIA. J Mol Biol. 1965 May;12:290–291. doi: 10.1016/s0022-2836(65)80302-3. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES