ABSTRACT
The HLA‐A*24:677 allele differs from HLA‐A*24:02:01:01 by a single non‐synonymous nucleotide change in exon 2.
Keywords: HLA genotyping, HLA‐A*24:677, IPD‐IMGT/HLA database, novel allele, sequence‐based typing
The HLA genes are the most polymorphic group known in the human genome. In this report, we describe a novel HLA‐A allele, designated HLA‐A*24:677, which was identified by Sanger dideoxy nucleotide sequencing of a sample from a Chinese haematopoietic stem cell donor.
Using the DNA Extraction Reagent Kit (Qiagen, Hilden, Germany), genomic DNA was extracted from whole blood. After extraction, the DNA was subjected to HLA genotyping for HLA‐A, ‐B, ‐C, ‐DRB1, ‐DQB1 and ‐DPB1 loci with the specific sequencing primers (CATB, HLA‐SBT Kits, Shanghai, China). Informed consent was obtained from the subject involved.
The HLA‐A*24:677 allele had one nucleotide change when compared to the HLA‐A*24:02:01:01 allele in exon 2 at position 223 where T → G (TGG → GGG) in codon 51, resulting in an amino acid change from Tryptophan to Glycine (Figure 1).
FIGURE 1.
The exon 2 nucleotide sequence of the HLA‐A*24:677 allele compared with the closely related sequence of HLA‐A*24:02:01:01. Dashes (‐) indicate identity with the HLA‐A*24:02:01:01 allele. The HLA‐A*24:677 allele had one nucleotide change when compared to the closely related HLA‐A*24:02:01:01 allele in exon 2 at position 223 where T → G (TGG → GGG) in codon 51, resulting in an amino acid change from Tryptophan to Glycine.
The name HLA‐A*24:677 has been officially assigned by the WHO Nomenclature Committee for Factors of the HLA System in December 2025 (submission number HWS10102016) [1]. This follows the agreed policy that subjects to conditions stated in the most recent Nomenclature Report, names will be assigned to new sequences as they are identified. Lists of such names will be published in the following WHO Nomenclature Report [2].
Author Contributions
En‐xiang Zhou designed the study and analysed the data; Kun Shao and Xiao‐hong Cai wrote the manuscript; Pei‐pei Ding and Li Zhou provided genetic laboratory data. All authors read and approved the final version of the manuscript.
Funding
The authors have nothing to report.
Conflicts of Interest
The authors declare no conflicts of interest.
Acknowledgements
We appreciate Shanghai Tissue bank Biotechnology Co. Ltd. for assistance with experiment and data analysis.
Data Availability Statement
The sequence is freely available in the IPD‐IMGT/HLA Database.
References
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Data Availability Statement
The sequence is freely available in the IPD‐IMGT/HLA Database.