Methods
Tissues from single, wild-collected individuals were used for this study. DNA extraction was performed using the Qiagen DNEasy genomic extraction kit using the standard process. Paired-end sequencing libraries were constructed using the Illumina TruSeq kit according to the manufacturer’s instructions. The libraries were sequenced on an Illumina Hi-Seq platform in paired-end, 2 × 150bp format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger et al. 2014). The trimmed sequence was assembled by SPAdes v3.15.4 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras et al. 2021).
Results and Data Availability
| taxname | accession |
|---|---|
| Micromeria benthamii | JBWICF000000000 |
| Micromeria densiflora | JBWICE000000000 |
| Micromeria ericifolia | JBWICM000000000 |
| Micromeria glomerata | JBWICL000000000 |
| Micromeria gomerensis | JBWICG000000000 |
| Micromeria helianthemifolia | JBWICK000000000 |
| Micromeria herpyllomorpha | JBWICH000000000 |
| Micromeria hierrensis | JBWICI000000000 |
| Micromeria hochreutineri | JBXGFH000000000 |
| Micromeria lachnophylla | JBWIBY000000000 |
| Micromeria lanata | JBWIBZ000000000 |
| Micromeria lasiophylla | JBXGFL000000000 |
| Micromeria lepida | JBWICB000000000 |
| Micromeria leucantha | JBWICD000000000 |
| Micromeria madeirensis | JBWICJ000000000 |
| Micromeria pedro-luisii | JBXGEP000000000 |
| Micromeria pineolens | JBXGFJ000000000 |
| Micromeria rivas-martinezii | JBXGFG000000000 |
| Micromeria tenensis | JBXGFI000000000 |
| Micromeria teneriffae | JBXGEO000000000 |
| Micromeria tenuis | JBXGEN000000000 |
| Micromeria tragothymus | JBXGEM000000000 |
Funding
Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa.
Footnotes
We present complete genome sequences of 22 species of Micromeria.
REFERENCES
- Bankevich Anton, Nurk Sergey, Antipov Dmitry, et al. 2012. “SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing.” Journal of Computational Biology. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bolger Anthony M., Lohse Marc, and Usadel Bjoern. 2014. “Trimmomatic: A Flexible Trimmer for Illumina Sequence Data.” Bioinformatics 30 (15): 2114–20. 10.1093/bioinformatics/btu170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kieras M, O’Neill K, and Pirro S. 2021. “Zanfona, a Genome Assembly Finishing Tool for Paired-End Illumina Reads.” https://github.com/zanfona734/zanfona.