Correction: Robo1/2 regulate follicle atresia through manipulating granulosa cell apoptosis in mice

Correction to: Scientific Reports 10.1038/srep09720, published online 19 May 2015

This Article contains errors in Figures 3 and 7.

In Figure 3B, the upper panel of wild-type ovarian sections contains an inadvertent duplication. The correct Figure 3 and accompanying legend appear below.

Fig. 3.

The Robo1/2^+/− knockout ovary promoted follicle maturation. (A): The two groups of ovaries from the 10-week-old wild-typeand Robo1/2^+/− knockoutmice. (B): H&E staining of ovarian vertical sections from 4-week-old wild-type and Robo1/2^+/− knockout mice. (C): H&E staining of ovarian vertical sections from 10-week-old wild-type and Robo1/2^+/− knockout mice. (B'–C'): The diagrams show that the number of oocyte induced by hyperstimulation are not apparently changed in the 4-week-old (B', WT n = 8, Robo1/2 n = 9) but increase in 10-week-old (C', WT n = 6, Robo1/2 n = 3) Robo1/2^+/− knockout mice, respectively. (D–E): Bar chart showing the changes in 4-week (D, WT n = 17, Robo1/2 n = 15) and 10-week (E, WT n = 8, Robo1/2 n = 10) ovarian follicle number in terms of follicle stage, including primary follicles, secondary follicles, and corpus lutea. The total follicle number was also counted. ***p < 0.001 indicates a significant difference between the wild-type and Robo1/2^+/− knockout groups. Abbreviations: WT, wild-type; ROBO1/2^+/− mice, double Robo1/2^+/− knockout. Scale bars = 200 μm in (A) and 500 μm in (B and C).

In Figure 7, panel designations G-L do not correspond to the descriptions in the accompanying legend. Additionally, the number of secondary follicles from Robo1/2^+/− mice analysed to generate the bar chart on CD34 expression is incorrectly stated. Furthermore, Figures 7D, E and F show primary, secondary and mature follicles, respectively, from the same ovary; this is not clearly stated in the Article. For improved clarity, green dotted lines have been added to Figures 7A-F to delineate follicle boundaries.

The correct Figure 7 and accompanying legend appear below.

Fig. 7.

The increase in angiogenesis in ovaries of the Robo1/2^+/− knockout mice. (A–C): The immunocytochemistry against CD34 was performed on the vertical sections of the wild-type mouse ovary. The photographs were taken at the site of primary (A), secondary (B), and mature (C) follicles. (D–F): The immunochemistry against CD34 was performed on the vertical sections of the Robo1/2^+/− knockout mouse ovary. The photographs were taken at the site of primary (D), secondary (E), and mature (F) follicles. (G): The bar chart showing the comparison of integral optical density (IOD) for CD34 expression in primary (WT n = 10, Robo1/2 n = 7), secondary (WT n = 11, Robo1/2 n = 1), and mature follicles (WT n = 5, Robo1/2 n = 5). (H–I): The fluorescent immunostaining against SMA was performed on the vertical sections of the wild-type (H) and Robo1/2^+/− knockout (I) mouse ovaries, followed by a DAPI counterstain. (H1–I1): The high-magnification images from the sites indicated by dotted squares in (G) and (H), respectively. (J): The bar chart showing the comparison of IOD for SMA expression in the primary (WT n = 5, Robo1/2 n = 5), secondary (WT n = 3, Robo1/2 n = 5), and mature (WT n = 4, Robo1/2 n = 4) follicles. (K–L): The fluorescent immunostaining against CD31 was performed on the vertical sections of the wild-type (K) and Robo1/2^+/− knockout (L) mouse ovaries followed by a DAPI counterstain. Abbreviations: IOD, integral optical density; WT, wild-type; ROBO1/2^+/− mice, double Robo1/2^+/− knockout. Scale bars = 20 μm in (A–F), 200 μm in (H–I), 50 μm in (H1–I1), and 50 μm in (K–L).

These changes do not affect the conclusions of the Article.