Abstract
Blood stored at 4 C in ACD or CPD solution develops microaggregates composed primarily of fibrin and platelets. This debris has been implicated in the pathogenesis of posttraumatic pulmonary insufficiency in man. Recent work indicates that gentle agitation of the blood during storage appears to decrease debris formation. These studies were undertaken to establish more clearly the effect of agitation on debris formation. Blood was drawn in CPD from healthy young males, non-aspirin ingesting donors and stored at 4 C. One-half of the bags were gently and continuously agitated for 21 days and the other half remained stationary. At the end of the storage period, platelet counts and screen filtration pressures were measured. Agitated blood showed significantly less debris formation and significantly higher platelet counts. Gentle agitation was shown to be an effective method for preventing debris formation in banked blood.
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Selected References
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- Arrington P., McNamara J. J. Mechanism of microaggregate formation in stored blood. Ann Surg. 1974 Feb;179(2):146–148. doi: 10.1097/00000658-197402000-00005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN P., GARDNER F. H., BARNETT G. O. Reclassification of the thrombocytopenias by the Cr51-labeling method for measuring platelet life span. N Engl J Med. 1961 Jun 29;264:1350–concl. doi: 10.1056/NEJM196106292642607. [DOI] [PubMed] [Google Scholar]
- Hissen W., Swank R. L. Screen filtration pressure and pulmonary hypertension. Am J Physiol. 1965 Oct;209(4):715–722. doi: 10.1152/ajplegacy.1965.209.4.715. [DOI] [PubMed] [Google Scholar]
- JENEVEIN E. P., Jr, WEISS D. L. PLATELET MICROEMBOLI ASSOCIATED WITH MASSIVE BLOOD TRANSFUSION. Am J Pathol. 1964 Aug;45:313–325. [PMC free article] [PubMed] [Google Scholar]
- McNamara J. J., Burran E. L., Larson E., Omiya G., Suehiro G., Yamase H. Effect of debris in stored blood on pulmonary microvasculature. Ann Thorac Surg. 1972 Aug;14(2):133–139. doi: 10.1016/s0003-4975(10)65211-3. [DOI] [PubMed] [Google Scholar]
- McNamara J. J., Burran E. L., Suehiro G. Effective filtration of banked blood. Surgery. 1972 Apr;71(4):594–597. [PubMed] [Google Scholar]
- McNamara J. J., Molot M. D., Stremple J. F. Screen filtration pressure in combat casualties. Ann Surg. 1970 Sep;172(3):334–341. doi: 10.1097/00000658-197009000-00003. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SWANK R. L. Alteration of blood on storage: measurement of adhesiveness of "aging" platelets and leukocytes and their removal by filtration. N Engl J Med. 1961 Oct 12;265:728–733. doi: 10.1056/NEJM196110122651503. [DOI] [PubMed] [Google Scholar]